Characterisation of a repetitive DNA sequence from the malaria parasite, Plasmodium falciparum

Oquendo, P.; Goman, Michael; Mackay, Martin; Langsley, Gordon; Walliker, David; Scaife, John

doi:10.1016/0166-6851(86)90053-8

Cited by 96 publications

(54 citation statements)

References 35 publications

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“…The availability of DNA derived techniques for diagnosis of infectious agents with high specificity and sensitivity has represented an attractive alternative. Several groups have identified species specific repetitive sequences in the genome of P. falciparum (Franzen et al 1984, Oquendo et al 1986, Barker et al 1986, McLaughlin et al 1987) and derived DNA probes in an attempt to improve diagnosis. The highly repeated subtelomeric rep-20 sequence has been target of DNA probes as well as PCR diagnostic assays (Barker al.…”

Section: Discussionmentioning

confidence: 99%

“…Primers selection and PCR assays -Two oligonucleotides were designed: (1) a primer of 16 bp, pf-16 (5'-ACT AAC TTA GGT CTTA-3'), and (2) a primer of 21bp pf-21 (5'-ATG TTA GTC AAC TTA AGA CCT-3'), both derived from the pf-21 consensus sequence reported by Oquendo et al (1986).…”

Section: Methodsmentioning

confidence: 99%

“…One of these consists of 21-bp blocks, imperfectly repeated in tandem clusters oriented in opposite directions (rep-20). These rep-20 sequences are found in all chromosomes of the parasite (Oquendo et al 1986). DNA probes directed to these repeats hybridize with P. falciparum strains from South America, Africa, and South and Southeast Asia (Buesing et al 1987), and their sensitivity is comparable to that of conventional microscopy (Lanar et al 1989).…”

mentioning

confidence: 99%

“…Assay strategies have been proposed that directly detect abundant parasite nucleic acid sequences, including repetitive DNA (Franzen et al 1984, Barker et al 1986, Oquendo et al 1986, McLaughlin et al 1987, Zolg et al 1987 or ribosomal RNA (rRNA) (Lal et al 1989, Wathers & McCutchan 1989). …”

mentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of DNA Recombinant Methodologies for the Diagnosis of Plasmodium falciparum and their Comparison with the Microscopy Assay

Urdaneta¹,

Guevara²,

Jl³

1998

Mem. Inst. Oswaldo Cruz

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Evaluation of DNA Recombinant Methodologies for the Diagnosis of Plasmodium falciparum and their Comparison with the Microscopy Assay

Urdaneta¹,

Guevara²,

Jl³

1998

Mem. Inst. Oswaldo Cruz

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“…Esta organización muestra una localización conservada entre PFCOL692 y pRep20, lo cual es muy interesante puesto que se sabe que pRep20 está presente en el subtelómero de todos los cromosomas de F! falciparum, ocupando regiones extensas (1 % del genoma aproximadamente) y que tiene una localización relativamente conservada cerca al telómero (5,17,18,30).…”

Section: Posible Ubicación De Pfcol692 En El Subtelómerounclassified

Ubicación de la secuencia repetitiva PFCOL692 en fragmentos genómicos de Plasmodíum falciparum

Alvarado¹,

Rojas²,

Guerrero³

et al. 2000

biomedica

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ResumenLas secuencias repetitivas son componentes estructurales de los genomas eucariotes. Se desconoce la función de la mayoría de ellas, pero, al parecer, no son simplemente ADN egoísta sino que intervienen en procesos de recombinación y regulación génica. En este estudio se estableció la localización subtelomérica d e l a secuencia repetitiva PFCOL692 en los cromosomas de Plasmodium falciparum, a través de la caracterización de cuatro clones de la genoteca hEMBL4lPFCOL692, que contenían insertos entre 15 y 23Kb de ADN genómico del parásito; esta caracterización se hizo mediante análisis de restricción y la posible ubicación de PFCOL692 en el genoma se exploró utilizando sondas específicas para las regiones telomérica (pTB4.1) y subtelomérica (pRep20) de los cromosomas de P falciparum. El análisis de los mapas de restricción obtenidos permitió plantear una posible ubicación de PFCOL692 en el extremo de los cromosomas del parásito, donde las copias de esta secuencia se encuentran agrupadas en un segmento del subtelómero y con una posición ~0 n s e~a d a con respecto a la secuencia pRep20 Se sugiere, además, que PFCOL692 no se encuentra en el limite entre el subtelómero y el telómero.Palabras clave: Plasmodium falciparum, secuencias repetitivas, región subtelomérica Location o f the repetitive sequence PFCOL692 in genomic fragments of Plasmodium falciparum AbstractIn this paper we established the subtelomeric localization of the repetitive sequence PFCOL692 of Plasmodium falciparum by the characterization of four clones from a hEMBL41 PFCOL692 genomic library, which contains 15-23Kbp long inserts of parasite genomic DNA. Characterization was made by restriction analysis and the PFCOL692 localization in the genome was explored using specific probes for telomeric (pTB4.1) and subtelomeric regions (pRep20) of P falciparum chromosomes. We proposed a possible organization for the parasite chromosomes ends, where the copies of PFCOL692 are clusters in the subtelomeric region and their localization is conserved with respect to the pRep20 sequence. In addition, we established that PFCOL692 is not located next to the telomere.

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A malaria protein exported into a new compartment within the host erythrocyte.

Simmons¹,

Woollett²,

Bergin-Cartwright³

et al. 1987

The EMBO Journal

124

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A Plasmodium falciparum protein which is exported into a new compartment in the host erythrocyte has been located. This protein, exp-1, has a variable region recognized by a monoclonal antibody. Naturally occurring mutants of this region have been characterized. All mutants studied so far have the same A -G transition abolishing the target for the antibody. The exp-1 gene has a complex structure containing two introns. It is highly conserved in five independent, genetically defined parasite lines, suggesting that exp-1 has an important function. We discuss the possible role of exp-1 in P. falciparum infections.

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Characterisation of a repetitive DNA sequence from the malaria parasite, Plasmodium falciparum

Cited by 96 publications

References 35 publications

Evaluation of DNA Recombinant Methodologies for the Diagnosis of Plasmodium falciparum and their Comparison with the Microscopy Assay

Evaluation of DNA Recombinant Methodologies for the Diagnosis of Plasmodium falciparum and their Comparison with the Microscopy Assay

Ubicación de la secuencia repetitiva PFCOL692 en fragmentos genómicos de Plasmodíum falciparum

A malaria protein exported into a new compartment within the host erythrocyte.

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