Characterisation, dissemination and persistence of gentamicin resistant Escherichia coli from a Danish university hospital to the waste water environment

Jakobsen, Lotte; Sandvang, Dorthe; Hansen, Lars H.; Bagger-Skjøt, Line; Westh, Henrik; Jørgensen, Claus; Hansen, Dennis Schrøder; Pedersen, B.; Monnet, Dominique; Frimodt‐Møller, Niels; Sørensen, Søren J.; Hammerum, Anette M.

doi:10.1016/j.envint.2007.07.011

Cited by 62 publications

(42 citation statements)

References 21 publications

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“…In our study, a high prevalence of the aac(3)-IIa gene was observed in the aminoglycosideresistant strains (71.83%), with several other studies also reporting a high prevalence of this gene (20,21).…”

Section: Discussionsupporting

confidence: 49%

Plasmid Profile Analysis of Aminoglycoside-Resistant Escherichia coli Isolated From Urinary Tract Infections

Soleimani¹,

Derakhshan²,

Memariani³

2016

Int J Enteric Pathog

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Background: Uropathogenic E. coli (UPEC) is the primary cause of human urinary tract infections (UTIs) worldwide. Moreover, there has been renewed and growing interest in using older antibiotics for treatment, such as aminoglycosides. Objectives: The goal of this study was to determine the plasmid profile patterns of UPEC isolates harboring the aminoglycoside resistance gene aac(3)-IIa. Patients and Methods: A total of 276 uropathogenic E. coli (UPEC) samples were isolated from UTI patients at the Tehran heart center in Tehran, Iran. Antimicrobial susceptibility testing against five aminoglycosides was performed by the disk diffusion method, and the aac(3)-IIa gene was detected via PCR. Moreover, plasmid profiling was carried out on those UPEC isolates harboring the aac(3)-IIa gene. Finally, the similarities among these isolates were determined on the basis of their plasmid profiles. Results: The highest level of resistance was seen for tobramycin (24.6%), and the aac(3)-IIa gene was found in 51 isolates. Twentyseven different plasmid profiles were identified among the isolates harboring the aac(3)-IIa gene, with the 15 kb plasmid being the most common. Moreover, no significant correlation was found between the resistance patterns and the number of plasmids. The cluster analysis based on the plasmid profiles grouped the isolates into five different clusters, of which cluster one was the largest (containing 14 of 51 isolates). Conclusions: Our data suggest the monitoring of aminoglycoside resistance, and its consideration in the empirical therapy of UPEC infections.

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Section: Discussionsupporting

confidence: 49%

Plasmid Profile Analysis of Aminoglycoside-Resistant Escherichia coli Isolated From Urinary Tract Infections

Soleimani¹,

Derakhshan²,

Memariani³

2016

Int J Enteric Pathog

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“…Our results allow a fundamental step forward in the understanding of the full virulence potential and, to a lesser extent, of the antimicrobial resistance potential of UTI isolates as well as commensal animal and food isolates. UTI, animal and meat E. coli isolates have been investigated previously for their virulence genes but never to this extent, and only a few isolates were investigated simultaneously for antimicrobial resistance genes (Bonnet et al, 2009;Ewers et al, 2007Ewers et al, , 2009Hamelin et al, 2006Hamelin et al, , 2007Hannah et al, 2009;Jakobsen et al, 2008;Johnson et al, 2005bJohnson et al, , 2009Maynard et al, 2004 The extensive gene profiling, epidemiologically sampled isolates, analysis of B2 isolates only and the many isolate origins investigated here make up the strength of this study and provide radical difference from previous studies. Interestingly, despite this extensive gene profiling, similarities between isolates from UTI, animal and meat E. coli was clearly apparent.…”

Section: Discussionmentioning

confidence: 75%

Microarray-based detection of extended virulence and antimicrobial resistance gene profiles in phylogroup B2 Escherichia coli of human, meat and animal origin

