Characterisation of a human homologue of a yeast cell division cycle gene, MCM6, located adjacent to the 5′ end of the lactase gene on chromosome 2q21

HsMCM6: a new member of the human MCM/P1 family encodes a protein homologous to fission yeast Mis5

“…We observed no other reproducible signal. This location coincides with the previous report, in which HsMCM6 was identified 3.3 kb upstream of the intestinal lactase gene on chromosome 2q21 (Harvey et al 1996). HsMCM6 localized at a distinct site from those of HsMCM2 at 3q21 (Mincheva et al 1994), HsMCM7 at 7q21 (Nakatsuru et al 1995;Endo et al unpublished data) and HsMCM3 at 6p12 (Starborg et al 1995) identical amounts of RNA per agarose gel, transferred the RNA to nylon membranes and investigated the mRNA levels for HsMCM2, -3, -4, -5 and -7 genes using the DNA fragments of their corresponding cDNAs as probes.…”

Transcriptional regulation of the lactase-phlorizin hydrolase gene by polymorphisms associated with adult-type hypolactasia

“…25 However, RT-PCR experiments have shown that unlike LPH, expression of MCM6 is not restricted in mucosa and there is no individual variation in adult intestine. 26 In conclusion, RT-PCR followed by solid phase minisequencing, allowing accurate determination of relative mRNA levels of LPH transcripts, appeared to find an order of magnitude higher expression of the persistent allele in subjects heterozygous for both the CT −13910 and GA −22018 persistent genotypes. The effect of the T −13910 , A −22018 allele was also observed as elevation of the L/S ratio.…”

LCT 13910 C/T polymorphism, serum calcium, and bone mineral density in postmenopausal women

“…To perform the SNP genotyping, we used TaqMan probes and primers [15]. LCT 13910 C/T (rs4988235) polymorphism with thymine (T)-cytosine (C) nucleotide alteration is located in the initial codon of LCT, at intron 13 of MCM6 [16]. It was genotyped with the following sequence of allele specific probes: ATA AGA TAA TGT AGC CCC TGG C, ATA AGA TAA TGT AGT CCC TGG C, and primers 5′-CTC TGC GCT GGC AAT ACA G and 5′-AAA TGC AAC CTA AGG AGG AGA.…”