Chapter IX Identification of Mycoplasmas

Isolation ofMycoplasma synoviaefrom the red‐legged partridge (Alectoris rufa)

“…The biochemical characteristics of the isolations were tested using the methods described by Freundt et al (1979). Mycoplasma isolates were identified by a growth-inhibition (GI) test (Clyde, 1964), using filter-paper discs impregnated with antisera to both M. gallisepticum and M. synoviae (supplied by the Regional Animal Health Centre, Granada).…”

Mystery swine disease in the Netherlands: The isolation of Lelystad virus

“…Samples were inoculated onto modified, solid and liquid Hayflick media (5), and onto solid and liquid medium I and II (6). Solid media were incubated at 37°C in an atmosphere of 7 per cent CO2; liquid media were incubated at 37°C.…”

Antigenic and genomic homogeneity of successive Mycoplasma hominis isolates

“…The numbers refer to the woman and the letters B, G, S, M and N refer to the relative time of recovery: B, G and S, indicating the first, second and third antenatal hospital visits, respectively; and M and N indicating samples taken from the ear and pharynx, respectively, of the newborn baby. Samples to be analysed for the presence of M. horninis were taken with sterile cotton-tipped swabs and inoculated directly into 1 ml of BE liquid growth medium [12], and frozen at -80°C for later cultivation. Cultures were grown in BEA medium [ …”