Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy

Sokolova, Viktoriya; Ludwig, Anna‐Kristin; Hornung, Sandra; Rotan, Olga; Horn, Peter A.; Epple, Matthias; Giebel, Bernd

doi:10.1016/j.colsurfb.2011.05.013

Cited by 713 publications

(607 citation statements)

References 17 publications

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“…To investigate this, we initially qualified the nanoparticle tracking analysis (NTA) as EV-quantification method and optimized a polyethylene glycol (PEG)-based large scale EV-preparation method (74,75). Testing PEG purified MSC-EVs in a mixed lymphocyte reaction assay we observed that in the presence of MSC-EVs less immune cells secreted proinflammatory cytokines than in their absence.…”

Section: Gvhdmentioning

confidence: 99%

Clinical potential of mesenchymal stem/stromal cell-derived extracellular vesicles

Giebel

Kordelas²,

Börger

2017

Stem Cell Investig.

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131

115

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Within the last two decades mesenchymal stem/stromal cells (MSCs) emerged after hematopoietic stem cells as the second most investigated and applied somatic stem cell entity so far. MSCs mediate immunosuppressive as well as pro-regenerative activities. Against the initial assumption, MSCs may not primarily exert their therapeutic functions in a cellular but rather in a paracrine manner. Here, extracellular vesicles (EVs), such as exosomes and microvesicles, have been identified as major mediators of these paracrine effects. Meanwhile, MSC-EVs have been applied to an increasing amount of different animal models and were tested in a patient suffering from steroid-refractory acute graft-versus-host disease (acute GvHD) as well as in a patient cohort with chronic kidney disease. So far, the MSC-EV administration appears to be safe in humans and all tested animal models. Improvements were reported in all settings. Thus, MSC-EVs appear as promising novel therapeutic agents which might help to improve disease associated symptoms in millions of patients. Here, we review some of the milestones in the field, briefly discuss challenges and highlight clinical aspects of acute GvHD and its treatment with MSCs and MSC-EVs.

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Section: Gvhdmentioning

confidence: 99%

Clinical potential of mesenchymal stem/stromal cell-derived extracellular vesicles

Giebel

Kordelas²,

Börger

2017

Stem Cell Investig.

Self Cite

131

115

View full text Add to dashboard Cite

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“…Alix, TSG101) and in tetraspanins such as CD9, CD63, and CD81. As exosomes are generally smaller than the resolution limit of conventional optical techniques (light microscopy, flow cytometry scatter) (Van Der Pol et al 2010), highly dedicated technology is required for their detection, including electron microscopy, nanoparticle tracking analysis (NTA) (Sokolova et al 2011), tunable resistive pulse sensing (TRPS) (Maas, De Vrij, and Broekman 2014), and fluorescence-triggered high-resolution flow cytometry (Nolte- 'T Hoen et al 2012). Alternatively, exosomes may be characterized by bulk analysis techniques, such as Western blotting or bead-based flow cytome- try, a technique in which EV are linked to large beads and subsequently stained for exosome marker proteins (Suarez et al 2017;Volgers et al 2017).…”

Section: Exosomesmentioning

confidence: 99%

Extracellular vesicles released in response to respiratory exposures: implications for chronic disease

Benedikter

Wouters

Savelkoul

et al. 2018

Journal of Toxicology and Environmental Health, Part B

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Extracellular vesicles (EV) are secreted signaling entities that enhance various pathological processes when released in response to cellular stresses. Respiratory exposures such as cigarette smoke and air pollution exert cellular stresses and are associated with an increased risk of several chronic diseases. The aim of this review was to examine the evidence that modifications in EV contribute to respiratory exposure-associated diseases. Publications were searched using PubMed and Google Scholar with the search terms (cigarette smoke OR tobacco smoke OR air pollution OR particulate matter) AND (extracellular vesicles OR exosomes OR microvesicles OR microparticles OR ectosomes). All original research articles were included and reviewed. Fifty articles were identified, most of which investigated the effect of respiratory exposures on EV release in vitro (25) and/or on circulating EV in human plasma (24). The majority of studies based their main observations on the relatively insensitive scatter-based flow cytometry of EV (29). EV induced by respiratory exposures were found to modulate inflammation (19), thrombosis (13), endothelial dysfunction (11), tissue remodeling (6), and angiogenesis (3). By influencing these processes, EV may play a key role in the development of cardiovascular diseases and chronic obstructive pulmonary disease and possibly lung cancer and allergic asthma. The current findings warrant additional research with improved methodologies to evaluate the contribution of respiratory exposure-induced EV to disease etiology, as well as their potential as biomarkers of exposure or risk and as novel targets for preventive or therapeutic strategies.

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“…62 After separation, exosome identities are determined by at least two of these methods include atomic force microscopy (AFM), scanning electron microscopy (SEM), dynamic light scattering (DLS), flow cytometry (FCM), western blotting, nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) or ELISA. [63][64][65] After the isolation and characterization, exosomes for in vivo or in vitro applications must be frozen because they are unstable at room temperature and 37 °C. Exosomes can be stored for 6 months at -20 °C without cryopreservative agents.…”

Section: Isolation and Storage Of Exosomesmentioning

confidence: 99%

Mesenchymal Stem Cell-Derived Exosomes: New Opportunity in Cell-Free Therapy

2016

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Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy

Cited by 713 publications

References 17 publications

Clinical potential of mesenchymal stem/stromal cell-derived extracellular vesicles

Clinical potential of mesenchymal stem/stromal cell-derived extracellular vesicles

Extracellular vesicles released in response to respiratory exposures: implications for chronic disease

Mesenchymal Stem Cell-Derived Exosomes: New Opportunity in Cell-Free Therapy

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