Characterisation of circadian rhythms of various duckweeds

Muranaka, Tomoaki; Okada, Masaaki; Yomo, J.; Kubota, Saya; Oyama, Tokitaka

doi:10.1111/plb.12202

Cited by 37 publications

(65 citation statements)

References 40 publications

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“…Next, we generated knockout lines of Mp PRR , Mp TOC1 and Mp RVE in F1 sporelings derived from crosses between Tak‐1 and Tak‐2 (Figs S8–S10), and utilized a luciferase reporter controlled by a CaMV35S promoter (35S pro :LUC ) to assay bioluminescence in transgenic lines. As previously observed in the angiosperm Lemna gibba (Muranaka et al ., ), 35S pro :LUC lines showed diel oscillation under ND and persistent rhythm in LL for several days (Fig. ).…”

Section: Resultsmentioning

confidence: 99%

Early evolution of the land plant circadian clock

et al. 2017

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Summary While angiosperm clocks can be described as an intricate network of interlocked transcriptional feedback loops, clocks of green algae have been modelled as a loop of only two genes. To investigate the transition from a simple clock in algae to a complex one in angiosperms, we performed an inventory of circadian clock genes in bryophytes and charophytes. Additionally, we performed functional characterization of putative core clock genes in the liverwort Marchantia polymorpha and the hornwort Anthoceros agrestis.Phylogenetic construction was combined with studies of spatiotemporal expression patterns and analysis of M. polymorpha clock gene mutants.Homologues to core clock genes identified in Arabidopsis were found not only in bryophytes but also in charophytes, albeit in fewer copies. Circadian rhythms were detected for most identified genes in M. polymorpha and A. agrestis, and mutant analysis supports a role for putative clock genes in M. polymorpha.Our data are in line with a recent hypothesis that adaptation to terrestrial life occurred earlier than previously expected in the evolutionary history of charophyte algae. Both gene duplication and acquisition of new genes was important in the evolution of the plant circadian clock, but gene loss has also contributed to shaping the clock of bryophytes.

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Section: Resultsmentioning

confidence: 99%

Early evolution of the land plant circadian clock

et al. 2017

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“…To investigate the cell-to-cell movement of MP YoMV -GFP or GFP, we performed particle bombardment essentially according to the protocol described in a previous study (Muranaka et al, 2014). True leaves of A. thaliana were loaded onto a 0.4% agarose gel and bombarded using 900 psi rupture discs.…”

Section: Cell-to-cell Movement Assay By Particle Bombardmentmentioning

confidence: 99%

Structural and functional relationships between plasmodesmata and plant endoplasmic reticulum–plasma membrane contact sites consisting of three synaptotagmins

et al. 2020

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Summary Synaptotagmin 1 (SYT1) has been recognised as a tethering factor of plant endoplasmic reticulum (ER)–plasma membrane (PM) contact sites (EPCSs) and partially localises to around plasmodesmata (PD). However, other components of EPCSs associated with SYT1 and functional links between the EPCSs and PD have not been identified. We explored interactors of SYT1 by immunoprecipitation and mass analysis. The dynamics, morphology and spatial arrangement of the ER in Arabidopsis mutants lacking the EPCS components were investigated using confocal microscopy and electron microscopy. PD permeability of EPCS mutants was assessed using a virus movement protein and free green fluorescent protein (GFP) as indicators. We identified two additional components of the EPCSs, SYT5 and SYT7, that interact with SYT1. The mutants of the three SYTs were defective in the anchoring of the ER to the PM. The ER near the PD entrance appeared to be weakly squeezed in the triple mutant compared with the wild‐type. The triple mutant suppressed cell‐to‐cell movement of the virus movement protein, but not GFP diffusion. We revealed major additional components of EPCS associated with SYT1 and suggested that the EPCSs arranged around the PD squeeze the ER to regulate active transport via PD.

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“…For many years a group from Kyoto University has investigated circadian clocks in duckweeds (Miwa et al 2006). In their newest paper this group reports that circadian rhythms show diversity in period length and sustainability in a broad range of duckweed species, suggesting that circadian clock mechanisms are somewhat diversified among duckweeds (Muranaka et al 2015).…”

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confidence: 98%