Characterisation of polymorphic microsatellite markers in the widespread Australian seagrass, Posidonia australis Hook. f. (Posidoniaceae), with cross-amplification in the sympatric P. sinuosa

Abstract

We developed 10 polymorphic microsatellite markers in the Australian seagrass Posidonia australis Hook. f. Markers were screened for their ability to detect within-and among-population genetic structure and variation. The markers showed a range in levels of polymorphism from fixed differences between the two sampled seagrass meadows to high levels of heterozygosity. These markers will be used to estimate gene flow across the species range, characterise the mating system through paternity analysis and pollen dispersal, characterise the nature and extent of clonality, and determine the genetic differentiation of local seagrass meadows to provide information on where to source local genetic provenance material for seagrass restoration projects. Seven of the 10 loci also amplified in the sympatric P. sinuosa and will be useful in future studies in population genetics and hybridisation.

Genetic signatures of Bassian glacial refugia and contemporary connectivity in a marine foundation species

“…Nine polymorphic microsatellite loci ( Pa A1, Pa A105 , Pa A120 , Pa B6 Pa B8 , Pa B112 , Pa D12, Pa D113, Pa 118/9) were genotyped using methods described in Sinclair et al . (, ). We also included data for three meadows from southern New South Wales (Evans et al ., ).…”

“…Collection, handling and laboratory methods are described in Sinclair et al (2014). Nine polymorphic microsatellite loci (PaA1, PaA105, PaA120, PaB6 PaB8, PaB112, PaD12, PaD113, Pa118/9) were genotyped using methods described in Sinclair et al (2009Sinclair et al ( , 2014. We also included data for three meadows from southern New South Wales (Evans et al, 2014).…”

A genetic assessment of a successful seagrass meadow (Posidonia australis) restoration trial

“…DNA was extracted from shoot meristem and genotyped using seven polymorphic markers (Sinclair et al . ). Genetic (clonal) diversity within meadows was assessed using the software GENALEX v6.1 (Peakall & Smouse ).…”

Microsatellite markers from the Ion Torrent: a multi‐species contrast to 454 shotgun sequencing

“…We used the Sinclair et al . () extraction method for the two gymnosperm species and a modified version of the Carlson et al . () extraction method for the angiosperm.…”

“…We extracted DNA from leaf material from one individual of each of the two gymnosperm species, Macrozamia riedlei (Cycadaceae; MR) and Podocarpus drouynianus (Podocarpaceae; PD), and the angiosperm species, Ricinocarpos brevis (Euphorbiaceae; RB). We used the Sinclair et al (2009) extraction method for the two gymnosperm species and a modified version of the Carlson et al (1991) extraction method for the angiosperm. The modifications involved adding a potassium acetate step (0.7 M KAc in supernatant, freeze for 15 min, centrifuge at 13 000 g for 10 min and transfer supernatant) before the isopropanol step and adding a sodium chloride step (3 M NaCl in dissolved pellet solution, centrifuge at 16 600 g for 10 min and discard the supernatant) before ethanol precipitation.…”