Characterisation of a membrane receptor on ruminants and equine platelets and peripheral blood leukocytes similar to the human integrin receptor glycoprotein ()

Pintado, Carlos; Friend, M.; Llanes, D.

doi:10.1016/0165-2427(94)05310-o

Cited by 22 publications

(11 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…GPIIb/IIIa is a specific megakaryocyte/platelet marker and many investigators have examined its function on platelets (Pintado et al , 1995; Do Ceu Monteiro et al , 1999). GPIIb/IIIa plays an important role in the aggregation of platelets isolated from peripheral blood (Plow et al , 1985; Plow & Ginsberg, 1989).…”

Section: Discussionmentioning

confidence: 99%

Development of a liquid culture system for megakaryocyte terminal differentiation: fibrinogen promotes megakaryocytopoiesis but not thrombopoiesis

et al. 2003

View full text Add to dashboard Cite

Summary. Megakaryocyte differentiation is composed of three distinct stages: formation of erythromegakaryocytic progenitor cells, maturation of megakaryocytes and production of platelets. We have developed a liquid culture system for megakaryocyte terminal differentiation from haematopoietic stem cells into proplatelets. In this system, CD34 + cells isolated from human cord blood, differentiated to CD41 + cells, were classified either as propidium iodide (PI) + cells (large) or PI -cells (small) by fluorescence-activated cell sorting analysis on the late-stage CD41 + cells. Transmission electron microscopy showed that the cultured small cells were morphologically identical to platelets isolated from normal peripheral blood. Moreover, the number of differentiated cells that were CD42b-positive attained an approximately 60-fold expansion over that of the primary CD34 + cells in this culture system. Furthermore, gene expression of megakaryocytopoietic transcriptional factors, GATA-1 and NF-E2, and several megakaryocytic markers such as glycoprotein (GP)IIb and thromboxane synthase was observed in the individual differentiation stage. Treatment with fibrinogen, a ligand of GPIIb/IIIa, increased the number of CD41 + /PI + cells, but treatment in the late stage suppressed CD41 + /PI -cell formation, suggesting that fibrinogen promotes megakaryocytopoiesis, but not thrombopoiesis. We conclude that this liquid culture system using human CD34 + cells may be used to mimic the physiological development from haematopoietic stem cells into megakaryocytes, as well as promote subsequent thrombopoiesis.

show abstract

Section: Discussionmentioning

confidence: 99%

Development of a liquid culture system for megakaryocyte terminal differentiation: fibrinogen promotes megakaryocytopoiesis but not thrombopoiesis

et al. 2003

View full text Add to dashboard Cite

show abstract

“…This deficiency results in an impaired platelet aggregation and clot retraction, platelet transfusion being the only specific therapy. 10 Our group has developed different antibodies specific for CD41/CD61 in swine and ruminants, 11,12 and one of these (JM2E5), that was able to react to the CD61 molecule of many species beside swine, 11 has been employed in this work. In this paper we have used the mAb JM2E5 to study of the expression of the CD61 antigen on canine cells; in particular, to determine whether the CD61 molecule is present as CD41/ CD61 or CD51/CD61 in peripheral blood cells and platelets.…”

Section: Introductionmentioning

confidence: 99%

Expression of CD61 (β₃integrin subunit) on canine cells

et al. 2001

Self Cite

View full text Add to dashboard Cite

A monoclonal antibody (JM2E5) specific for the integrin beta3 chain, or CD61 or GPIIIa subunit, has been employed to determine the expression of the canine homologue CD41/CD61 or CD51/CD61 complex on different canine cells in peripheral blood lymphocytes, monocytes, granulocytes, platelets, erythrocytes, lymph-node cells, spleen cells and breast tumour cells). The canine homologue CD41/CD61 or CD51/61 was present on peripheral blood lymphocytes, monocytes, granulocytes, breast tumour cells and spleen cells as well as on platelets and it was absent from erythrocytes and lymph-node cells. An antigen with components of molecular masses of 25/100/120 kDa (under reducing conditions) was immunoprecipitated from canine peripheral lymphocytes and platelets, but not from granulocytes or monocytes. Expression on canine lymphocytes of the canine homologue of the human beta3 integrin chain was unexpected, based on the expression pattern of this molecule in human tissue.

show abstract

“…The first stage of megakaryocyte development is the differentiation and proliferation of hematopoietic stem cells into bipotential erythroid/megakaryocytic cells and then into megakaryoblasts. The second stage involves nuclear polyploidization, demarcation membrane formation, cell size increase, and the expression of specific surface markers (CD41, CD61, or CD42b) [20], [21]. Megakaryocytes become polyploid cells through repeated DNA replication cycles without cytoplasmic division.…”

Section: Introductionmentioning

confidence: 99%

Suppressive Effects of Anthrax Lethal Toxin on Megakaryopoiesis

et al. 2013

View full text Add to dashboard Cite

Anthrax lethal toxin (LT) is a major virulence factor of Bacillus anthracis. LT challenge suppresses platelet counts and platelet function in mice, however, the mechanism responsible for thrombocytopenia remains unclear. LT inhibits cellular mitogen-activated protein kinases (MAPKs), which are vital pathways responsible for cell survival, differentiation, and maturation. One of the MAPKs, the MEK1/2-extracellular signal-regulated kinase pathway, is particularly important in megakaryopoiesis. This study evaluates the hypothesis that LT may suppress the progenitor cells of platelets, thereby inducing thrombocytopenic responses. Using cord blood-derived CD34+ cells and mouse bone marrow mononuclear cells to perform in vitro differentiation, this work shows that LT suppresses megakaryopoiesis by reducing the survival of megakaryocytes. Thrombopoietin treatments can reduce thrombocytopenia, megakaryocytic suppression, and the quick onset of lethality in LT-challenged mice. These results suggest that megakaryocytic suppression is one of the mechanisms by which LT induces thrombocytopenia. These findings may provide new insights for developing feasible approaches against anthrax.

show abstract

Characterisation of a membrane receptor on ruminants and equine platelets and peripheral blood leukocytes similar to the human integrin receptor glycoprotein ()

Cited by 22 publications

References 9 publications

Development of a liquid culture system for megakaryocyte terminal differentiation: fibrinogen promotes megakaryocytopoiesis but not thrombopoiesis

Development of a liquid culture system for megakaryocyte terminal differentiation: fibrinogen promotes megakaryocytopoiesis but not thrombopoiesis

Expression of CD61 (β₃integrin subunit) on canine cells

Suppressive Effects of Anthrax Lethal Toxin on Megakaryopoiesis

Contact Info

Product

Resources

About

Characterisation of a membrane receptor on ruminants and equine platelets and peripheral blood leukocytes similar to the human integrin receptor glycoprotein ()

Cited by 22 publications

References 9 publications

Development of a liquid culture system for megakaryocyte terminal differentiation: fibrinogen promotes megakaryocytopoiesis but not thrombopoiesis

Development of a liquid culture system for megakaryocyte terminal differentiation: fibrinogen promotes megakaryocytopoiesis but not thrombopoiesis

Expression of CD61 (β3integrin subunit) on canine cells

Suppressive Effects of Anthrax Lethal Toxin on Megakaryopoiesis

Contact Info

Product

Resources

About

Expression of CD61 (β₃integrin subunit) on canine cells