Characterisation of 13 glutamate receptor-like genes encoded in the tomato genome by structure, phylogeny and expression profiles

Aouini, Asma; Matsukura, Chiaki; Ezura, Hiroshi; Asamizu, Erika

doi:10.1016/j.gene.2011.11.037

Cited by 34 publications

(44 citation statements)

References 41 publications

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“…However, their role in the physiology and development of plants remains unclear. Recently, a family of 13 tomato GLR genes was identi ed (Aouini et al 2012). To investigate the physiological roles of the SlGLR genes, we generated transgenic lines of Arabidopsis overexpressing SlGLR1.1 and SlGLR3.5.…”

Section: Discussionmentioning

confidence: 99%

“…In animals, iGluRs are ligand-gated non-selective cation channels that allow the flow of Na + and K + and sometimes Ca 2+ in response to glutamate binding (Tabassum and Feroz 2011 Phylogenetic analyses in tomato enabled the classification of GLR family genes into three clades (Aouini et al 2012). SlGLR3.5 was included in clade III and SlGLR1.1 was included in clade I. e classi cation of SlGLRs revealed the divergence of a distinct Clade I represented by two genes (SlGLR1.1 and SlGLR1.2) and indicated that this clade is possibly speci c to the Solanaceae family.…”

Section: Discussionmentioning

confidence: 99%

“…Although SlGLR1.1 and SlGLR3.5 are expressed in di erent tissues and classi ed into distinct clades, they appear to play similar roles in Ca 2+ homeostasis and signaling. Kang et al (2006) and monovalent cation transport and form constitutively active ion channels as re ected by their protein sequence (Aouini et al 2012). Furthermore, using a domain swapping approach and heterologous expression in Xenopus oocytes, an analysis of chimeric proteins that contain the ion pore domains of each of 17 Arabidopsis GLR proteins in either rat GluR1 or GluR6 receptors suggested that AtGLR1.1 and AtGLR1.4 have functional Na + , K + and Ca 2+ permeable pore domains, and that currents through the AtGLR1.1 pore resemble the glutamate-activated currents observed in plants (Tapken and Hollmann 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Both lines also displayed a decreased number of seeds per silique, suggesting partial male sterility. SlGLR1.1, which is expressed in tomato roots (Aouini et al 2012), may be a good candidate for studying the role of GLRs in the root tip. Li et al (2006) revealed that the short-root phenotype of a rice mutant defective in the expression of the OsGLR3.1 gene is due to the disruption of meristematic activity in the root apex, suggesting that this GLR gene plays an essential role in the maintenance of the root meristem.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, a new family of 13 SlGLR genes was identi ed in the tomato, Solanum lycopersicum (Aouini et al 2012). …”

Section: Based On the Arabidopsis Genome Sequence Data 20mentioning

confidence: 99%

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Overexpression of the tomato glutamate receptor-like genes SlGLR1.1 and SlGLR3.5 hinders Ca2+ utilization and promotes hypersensitivity to Na+ and K+ stresses

Aouini

Hernould

Ariizumi

et al. 2012

Plant Biotechnology

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Recently, a new family of 13 SlGLR genes was identi ed in the tomato, Solanum lycopersicum (Aouini et al 2012). …”

Section: Based On the Arabidopsis Genome Sequence Data 20mentioning

confidence: 99%

See 3 more Smart Citations

Overexpression of the tomato glutamate receptor-like genes SlGLR1.1 and SlGLR3.5 hinders Ca2+ utilization and promotes hypersensitivity to Na+ and K+ stresses

Aouini

Hernould

Ariizumi

et al. 2012

Plant Biotechnology

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A Single‐Nucleotide Mutation in a GLUTAMATE RECEPTOR‐LIKE Gene Confers Resistance to Fusarium Wilt in Gossypium hirsutum

et al. 2021

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Fusarium wilt (FW) disease of cotton, caused by the fungus Fusarium oxysporum f. sp. vasinfectum (Fov), causes severe losses in cotton production worldwide. Though significant advancements have been made in development of FW‐resistant Upland cotton (Gossypium hirsutum) in resistance screening programs, the precise resistance genes and the corresponding molecular mechanisms for resistance to Fov remain unclear. Herein it is reported that Fov7, a gene unlike canonical plant disease‐resistance (R) genes, putatively encoding a GLUTAMATE RECEPTOR‐LIKE (GLR) protein, confers resistance to Fov race 7 in Upland cotton. A single nucleotide polymorphism (SNP) (C/A) in GhGLR4.8, resulting in an amino acid change (L/I), is associated with Fov resistance. A PCR‐based DNA marker (GhGLR4.8SNP(A/C)) is developed and shown to cosegregate with the Fov resistance. CRISPR/Cas9‐mediated knockout of Fov7 results in cotton lines extremely susceptible to Fov race 7 with a loss of the ability to induce calcium influx in response to total secreted proteins (SEPs) of Fov. Furthermore, coinfiltration of SEPs with GhGLR4.8A results in a hypersensitive response. This first report of a GLR‐encoding gene that functions as an R gene provides a new insight into plant–pathogen interactions and a new handle to develop cotton cultivars with resistance to Fov race 7.

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Application of T-DNA activation tagging to identify glutamate receptor-like genes that enhance drought tolerance in plants

Lü¹,

Wang²,

Liu³

et al. 2014

Plant Cell Rep

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A high-quality rice activation tagging population has been developed and screened for drought-tolerant lines using various water stress assays. One drought-tolerant line activated two rice glutamate receptor-like genes. Transgenic overexpression of the rice glutamate receptor-like genes conferred drought tolerance to rice and Arabidopsis. Rice (Oryza sativa) is a multi-billion dollar crop grown in more than one hundred countries, as well as a useful functional genetic tool for trait discovery. We have developed a population of more than 200,000 activation-tagged rice lines for use in forward genetic screens to identify genes that improve drought tolerance and other traits that improve yield and agronomic productivity. The population has an expected coverage of more than 90 % of rice genes. About 80 % of the lines have a single T-DNA insertion locus and this molecular feature simplifies gene identification. One of the lines identified in our screens, AH01486, exhibits improved drought tolerance. The AH01486 T-DNA locus is located in a region with two glutamate receptor-like genes. Constitutive overexpression of either glutamate receptor-like gene significantly enhances the drought tolerance of rice and Arabidopsis, thus revealing a novel function of this important gene family in plant biology.

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Characterisation of 13 glutamate receptor-like genes encoded in the tomato genome by structure, phylogeny and expression profiles

Cited by 34 publications

References 41 publications

Overexpression of the tomato glutamate receptor-like genes SlGLR1.1 and SlGLR3.5 hinders Ca2+ utilization and promotes hypersensitivity to Na+ and K+ stresses

Overexpression of the tomato glutamate receptor-like genes SlGLR1.1 and SlGLR3.5 hinders Ca2+ utilization and promotes hypersensitivity to Na+ and K+ stresses

A Single‐Nucleotide Mutation in a GLUTAMATE RECEPTOR‐LIKE Gene Confers Resistance to Fusarium Wilt in Gossypium hirsutum

Application of T-DNA activation tagging to identify glutamate receptor-like genes that enhance drought tolerance in plants

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