Characterisation of an antinematicidal compound from<i>Leucaena leucocephala</i>

Adekunle, O. K.; Aderogba, M.A.

doi:10.1071/dn08066

Cited by 9 publications

(4 citation statements)

References 9 publications

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“…The egg-laying activity in this study was also found to be significantly reduced after exposure to LL extract and mimosine, as demonstrated by the decrease in the mean number of eggs laid. Similar research findings concordantly showed the inhibition of egg-laying and -hatching post-treatment with LL extracts in different nematode species [ 30 , 32 , 74 , 75 , 76 , 77 , 78 , 79 , 80 ]. For instance, a recent study by Romero et al (2020) [ 76 ] reported that the ethanolic extract of LL leaves significantly inhibited egg-hatching activity up to 54% in Haemonchus contortus.…”

Section: Discussionsupporting

confidence: 82%

Anthelmintic Effect of Leucaena leucocephala Extract and Its Active Compound, Mimosine, on Vital Behavioral Activities in Caenorhabditis elegans

2022

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Helminth infections continue to be a neglected global threat in tropical regions, and there have been growing cases of anthelmintic resistance reported towards the existing anthelmintic drugs. Thus, the search for a novel anthelmintic agent has been increasing, especially those derived from plants. Leucaena leucocephala (LL) is a leguminous plant that is known to have several pharmacological activities, including anthelmintic activity. It is widely known to contain a toxic compound called mimosine, which we believed could be a potential lead candidate that could exert a potent anthelmintic effect. Hence, this study aimed to validate the presence of mimosine in LL extract and to investigate the anthelmintic effect of LL extract and mimosine on head thrashing, egg-laying, and pharyngeal pumping activities using the animal model Caenorhabditis elegans (C. elegans). Mimosine content in LL extract was confirmed through an HPLC analysis of spiking LL extract with different mimosine concentrations, whereby an increasing trend in peak heights was observed at a retention time of 0.9 min. LL extract and mimosine caused a significant dose-dependent increase in the percentage of worm mortality, which produced LC50s of 73 mg/mL and 6.39 mg/mL, respectively. Exposure of C. elegans to different concentrations of LL extract and mimosine significantly decreased the head thrashing, egg-laying, and mean pump amplitude of pharyngeal pumping activity. We speculated that these behavioral changes are due to the inhibitory effect of LL extract and mimosine on an L-type calcium channel called EGL-19. Our findings provide evidential support for the potential of LL extract and its active compound, mimosine, as novel anthelmintic candidates. However, the underlying mechanism of the anthelmintic action has yet to be elucidated.

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Section: Discussionsupporting

confidence: 82%

Anthelmintic Effect of Leucaena leucocephala Extract and Its Active Compound, Mimosine, on Vital Behavioral Activities in Caenorhabditis elegans

2022

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“…The activity observed was substantiated by the presence of secondary metabolites in the extract of L. leucocephala. The nematicidal activity of quercertin a phenolic compound isolated from L. leucocephala was reiterated by Adekunle and Aderogba (2008). They reported a high level of toxicity to M. incognita eggs.…”

Section: Discussionmentioning

confidence: 99%

Environmental Sustainability: Bioactivity of Leucaena leucocephala Leaves and Pesticide Residue Analysis in Tomato Fruits

Fabiyi¹,

Olatunji²

2021

Acta Univ. Agric. Silvic. Mendelianae Brun.

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Field studies were conducted on the application of Leucaena leucocephala leaf extracts as an alternative to carbofuran a synthetic nematicide, while possible residues of carbofuran in tomato fruits were also analysed. Acetone, methanol and petroleum ether fractions of the leaves of L. leucocephala were applied at 200 mg/mL in variants of 100 mL, 150 mL and 200 mL to a naturally nematode infested field. Comparison was made with carbofuran a synthetic insecticide and nematicide. Samples of tomato fruits from carbofuran treated and untreated beds were extracted with ethyl acetate for residual pesticide analysis to ascertain the exceedance of the maximum residue limit (MRL). Results revealed that pesticide residue was significantly (p = 0.05) higher in plots treated with carbofuran compared with standard MRL for carbofuran in tomatoes. Crude extracts of L. leucocephala were as effective as carbofuran, while the fraction was however significantly better in producing higher numbers of fruits and reduced nematode population in root and soil of tomato plants.

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“…In field trials, crude extracts of Tephrosia purpurea were shown to significantly reduce root-galling in French bean ( Njenga et al, 2019 ); while extracts from T. cinerea stem were bioactive against M. incognita ( Cristóbal-Alejo et al, 2006 ). Additionally, extracts of roots and leaves of Leucaena leucocephala have been reported to contain flavonoids like quercetin known to be highly toxic to M. incognita ( Adekunle and Aderogba, 2008 ); Medicago sativa is also known to contain saponin mixture, with nematicidal potency reported against X. index , M. incognita , and G. rostochiensis ; and when amended in soil, significant population reductions were evident ( D’Addabbo et al, 2011 ).…”

Section: Composition Activity and Potency Of The Active Constituentsmentioning

confidence: 99%

A Review of the Potency of Plant Extracts and Compounds from Key Families as an Alternative to Synthetic Nematicides: History, Efficacy, and Current Developments

Mwamula

Kabir²,

Lee

2022

Plant Pathol J

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The global nematicides market is expected to continue growing. With an increasing demand for synthetic chemical-free organic foods, botanical nematicides are taking the lead as replacements. Consequently, in the recent years, there have been vigorous efforts towards identification of the active secondary metabolites from various plants. These include mostly glucosinolates and their hydrolysis products such as isothiocyanates; flavonoids, alkaloids, limonoids, quassinoids, saponins, and the more recently probed essential oils, among others. And despite their overwhelming potential, variabilities in quality, efficacy, potency and composition continue to persist, and commercialization of new botanical nematicides is still lagging. Herein, we have reviewed the history of botanical nematicides and regional progresses, the potency of the identified phytochemicals from the key important plant families, and deciphered some of the impediments involved in standardization of the active compounds in addition to the concerns over the safety of the purified compounds to non-target microbial communities.

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Characterisation of an antinematicidal compound fromLeucaena leucocephala

Cited by 9 publications

References 9 publications

Anthelmintic Effect of Leucaena leucocephala Extract and Its Active Compound, Mimosine, on Vital Behavioral Activities in Caenorhabditis elegans

Anthelmintic Effect of Leucaena leucocephala Extract and Its Active Compound, Mimosine, on Vital Behavioral Activities in Caenorhabditis elegans

Environmental Sustainability: Bioactivity of Leucaena leucocephala Leaves and Pesticide Residue Analysis in Tomato Fruits

A Review of the Potency of Plant Extracts and Compounds from Key Families as an Alternative to Synthetic Nematicides: History, Efficacy, and Current Developments

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