Jakobsen

Garneau

Kurbasic

et al. 2011

Journal of Medical Microbiology

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Extra-intestinal pathogenic Escherichia coli (ExPEC) causing urinary tract infections (UTIs) most often belong to phylogenetic group B2 and stem from the patient's own faecal flora. It has been hypothesized that the external reservoir for these uropathogenic E. coli in the human intestine may be meat and food-production animals. To investigate such a connection, this study analysed an E. coli phylogroup B2 strain collection (n5161) of geographical and temporally matched isolates, published previously, from UTI patients (n552), community-dwelling humans (n536), imported (n55) and Danish (n513) broiler chicken meat, Danish broiler chickens (n517), imported (n53) and Danish (n527) pork, and healthy Danish pigs (n58). The isolates were subjected to microarray analysis for 315 virulence genes and variants and 82 antimicrobial resistance genes and variants. In total, 133 different virulence and antimicrobial resistance genes were detected in at least one UTI isolate. Between 66 and 87 of these genes were also detected in meat and animal isolates. Cluster analyses of virulence and resistance gene profiles, respectively, showed that UTI and community-dwelling human isolates most often grouped with meat and animal isolates, indicating genotypic similarity among such isolates. Furthermore, B2 isolates were detected from UTI patients and meat, with indistinguishable gene profiles. A considerable proportion of the animal and meat isolates belonged to the ExPEC pathotype. In conclusion, these findings suggest that B2 E. coli from meat and animal origin can be the source of most of the virulence and antimicrobial resistance genes detected in uropathogenic E. coli isolates and that there is a general resemblance of animal, meat and UTI E. coli based on extended gene profiling. These findings support the hypothesis of a zoonotic link between E. coli causing UTIs and E. coli from meat and animals.3Dr Brousseau passed away before the completion of the study.4Present address: Steno Diabetes Center A/S, Niels Steensens

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“…Intake of trans fatty acids has been associated with an increased risk of human cardiovascular disease but results from epidemiological studies have indicated that the intake of rumenic trans FA is innocuous or even protects against cardiovascular disease (Jakobsen et al, 2008;Bendsen et al, 2011). In the industrially produced hydrogenated fats, the elaidic acid and trans-10 18:1 are the prevailing trans-FA while VA is the major trans-FA in milk.…”

Section: Discussionmentioning

confidence: 99%

Milk fatty acid profile from grazing buffaloes fed a blend of soybean and linseed oils

Gagliostro

Patiño²,

Negrette³

et al. 2015

Arq. Bras. Med. Vet. Zootec.

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The aim of the study was to examine the changes in milk fatty acid (FA) profile of grazing buffaloes fed either low (L, 276g/d) or high (H, 572g/d) doses of a blend (70:30, wt/wt) of soybean and linseed oils. Fourteen multiparous Mediterranean buffaloes grazing on a native pasture were fed 4 kg/day of a commercial concentrate containing no supplemental oil over a pre-experimental period of ten days. The baseline milk production and composition and milk FA profile were measured over the last three days. After this pre-experimental period the animals received the same concentrate added with either the L or H oil doses for 26 additional days. Milk yield (g/animal/day) did not differ at the start (1776 ± 522 and 1662 ± 291 for L and H, respectively, P<0.622) or at the end of the trial (4590 ± 991 and 4847 ± 447 in L and H, respectively, P<0.543). Baseline milk fat content (g/kg) averaged 77.1 (±20.5) in L and 74.3 (±9.9) in H (P<0.10) and was reduced (P<0.031) to 60.7 (±23.6) and 49.4 (±11.2) (P<0.0031) respectively after L and H with no differences between treatments (P<0.277). Baseline milk protein content (L=43.2 ± 3.4 and H= 44.3 ± 6.9g/kg) increased after oil supplementation (P<0.0001) in both L (73.2 ± 6.0g/kg) and H (68.4 ± 4.9g/kg) without differences between oil doses (P<0.123). Milk fat content of 14:0 decreased after oil supplementation only in the H treatment (5.29 to 4.03, P<0.007) whereas that of 16:0 was reduced (P<0.001) at both L (24.49 to 19.75g/100g FA) and H (25.92 to 19.17g/100g FA) doses. The reduction of total content of 12:0 to 16:0 was higher (P<0.052) in H (32.02 to 23.93g/100g FA) than L (30.17 to 25.45g/100g FA). Vaccenic acid content increased (P<0.001) from 5.70 to 13.24g/100g FA in L and from 5.25 to 16.77 in H, with higher results in the in H treatment (P<0.001). Baseline rumenic acid was sharply increased (P<0.001) in L (1.80 to 4.09g/100g FA, +127%) and H (1.60 to 4.61g/100g FA, +187%) with no differences between L and H (P<0.19). Overall, these results indicate a pronounced improvement in the nutritional value of milk fat from grazing buffaloes fed little amounts (0.276g/day) of a blend of soybean and linseed oils.

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Characterisation, dissemination and persistence of gentamicin resistant Escherichia coli from a Danish university hospital to the waste water environment

Cited by 62 publications

References 21 publications

Plasmid Profile Analysis of Aminoglycoside-Resistant Escherichia coli Isolated From Urinary Tract Infections

Plasmid Profile Analysis of Aminoglycoside-Resistant Escherichia coli Isolated From Urinary Tract Infections

Microarray-based detection of extended virulence and antimicrobial resistance gene profiles in phylogroup B2 Escherichia coli of human, meat and animal origin

Milk fatty acid profile from grazing buffaloes fed a blend of soybean and linseed oils

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