Characterisation of insoluble α-1,3-/α-1,6 mixed linkage glucan produced in addition to soluble α-1,6-linked dextran by glucansucrase (DEX-N) from Leuconostoc citreum ABK-1

Wangpaiboon, Karan; Waiyaseesang, Nisachon; Panpetch, Pawinee; Charoenwongpaiboon, Thanapon; Nepogodiev, Sergey A.; Ekgasit, Sanong; Field, Robert A.; Pichayangkura, Rath

doi:10.1016/j.ijbiomac.2020.02.247

Cited by 28 publications

(13 citation statements)

References 55 publications

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“…citreum ABK-1 that is responsible for the synthesis of two types of glucans from sucrose. The first one is a soluble dextran composed of 90% α-1,6 linkages while the second one is insoluble and harbors a block-wise pattern of α-1,3 and α-1,6 osidic bonds [ 75 ].…”

Section: Inventory Of Characterized Gss and Brssmentioning

confidence: 99%

Bacterial α-Glucan and Branching Sucrases from GH70 Family: Discovery, Structure–Function Relationship Studies and Engineering

et al. 2021

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Glucansucrases and branching sucrases are classified in the family 70 of glycoside hydrolases. They are produced by lactic acid bacteria occupying very diverse ecological niches (soil, buccal cavity, sourdough, intestine, dairy products, etc.). Usually secreted by their producer organisms, they are involved in the synthesis of α-glucans from sucrose substrate. They contribute to cell protection while promoting adhesion and colonization of different biotopes. Dextran, an α-1,6 linked linear α-glucan, was the first microbial polysaccharide commercialized for medical applications. Advances in the discovery and characterization of these enzymes have remarkably enriched the available diversity with new catalysts. Research into their molecular mechanisms has highlighted important features governing their peculiarities thus opening up many opportunities for engineering these catalysts to provide new routes for the transformation of sucrose into value-added molecules. This article reviews these different aspects with the ambition to show how they constitute the basis for promising future developments.

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Section: Inventory Of Characterized Gss and Brssmentioning

confidence: 99%

Bacterial α-Glucan and Branching Sucrases from GH70 Family: Discovery, Structure–Function Relationship Studies and Engineering

et al. 2021

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“…These spectra showed good agreement with literature data for long α-1,6-glucans. 32,36 The respective positions of anomeric signals at 4.89 and 97.7 ppm for S-glucan of Gtf1 or 4.88 and 97.7 ppm for S-glucan of LEUM1747 in the 1 H and 13 C NMR spectra, respectively, are characteristic of α-1,6-glucosidic linkages. The 1 H NMR and 13 C NMR spectra of S-glucans of Gtf2 and LEUM1752 indicated the presence of both α-1,6and α-1,3-glucosidic chains.…”

Section: Production and Characterization Of Glucansmentioning

confidence: 99%

“…GUT MICROBES e1949097-5 mainly harboring α-1,3-linked glucose are generally water-insoluble, whereas glucans consisting of a high level of α-1,6-linked glucose are soluble in water. 36,38,39 A high ratio of 1,3-linked glucose might cause precipitation of P-glucans. The 1 H NMR and 13 C NMR spectra of S-glucans of Gtf1 and LEUM1747 showed a single set of signals (Figure 6).…”

Section: Production and Characterization Of Glucansmentioning

confidence: 99%

“…In the 1 H NMR spectra of S-glucans of Gtf2 and LEUM1752, although the anomeric proton signal of the α-1,6-glucosyl unit was hidden under the HOD signal, the chemical shift of 5.02 ppm can be assigned to anomeric protons of α-1,3-glucosyl unit. 36 In the 13 C NMR spectra of S-glucans of Gtf2 and LEUM1752, the positions of anomeric signals at 101.8 and 98.7 ppm are characteristic of α-1,3-and α-1,6-glucosidic linkages, respectively. 36…”

Section: Production and Characterization Of Glucansmentioning

confidence: 99%

“…36 In the 13 C NMR spectra of S-glucans of Gtf2 and LEUM1752, the positions of anomeric signals at 101.8 and 98.7 ppm are characteristic of α-1,3-and α-1,6-glucosidic linkages, respectively. 36…”

Section: Production and Characterization Of Glucansmentioning

confidence: 99%

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Enzymatically synthesized exopolysaccharide of a probiotic strain Leuconostoc mesenteroides NTM048 shows adjuvant activity to promote IgA antibody responses

et al. 2021

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Leuconostoc mesenteroides strain NTM048 produces an exopolysaccharide (EPS; glucose polymers 94% and fructose polymers 6%) with adjuvanticity for mucosal vaccination. Strain NTM048 includes three putative EPS-synthesizing genes, gtf1 and gtf2 for synthesizing glucose polymers, and lvnS for synthesizing fructose polymer. To elucidate the key polymer structure for adjuvanticity, two genes, gtf1 and gtf2, which were annotated as glycoside hydrolase family 70 enzyme genes, were expressed in Escherichia coli. Glycosyl-linkage composition analysis and NMR analysis showed that the recombinant enzyme Gtf1 produced a soluble form of α-1,6-glucan, whereas the recombinant enzyme Gtf2 produced glucans with approximately equal percentages of α-1,6-and α-1,3-glucose residues both in the supernatant (S-glucan) and as a precipitate (P-glucan). Comparison of polysaccharides synthesized by Gtf1, Gtf2, and LvnS revealed that Gtf2-S-glucan, which was produced in the supernatant by Gtf2 and formed particles of 7.8 µm, possessed 1.8-fold higher ability to stimulate IgA production from murine Peyer's patch cells than native NTM048 EPS. Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigenspecific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2019–2020

Harvey

2023

Mass Spectrometry Reviews

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This review is the tenth update of the original article published in 1999 on the application of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2020. Also included are papers that describe methods appropriate to analysis by MALDI, such as sample preparation techniques, even though the ionization method is not MALDI. The review is basically divided into three sections: (1) general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, quantification and the use of arrays. (2) Applications to various structural types such as oligo‐ and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals, and (3) other areas such as medicine, industrial processes and glycan synthesis where MALDI is extensively used. Much of the material relating to applications is presented in tabular form. The reported work shows increasing use of incorporation of new techniques such as ion mobility and the enormous impact that MALDI imaging is having. MALDI, although invented nearly 40 years ago is still an ideal technique for carbohydrate analysis and advancements in the technique and range of applications show little sign of diminishing.

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Characterisation of insoluble α-1,3-/α-1,6 mixed linkage glucan produced in addition to soluble α-1,6-linked dextran by glucansucrase (DEX-N) from Leuconostoc citreum ABK-1

Cited by 28 publications

References 55 publications

Bacterial α-Glucan and Branching Sucrases from GH70 Family: Discovery, Structure–Function Relationship Studies and Engineering

Bacterial α-Glucan and Branching Sucrases from GH70 Family: Discovery, Structure–Function Relationship Studies and Engineering

Enzymatically synthesized exopolysaccharide of a probiotic strain Leuconostoc mesenteroides NTM048 shows adjuvant activity to promote IgA antibody responses

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2019–2020

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