Chapter 6 Sedatives and hypnotics

Kræmer, Thomas; Maurer, Hans H.

doi:10.1016/s1567-7192(00)80056-x

Cited by 5 publications

(5 citation statements)

References 167 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BTA and TBA both exist in keto and enol forms. The α‐carbon in BTA/TBA is very acidic with pK a value of 4.01 15 . This distinctive structure imparts specific chemical properties to BTA/TBA, including its ability to react with aldehydes and ketones.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Urease inhibition and molecular docking studies on transition metal complexes of ligands derived from barbituric and thiobarbituric acids

Shah,

Khan,

Halim

et al. 2024

Applied Organom Chemis

View full text Add to dashboard Cite

In this paper, we have examined eight new first‐row transition metal complexes (1–8). Mutli‐donor ligands based on barbituric and thiobarbituric acids were used in complexation reactions. The synthesized complexes were analyzed using FT‐IR and UV–vis spectroscopy, elemental (CHN) analysis, and single‐crystal X‐ray diffraction analysis. These complexes exhibited significant urease enzyme inhibition with IC50 values in the range of 13.73 ± 1.08–43.21 ± 1.50 μM. We observed excellent binding orientation of these complexes in the active site of Urease. The molecular docking results correlate well with in vitro observations.

show abstract

Section: Resultsmentioning

confidence: 99%

“…The α-carbon in BTA/TBA is very acidic with pK a value of 4.01. 15 This distinctive structure imparts specific chemical…”

Section: Ligand and Complex Synthesesmentioning

confidence: 99%

Urease inhibition and molecular docking studies on transition metal complexes of ligands derived from barbituric and thiobarbituric acids

Shah,

Khan,

Halim

et al. 2024

Applied Organom Chemis

View full text Add to dashboard Cite

show abstract

“…5,16 However, these methods remain far from ideal, with complex sample preparation protocols, insufficient sensitivity and reports of temperature-induced degradation of certain benzodiazepines with GC/MS methods. 17 LC/MS/ MS can be used to analyse almost all drugs of abuse in biological fluids, with equal or better specificity, usually better sensitivity and simpler sample preparation than GC/MS. 18 Recently, laboratories have invested increasingly in LC/ MS/MS and it is set to become a core analytical tool.…”

Section: Discussionmentioning

confidence: 99%

Measurement of benzodiazepines in urine by liquid chromatography-tandem mass spectrometry: confirmation of samples screened by immunoassay

Glover

Allen

2009

Ann Clin Biochem

View full text Add to dashboard Cite

Background: Liquid chromatography linked to tandem mass spectrometry (LC/MS/MS) provides the ability to identify a range of benzodiazepines in accordance with European Union criteria and is an attractive method for the confirmation of benzodiazepines following immunoassay screening. Methods: An LC/MS/MS method to detect and quantitate the six most common benzodiazepines/metabolites (diazepam, nitrazepam, nordiazepam, oxazepam, temazepam and 7-aminonitrazepam) was developed together with a qualitative screening method for a further 11 benzodiazepines/metabolites. These methods were used for confirmation of 250 urine samples submitted for routine drug screening by immunoassay for benzodiazepines (100 samples positive for a benzodiazepine, assay cut-off .200 msg/L). Results: The lower limits of detection and quantitation were less than 2.5 and 5 mg/L for the six most common benzodiazepines. Recoveries ranged between 97% and 102% and calibration curves were linear to at least 4000 mg/L (r ¼ 0.99). Intra and inter-assay imprecision were ,10% (n ¼ 10) and ,20% (n ¼ 15), respectively. Confirmation of benzodiazepines using LC/MS/MS was achieved for 89% of the immunoassay-positive urine samples. Of the immunoassaynegative urine samples, 31% of these demonstrated a benzodiazepine using LC/MS/MS. Conclusion: The validated LC/MS/MS method developed is effective for the confirmation of immunoassay screening results for benzodiazepines. The lower limit of detection and assay specificity offers a longer window of detection and more detailed clinical information compared with immunoassay screening.

show abstract

“…The use of HPLC to separate the compounds before detection by MS avoids the temperature-induced degradation of certain BZPs that can occur when GC methods are used (10 ). Another major advantage of HPLC for analyzing BZPs is that no derivatization of the metabolites is required (11 ).…”

Section: Discussionmentioning

confidence: 99%

Liquid Chromatography–Tandem Mass Spectrometry for Detection of Low Concentrations of 21 Benzodiazepines, Metabolites, and Analogs in Urine: Method with Forensic Applications

et al. 2006

View full text Add to dashboard Cite

show abstract

Chapter 6 Sedatives and hypnotics

Cited by 5 publications

References 167 publications

Urease inhibition and molecular docking studies on transition metal complexes of ligands derived from barbituric and thiobarbituric acids

Urease inhibition and molecular docking studies on transition metal complexes of ligands derived from barbituric and thiobarbituric acids

Measurement of benzodiazepines in urine by liquid chromatography-tandem mass spectrometry: confirmation of samples screened by immunoassay

Liquid Chromatography–Tandem Mass Spectrometry for Detection of Low Concentrations of 21 Benzodiazepines, Metabolites, and Analogs in Urine: Method with Forensic Applications

Contact Info

Product

Resources

About