Characterisation of Brucella suis isolates from Southeast Europe by multi-locus variable-number tandem repeat analysis

Duvnjak, Sanja; Račić, Ivana; Špičić, Silvio; Zdelar-Tuk, Maja; Reil, Irena; Cvetnić, Željko

doi:10.1016/j.vetmic.2015.08.013

Cited by 18 publications

(14 citation statements)

References 19 publications

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“…We assume that infection occurred through direct or indirect contact with wild boars. We associate this supposition with the results of investigations of other authors who point out that wild boars present the most frequent source of infection of outdoor pigs (15,16,29). The genetic similarity of strains originating from domestic and wild pigs from Hungary and strains from Germany and Croatia point to the fact that state borders are not an obstacle to the spread of pathogens (30).…”

Section: Resultssupporting

confidence: 57%

First report of Brucella suis biovar 2 in outdoor reared pigs(Sus scrofa domesticus) in Serbia

Žutić¹,

Cvetojevic²,

Veljović³

et al. 2017

Turk J Vet Anim Sci

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The objective of this work was to present the first case of Brucella suis biovar 2 isolation in outdoor reared pigs in Serbia. B. suis biovar 2 has not previously been detected in Serbia, from either wild boar or outdoor reared pigs. In our case, brucellosis was discovered in the region of Srem, which geographically constitutes a part of northwestern Serbia. Outdoor reared pigs in the Srem region are part of an extensive breeding system in the field and woods. In the course of a random visit to a herd in the above-mentioned area, the animals' owner discovered two aborted fetuses, thereafter presented for laboratory examinations. B. suis biovar 2 was isolated from both fetuses. Isolated strains were identified using both classical and molecular techniques, including genomic sequencing. Based on epizootiological data, we were unable to establish the source of infection.

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Section: Resultssupporting

confidence: 57%

First report of Brucella suis biovar 2 in outdoor reared pigs(Sus scrofa domesticus) in Serbia

Žutić¹,

Cvetojevic²,

Veljović³

et al. 2017

Turk J Vet Anim Sci

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“…The presence of free-ranging pigs in some areas in the region may contribute to the possible spread of ASF in the ROC. This has been demonstrated in the past for other diseases, such as Aujeszky's disease or brucellosis in the ROC (Duvnjak et al, 2015;Milicevic et al, 2016;Zutic et al, 2017). Free-ranging pig farming is a traditional husbandry practice in most countries in the ROC.…”

Section: 1mentioning

confidence: 95%

“…In addition, it has been shown that infectious diseases are 'shared' between domestic pigs and wild boar. Studies on Brucella suis strains have provided evidence that wild boar might be a source of infections for domestic pigs (Duvnjak et al, 2015;Zutic et al, 2017). Other B. suis surveys suggested introduction of wild boar from central-Eastern Europe to Italy for hunting purposes (Di Sabatino et al 2017).…”

Section: 1mentioning

confidence: 99%

Risk assessment of African swine fever in the south‐eastern countries of Europe

Nielsen¹,

Álvarez²,

Bicout³

et al. 2019

EFS2

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The European Commission requested EFSA to estimate the risk of spread of African swine fever (ASF) and to identify potential risk factors (indicators) for the spread of ASF, given introduction in the southeastern countries of Europe (region of concern, ROC), . Three EU Member States (MS) -Croatia, Greece and Sloveniawere included in the ROC due to their geographical location and ASF-free status. Based on collected information on potential risk factors (indicators) for each country and the relevant EU regulations in force, the estimated probability of spread of ASF within the ROC within one year after introduction into the ROC was assessed to be very high (from 66% to 100%). This estimate was determined after considering the high number of indicators present in most of the countries in the ROC and the known effect that these indicators can have on ASF spread, especially those related to the structure of the domestic pig sector, the presence of wild boar and social factors.

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“…Our newly developed MLVA-13Bc method was specifically designed to discriminate strains from within the species of B . canis , and had higher discriminatory power than previous assays 25, 31, 32 . Although our data is not associated with pathogenesis as such information is not available, the new MLVA-13Bc method genotyped each of the 229 isolates in our collection, indicating that it is a useful molecular epidemiological tool to trace the source of strain introduction in an event of a B .…”

Section: Discussionmentioning

confidence: 61%

Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay

Yang

Wang

Poulsen

et al. 2017

Sci Rep

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Differentiation of Brucella canis from other Brucella species are mainly performed through PCR-based methods and multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) procedures. Both PCR-based and MLVA methods are limited in discriminating B. canis strains. A new MLVA-13Bc method for B. canis genotyping was established by combining eight newly-developed VNTRs with five published ones. During 2010 and 2016, 377 B. canis PCR-positives were identified from 6,844 canine blood samples from 22 U.S. states, resulting in 229 B. canis isolates. The MLVA-13Bc method was able to differentiate each of these 229 isolates. The Hunter-Gaston Discriminatory Index of the individual VNTR loci ranged from 0.516 to 0.934 and the combined discriminatory index reached 1.000. Three major clusters (A, B and C) and 10 genotype groups were identified from the 229 B. canis isolates. Cluster A mainly contains genotype groups 1 and 2, and a few group 3 isolates; nearly all Cluster B isolates were from group 6; other genotype groups were classified into Cluster C. Our newly developed MLVA-13Bc assay is a highly discriminatory assay for B. canis genotyping, and can serve as a useful molecular epidemiological tool, especially for tracing the source of contamination in an event of a B. canis outbreak.

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Characterisation of Brucella suis isolates from Southeast Europe by multi-locus variable-number tandem repeat analysis

Cited by 18 publications

References 19 publications

First report of Brucella suis biovar 2 in outdoor reared pigs(Sus scrofa domesticus) in Serbia

First report of Brucella suis biovar 2 in outdoor reared pigs(Sus scrofa domesticus) in Serbia

Risk assessment of African swine fever in the south‐eastern countries of Europe

Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay

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