Characterisation of multidrug-resistant Salmonella Typhimurium 4,[5],12:i:- DT193 strains carrying a novel genomic island adjacent to the thrW tRNA locus

Genotyping of selected bacterial enteropathogens in Norway

“…The monophasic isolates were fully typeable and grouped together with the S. typhimurium isolates, indicating that these isolates most likely originated from S. typhimurium. This view is supported by several studies (Ido et al, 2010;Laorden et al, 2010;Switt et al, 2009;Trupschuch et al, 2010). Our MLVA method displayed 170 (34.8%) of 489 isolates where all five loci were present and typeable.…”

Infection of chickens with antimicrobial-resistantSalmonella entericaTyphimurium DT193 and monophasicSalmonellaTyphimurium-like variants: an emerging risk to the poultry industry?

“…Among these antibiotic-resistant Salmonella strains perhaps the greatest threat to public health are atypical monophasic variants of S. Typhimurium (EFSA, 2010). These are frequently associated with invasive human gastroenteritis (Trupschuch et al, 2010;Wasyl & Hoszowski, 2012).…”

“…Monophasic members of Salmonella predominantly belong to two main phage types, DT193 and DT104, and pigs are considered the predominant source Huehn et al, 2010;Wasyl & Hoszowski, 2012). In Germany around 40% of human S. Typhimurium isolates submitted to the National Reference Centre are monophasic variants (Trupschuch et al, 2010). Molecular analysis has found that monophasic members of Salmonella isolated from pigs, pork and humans display high levels of genetic homology .…”

The zoonotic agent Salmonella

“…In Germany this serovar attracted attention in 2006 due to its rapid increase (Trupschuch et al 2010). As shown in Fig.…”

“…Homologous sequences for several parts of the island were found in E. coli and Shigella genomes indicating a stepwise acquisition of the element. Alignment analyses revealed mainly phage-related gene products (Trupschuch et al 2010). Although the relevance of the novel island for bacterial fitness or pathogenicity in the emerging monophasic variant of S. Typhimurium is not yet understood it seems to improve the resistance towards lytic phages.…”