Characterisation of micromonosporae from aquatic environments using molecular taxonomic methods

Abstract

Large numbers of strains assigned to the genus Micromonospora on the basis of typical colonial and pigmentation features were isolated from diverse aquatic sediments using a standard selective isolation procedure. Two hundred and six isolates and eight representatives of the genus Micromonospora were assigned to 24 multimembered groups based on a numerical analysis of banding patterns generated using BOX and ERIC primers. Representatives of multimembered groups encompassing isolated micromonosporae were the subject of 16S rRNA gene sequencing analyses. Good congruence was found between the molecular fingerprinting and 16S rRNA sequence data indicating that the groups based upon the former are taxonomically meaningful. Nearly all of the isolates that were chosen for the 16S rRNA gene sequencing analyses showed that the ecosystems studied are a rich source of novel micromonosporae. These findings have implications for high throughput screening for novel micromonosporae as BOX and ERIC fingerprinting, which is rapid and reproducible, can be applied as a robust dereplication procedure to indicate which environmental isolates have been cultured previously.

The genus Micromonospora is widespread in legume root nodules: the example of Lupinus angustifolius

“…There is indeed strong evidence suggesting that Micromonospora type strains, genomically defined by lower than about 70% DNA-DNA reassociation values, are delineated by threshold values of almost 99.3% 16S rRNA gene sequence similarities (Kasai et al, 2000;Thawai et al, 2007;Trujillo et al, 2006Trujillo et al, , 2007Jongrungruangchok et al, 2008;Maldonado et al, 2008). In this study, many of the strains sequenced were found in this range.…”

“…BOX-PCR fingerprinting has been shown to be a useful tool to discriminate highly related strains and has been applied to study the genetic diversity of different bacterial taxa including Micromonospora (Lanoot et al, 2004;Maldonado et al, 2008;Torres et al, 2008). To get more insight into the genetic diversity of the Micromonospora strains isolated, they were analysed by BOX-PCR fingerprinting.…”

Diacidene, a Polyene Dicarboxylic Acid from a Micromonospora Isolate from the German Wadden Sea

“…Marine sediments have proven a profi table habitat for the isolation of high numbers of Micromonosporaceae (Bredholt et al, 2008;Maldonado et al, 2008;Prieto-Davó et al, 2008). Our isolate, Micromonospora strain DB620, originated from the German Wadden Sea, a unique ecosystem characterized by large areas of tidal fl ats.…”

Diacidene, a Polyene Dicarboxylic Acid from a Micromonospora Isolate from the German Wadden Sea

“…Marine sediments have proven a profi table habitat for the isolation of high numbers of Micromonosporaceae (Bredholt et al, 2008;Maldonado et al, 2008;Prieto-Davó et al, 2008). Our isolate, Micromonospora strain DB620, originated from the German Wadden Sea, a unique ecosystem characterized by large areas of tidal fl ats.…”