Chapter 14 Preparation of Protoplasts of Plant Cells

Landgren, Craig R.

doi:10.1016/s0091-679x(08)62018-x

Cited by 4 publications

(1 citation statement)

References 28 publications

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“…The purification of protoplasts and perfect removal of the residues of cell walls, damaged protoplasts and isolation enzymes are the condition of further cultivation of protoplasts and are done by repeated centrifugation (LANDGREN 1978).…”

Section: Enzymesmentioning

confidence: 99%

Protoplast cultures and protoplast fusion focused on Brassicaceae: A review

Navrátilová¹

2004

Hort. Sci. (Prague)

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ABSTRACT:The subjects of this article are protoplast isolations and protoplast fusions, in particular their history, a review of factors influencing the protoplasts isolation and fusion, selection of hybrid plants and utilization of somatic hybrids in plant breeding. Somatic hybridization through protoplast fusion can overcome sexual incompatibility among plant species or genera; transfer genes of resistance to diseases (viral, bacterial, fungal), pests, herbicides and others stress factors; obtain cybrid plants; transfer cytoplasmic male sterility or incease content of secondary metabolites in hybrid plants. The article is focussed mainly on the family Brassicaceae because among representatives are significant crops for the human population. Various successful combination of intraspecific, interspecific and intergeneric protoplast fusion were reported between representatives of the family Brassicaceae with the genus Brassica which belonged to the first agricultural crops used for the isolation of protoplast.

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Section: Enzymesmentioning

confidence: 99%