Characterisation of Fasciola species from Mainland China by ITS-2 ribosomal DNA sequence

Huang, Weiyi; He, Bin; Wang, C.R; Zhu, Xing‐Quan

doi:10.1016/j.vetpar.2003.12.006

Cited by 139 publications

(121 citation statements)

References 13 publications

Supporting

Mentioning

112

Contrasting

Unclassified

Order By: Relevance

“…This intermediate genotype has been also found in Korean Fasciola flukes using D2 sequences of 28S rDNA [2], in Chinese flukes using ITS2 [5] and in Japanese specimens using ITS1 and ITS2 [7]. Furthermore, two Korean flukes that were Kor1 in ITS1and Kor2 in NDI were also detected in this study.…”

Section: Discussionsupporting

confidence: 83%

Molecular Characterization of Parthenogenic Fasciola sp. in Korea on the Basis of DNA Sequences of Ribosomal ITS1 and Mitochondrial NDI Gene

et al. 2005

View full text Add to dashboard Cite

ABSTRACT. Nucleotide sequences of ribosomal internal transcribed spacer (ITS1) and mitochondrial NADH dehydrogenase I (NDI) gene were analyzed to genetically characterize aspermic Fasciola forms in Korea. From the difference in ITS1 sequences, Korean flukes were divided into 3 haplotypes represented by Kor1, Kor2 and Kor1/2, which had nucleotides identical to F. hepatica, F. gigantica and those overlapped between the two species, respectively. NDI sequences also showed that Korean flukes could be classified into 3 distinct haplotypes (Kor1: F. hepatica-type, . The sequences of Kor1 and Kor2a were 100% identical to those of the haplotypes Fsp1and Fsp2, respectively, which are major Fasciola forms in Japan. These findings strongly suggest that aspermic Fasciola forms in Korea and Japan originated from same ancestors and have recently spread throughout both countries. KEY WORDS: Fasciola sp., haplotype, ITS1, Korea, NDI.

show abstract

Section: Discussionsupporting

confidence: 83%

Molecular Characterization of Parthenogenic Fasciola sp. in Korea on the Basis of DNA Sequences of Ribosomal ITS1 and Mitochondrial NDI Gene

et al. 2005

View full text Add to dashboard Cite

show abstract

“…To update the information, the present review is being presented here. As observed, ITS-1 and ITS-2 of rDNA provided trustworthy markers for molecular systematics along with mitochondrial DNA (Huang et al, 2004;Razo-Mendivil et al, 2004;Miura et al, 2005;Maurelli et al, 2007;Al-Kandari et al, 2011;Caffara et al, 2011;Huang et al, 2012;Bott et al, 2013;Presswell et al, 2014). Only 47 studies mentioned the intra-specifi c (0.05 -10.9 %) or inter-specifi c variations (0.01 -16 %) and inter-generic variations of 2.67 -21.0 %.…”

Section: Resultsmentioning

confidence: 79%

“…Huang et al (2004) sequenced ITS-2 rDNA of Fasciola species from China and found no variation in length or composition from France, Sichuan and Guangxi region, while the sequence difference of 1.7 % (6/362) was observed. Fasciola from Sichuan as F. hepatica, Guangxi as F. gigantica and Heilongjiang reported as an intermediate genotype, as the ITS-2 sequences were unique.…”

Section: Fasciolidae (Railliet 1895)mentioning

confidence: 97%

A review on the molecular characterization of digenean parasites using molecular markers with special reference to ITS region

et al. 2015

View full text Add to dashboard Cite

SummaryThe rDNA region of eukaryotes has the immense potential to resolve the evolutionary and phylogeny problems using molecular markers. As evident from the present review, ITS region data is considered for interpretation of inter and intra-specifi c variations of 136 studies of 33 families including 78 genus and 114 species affecting individuals worldwide. Along with ITS-1 and ITS-2 region in 29 studies 18S region, in 38 studies 28S region and in 43 studies mitochondrial genes (COI and NDI) were also analyzed. Three new genera (Allobilharzia gen. nov., Caulanus gen. nov., and Latuterus gen. nov.) and 49 new species were discovered. Only 47 studies expressed variations at intra-specifi c and inter-specifi c level in complete ITS region, ITS-1 and ITS-2 rDNA sequences due to differences in nucleotide positions. According to the fi ndings ITS region is more reliable and precise marker for demarcation and identifi cation of species in combination of other DNA markers. Major studies were involved around the parasites of families Fasciolidae, Schistosomatidae, Opisthorchidae, Paragonimidae and Paramphistomidae, Clinostomidae, Diplostomidae, Haploporidae, among others infecting humans, farm animals, birds, fi shes, reptiles and amphibians on the clinical basis. In future, molecular and bioinformatics aspects based on genetic variations will lead to explore the untouched areas of trematodes.

show abstract

“…No intraspecific variations in the maps of F. hepatica or of F. gigantica were detected, but length heterogeneity was noted in the intergenic spacer, even within individual worms (Blair & McManus, 1989). The ITS-2 of the rDNA has been used several times for liver flukes differentiation (Itagaki & Tsutsumi, 1998;Andrews, 1999;Huang et al, 2004). One nucleotide difference in a 263 bp long ITS-2 fragment between the F. hepatica population from Mexico and those from Australia, Hungary and New Zealand, and no difference in the 213 bp long ITS-2 fragment compared between F. gigantica from Indonesia and Malaysia were found (Andrews, 1999).…”

Section: Resultsmentioning

confidence: 99%

Molecular differentiation of Turkey cattle isolates of Fasciola hepatica and Fasciola gigantica

Şimşek

Ütük²,

Balkaya

2011

Helminthologia

View full text Add to dashboard Cite

The most common and widespread liver flukes of the genus Fasciola are Fasciola hepatica and F. gigantica. Adults of both species occur in many domestic ruminants and in humans and can cause serious disease. The differential diagnosis of these flukes infection is very important because of their different transmission and epidemiological characteristics. A simple and rapid PCR-restriction fragment length polymorphism (RFLP) assay, using the common restriction enzymes AluI and RsaI, is described to distinguish between both fasciolid species. After the digestion of the mitochondrial cytochrome c oxidase 1 (CO1) PCR product with the restriction enzyme AluI, the RFLP profile obtained from F. hepatica revealed two fragments, whereas F. gigantica was not cut. The RsaI digestion generated two fragments from F. gigantica, whereas it did not cut the PCR product from F. hepatica. Results were confirmed with CO1 sequence analysis of both F. hepatica and F. gigantica. The present study suggests that the PCRRFLP method described here can be used for the proper identification of Fasciola species.

show abstract

Characterisation of Fasciola species from Mainland China by ITS-2 ribosomal DNA sequence

Cited by 139 publications

References 13 publications

Molecular Characterization of Parthenogenic Fasciola sp. in Korea on the Basis of DNA Sequences of Ribosomal ITS1 and Mitochondrial NDI Gene

Molecular Characterization of Parthenogenic Fasciola sp. in Korea on the Basis of DNA Sequences of Ribosomal ITS1 and Mitochondrial NDI Gene

A review on the molecular characterization of digenean parasites using molecular markers with special reference to ITS region

Molecular differentiation of Turkey cattle isolates of Fasciola hepatica and Fasciola gigantica

Contact Info

Product

Resources

About