Characterisation and Molecular Analysis of an Unusual Chimeric Methicillin Resistant Staphylococcus Aureus Strain and its Bacteriophages

Burgold-Voigt, Sindy; Monecke, Stefan; Simbeck, Alexandra; Holzmann, Thomas; Kieninger, Bärbel; Liebler-Tenorio, Elisabeth M.; Collatz, Maximilian; Diezel, Celia; Müller, Elke; Schneider-Brachert, Wulf; Ehricht, Ralf

doi:10.3389/fgene.2021.723958

Cited by 9 publications

(12 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ciprofloxacin-resistant S. aureus isolates were described soon after the introduction of the agent into clinical practice [ 260 ]. Resistance to fluoroquinolones including ciprofloxacin typically arises as a result of the (i) point mutations in the grlA / grlB genes encoding the subunits of DNA topoisomerase IV and gyrA/gyrB genes encoding the subunits of DNA gyrase [ 33 , 261 ], and (ii) decreased intracellular accumulation and/or active efflux of the drug by membrane-integrated transporter proteins (e.g., NorA) [ 262 ]. Challenging the S. aureus isolates with the ciprofloxacin elevated the norA expression, which potentiates the evolution by increasing the fitness benefit provided by DNA topoisomerase mutations [ 179 ].…”

Section: Mrsa Resistance To Non-β-lactamsmentioning

confidence: 99%

Molecular Basis of Non-β-Lactam Antibiotics Resistance in Staphylococcus aureus

2022

View full text Add to dashboard Cite

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most successful human pathogens with the potential to cause significant morbidity and mortality. MRSA has acquired resistance to almost all β-lactam antibiotics, including the new-generation cephalosporins, and is often also resistant to multiple other antibiotic classes. The expression of penicillin-binding protein 2a (PBP2a) is the primary basis for β-lactams resistance by MRSA, but it is coupled with other resistance mechanisms, conferring resistance to non-β-lactam antibiotics. The multiplicity of resistance mechanisms includes target modification, enzymatic drug inactivation, and decreased antibiotic uptake or efflux. This review highlights the molecular basis of resistance to non-β-lactam antibiotics recommended to treat MRSA infections such as macrolides, lincosamides, aminoglycosides, glycopeptides, oxazolidinones, lipopeptides, and others. A thorough understanding of the molecular and biochemical basis of antibiotic resistance in clinical isolates could help in developing promising therapies and molecular detection methods of antibiotic resistance.

show abstract

Section: Mrsa Resistance To Non-β-lactamsmentioning

confidence: 99%

Molecular Basis of Non-β-Lactam Antibiotics Resistance in Staphylococcus aureus

2022

View full text Add to dashboard Cite

show abstract

“…The differences compared to the reference isolates affect all parts of the genome essentially and are distributed evenly across the genome (with the notable exception of a few highly conserved genes encoding ribosomal proteins). Previous work ( Nimmo et al., 2015 ; Burgold-Voigt et al., 2021 ) showed how a part of the genome of a chimeric isolate genome would match the corresponding part from one parent strain, and differ from the same region of the other one, while this would be conversely for the rest of the genome ( Figure 4 ). Therefore, we can assume that the similarities and differences of the S. roterodami complex compared to S. aureus , S. argenteus and S. schweitzeri do not result from a large-scale chromosomal replacement or chimerism.…”

Section: Discussionmentioning

confidence: 93%

“…200 is integrated into a backbone genome of “green” provenance. These are actually S. aureus CC140 and CC8, in a ST6610 strain, see ( Burgold-Voigt et al., 2021 ). For the Y-axes, different scales were used because differences between the different species are larger than those between different CCs of S. aureus .…”

Section: Resultsmentioning

confidence: 99%

Description of Staphylococcal Strains from Straw-Coloured Fruit Bat (Eidolon helvum) and Diamond Firetail (Stagonopleura guttata) and a Review of their Phylogenetic Relationships to Other Staphylococci

Monecke

Schaumburg

Shittu

et al. 2022

Front. Cell. Infect. Microbiol.

