Chapter 4 Role of Antioxidants and Antifreeze Proteins in Cryopreservation/Vitrification

Kim, Seul Ki; Youm, Hye Won; Lee, Jung Ryeol; Suh, Chang Suk

doi:10.1007/978-1-4939-6828-2_4

Cited by 4 publications

(1 citation statement)

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“…Mainly as antioxidants, their use has been focused on the preservation of reproductive cells or embryos [39]. These molecules are ice-binding proteins that prevent water from freezing by adsorbing to the ice surface and stopping the growth of diminutive ice crystals to large crystals in a non-colligative manner.…”

Section: 1choice Of Cpa Solutionmentioning

confidence: 99%

Advances in the slow freezing cryopreservation of microencapsulated cells

Gurruchaga

Burgo

Hernández

et al. 2018

Journal of Controlled Release

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Over the past few decades, the use of cell microencapsulation technology has been promoted for a wide range of applications as sustained drug delivery systems or as cells containing biosystems for regenerative medicine. However, difficulty in their preservation and storage has limited their availability to healthcare centers. Because the preservation in cryogenic temperatures poses many biological and biophysical challenges and that the technology has not been well understood, the slow cooling cryopreservation, which is the most used technique worldwide, has not given full measure of its full potential application yet. This review will discuss the different steps that should be understood and taken into account to preserve microencapsulated cells by slow freezing in a successful and simple manner. Moreover, it will review the slow freezing preservation of alginate-based microencapsulated cells and discuss some recommendations that the research community may pursue to optimize the preservation of microencapsulated cells, enabling the therapy translate from bench to the clinic.

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Section: 1choice Of Cpa Solutionmentioning

confidence: 99%