Self Cite

View full text Add to dashboard Cite

The phylogenetic tree of the Staphylococcus aureus complex consists of several distinct clades and the majority of human and veterinary S. aureus isolates form one large clade. In addition, two divergent clades have recently been described as separate species. One was named Staphylococcus argenteus, due to the lack of the “golden” pigment staphyloxanthin. The second one is S. schweitzeri, found in humans and animals from Central and West Africa. In late 2021, two additional species, S. roterodami and S. singaporensis, have been described from clinical samples from Southeast Asia. In the present study, isolates and their genome sequences from wild Straw-coloured fruit bats (Eidolon helvum) and a Diamond firetail (Stagonopleura guttata, an estrildid finch) kept in a German aviary are described. The isolates possessed staphyloxanthin genes and were closer related to S. argenteus and S. schweitzeri than to S. aureus. Phylogenetic analysis revealed that they were nearly identical to both, S. roterodami and S. singaporensis. We propose considering the study isolates, the recently described S. roterodami and S. singaporensis as well as some Chinese strains with MLST profiles stored in the PubMLST database as different clonal complexes within one new species. According to the principle of priority we propose it should be named S. roterodami. This species is more widespread than previously believed, being observed in West Africa, Southeast Asia and Southern China. It has a zoonotic connection to bats and has been shown to be capable of causing skin and soft tissue infections in humans. It is positive for staphyloxanthin, and it could be mis-identified as S. aureus (or S. argenteus) using routine procedures. However, it can be identified based on distinct MLST alleles, and “S. aureus” sequence types ST2470, ST3135, ST3952, ST3960, ST3961, ST3963, ST3965, ST3980, ST4014, ST4075, ST4076, ST4185, ST4326, ST4569, ST6105, ST6106, ST6107, ST6108, ST6109, ST6999 and ST7342 belong to this species.

show abstract

“…Sequence type is obtained based on the alleles identified at each of the seven loci using the SA MLST database. MLST detection of MRSA is based on the sequencing of the seven house-keeping conserved genes in the bacterial chromosome ( 77 ). MLST is also widely used due to its straightforward procedure for characterizing isolates of bacterial species ( 78 ).…”

Section: Discussionmentioning

confidence: 99%

Current molecular approach for diagnosis of MRSA: a meta-narrative review

Sim

Lee

Abushelaibi

et al. 2022

dti

View full text Add to dashboard Cite

Introduction: Detection and diagnosis of MRSA are important in ensuring a correct and effective treatment, further reducing its spread. There are a wide range of molecular approaches for the diagnosis of AMR in MRSA, including PCR, MLST and SCCmec. This review aims to study and appraise current molecular diagnostic methods used for the detection of MRSA. Method: This narrative review was performed by searching the PubMed using the following search terms: (molecular diagnosis) AND (antimicrobial resistance) NOT (review [publication type)) NOT (systematic review [publication type)) NOT (meta-analysis[publication type)) AND (methicillin-resistant staphylococcus aureus). Studies using molecular diagnostic techniques for the detection of MRSA were included, while non-English language, duplicates, and non-article studies were excluded. After reviewing the libraries and a further manual search, 20 studies were included in this paper. RAMESES publication standard for narrative reviews was used for this synthesis. Results: Of the 20 studies included, 11 studies employed PCR for diagnosing MRSA , 2 studies on DNA Microarray, Xpert MRSA/SA BC assay (n=1), MALDI-TOF (n=2), MLST (n=5) and SCCMec typing (n=1). Discussion: Different diagnostic methods used for the diagnosis of MRSA have been studied in this review. Each diagnostic technique have its benefits and drawbacks, however, this study concludes that PCR has been extensively used due to its increased sensitivity and cost-effectiveness.

show abstract

Characterisation and Molecular Analysis of an Unusual Chimeric Methicillin Resistant Staphylococcus Aureus Strain and its Bacteriophages

Cited by 9 publications

References 35 publications

Molecular Basis of Non-β-Lactam Antibiotics Resistance in Staphylococcus aureus

Molecular Basis of Non-β-Lactam Antibiotics Resistance in Staphylococcus aureus

Description of Staphylococcal Strains from Straw-Coloured Fruit Bat (Eidolon helvum) and Diamond Firetail (Stagonopleura guttata) and a Review of their Phylogenetic Relationships to Other Staphylococci

Current molecular approach for diagnosis of MRSA: a meta-narrative review

Contact Info

Product

Resources

About