Characterisation of the bacterial microbiota of a landfill-contaminated confined aquifer undergoing intrinsic remediation

Abiriga, Daniel; Jenkins, Andrew; Alfsnes, Kristian; Vestgarden, Live Semb; Klempe, Harald

doi:10.1016/j.scitotenv.2021.147349

Cited by 20 publications

(2 citation statements)

References 54 publications

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“…Among the identified bacteria were Aequorivita, Balneola, Cellvibrio, Clostridium Flavobacterium, Fluviicola, Stenotrophomonas, Parvibaculum, Perlucidibaca and Verrucomicrobium, previously described as characteristic of sites associated with MSW disposal [39,41,50,51].…”

Section: Biodiversity Of Air Leachate and Soilmentioning

confidence: 87%

Assessment of Physicochemical, Microbiological and Toxicological Hazards at an Illegal Landfill in Central Poland

Szulc

Okrasa

Nowak

et al. 2022

IJERPH

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This study aimed to assess the physicochemical, microbiological and toxicological hazards at an illegal landfill in central Poland. The research included the analysis of airborne dust (laser photometer), the number of microorganisms in the air, soil and leachate (culture method) and the microbial diversity in the landfill environment (high-throughput sequencing on the Illumina Miseq); the cytotoxicity (PrestoBlue) and genotoxicity (alkaline comet assay) of soil and leachate were tested. Moreover, an analysis of UHPLC-Q-ToF-UHRMS (ultra-high-performance liquid chromatography-quadrupole-time-of-flight ultrahigh-resolution mass spectrometry) was performed to determine the toxic compounds and microbial metabolites. The PM1 dust fraction constituted 99.89% and 99.99% of total dust and exceeded the threshold of 0.025 mg m−3 at the tested locations. In the air, the total number of bacteria was 9.33 × 101–1.11 × 103 CFU m−3, while fungi ranged from 1.17 × 102 to 4.73 × 102 CFU m−3. Psychrophilic bacteria were detected in the largest number in leachates (3.3 × 104 to 2.69 × 106 CFU mL−1) and in soil samples (8.53 × 105 to 1.28 × 106 CFU g−1). Bacteria belonging to Proteobacteria (42–64.7%), Bacteroidetes (4.2–23.7%), Actinobacteria (3.4–19.8%) and Firmicutes (0.7–6.3%) dominated. In the case of fungi, Basidiomycota (23.3–27.7%), Ascomycota (5.6–46.3%) and Mortierellomycota (3.1%) have the highest abundance. Bacteria (Bacillus, Clostridium, Cellulosimicrobium, Escherichia, Pseudomonas) and fungi (Microascus, Chrysosporium, Candida, Malassezia, Aspergillus, Alternaria, Fusarium, Stachybotrys, Cladosporium, Didymella) that are potentially hazardous to human health were detected in samples collected from the landfill. Tested leachates and soils were characterised by varied cyto/genotoxins. Common pesticides (carbamazepine, prometryn, terbutryn, permethrin, carbanilide, pyrethrin, carbaryl and prallethrin), quaternary ammonium compounds (benzalkonium chlorides), chemicals and/or polymer degradation products (melamine, triphenylphosphate, diphenylphtalate, insect repellent diethyltoluamide, and drugs (ketoprofen)) were found in soil and leachate samples. It has been proven that the tested landfill is the source of the emission of particulate matter; microorganisms (including potential pathogens) and cyto/genotoxic compounds.

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Section: Biodiversity Of Air Leachate and Soilmentioning

confidence: 87%

Assessment of Physicochemical, Microbiological and Toxicological Hazards at an Illegal Landfill in Central Poland

Szulc

Okrasa

Nowak

et al. 2022

IJERPH

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“…Open dumping exposes the waste to several environmental variables such as precipitation and moisture, which leads to the generation of complex landfill leachate (Abiriga et al 2021a). Landfill leachate is complex effluent, which is known to contain dissolved organic substances, inorganic substances (ammonia, calcium, magnesium, sodium, potassium, iron, sulphate, chloride etc.…”

Section: Introductionmentioning

confidence: 99%

How much spatial and temporal variation in groundwater microbiology can occur following open dumping of municipal solid waste?

Kumar

Verma

Iqbal

et al. 2022

Water Practice and Technology

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Effects of open dumping of municipal solid waste on local groundwater microbiology were assessed in Ranchi, India. Both the spatial and temporal variations of groundwater microbiology were studied. Groundwater was analyzed to determine heterotrophic plate count (HPC), total coliforms (TC) and faecal coliforms (FC). Highest HPC was 4.5 × 104 CFU/mL and the highest total coliform count was 3.7 × 104 CFU/mL. Faecal coliforms were detected in most of the groundwater samples. The highest faecal coliform count of 2.1 × 104 CFU/mL was from a hand pump located adjacent to the dumping site. Spatial variations revealed dispersion of bacterial contamination up to 5 km from the periphery of the municipal solid waste dump. The level of bacterial contamination decreased with an increase in distance from the municipal solid waste dump. The maximum bacterial count was detected in the monsoon whereas the lowest count was found in summer. Molecular characterization of morphologically similar faecal coliform colonies indicated the presence of Klebsiella pneumoniae (Klebsiella ssnkbit, KU647674) in one of the groundwater samples which showed maximum faecal coliform count. Results of this study indicated that groundwater in the area is highly contaminated with a bacterial load which can be a major health risk.

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Metabarcoding Approach in Identifying Potential Pollutant Degraders

Ottoni

Passarini

Bonugli-Santos

2022

Omics Insights in Environmental Bioremediation

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Characterisation of the bacterial microbiota of a landfill-contaminated confined aquifer undergoing intrinsic remediation

Cited by 20 publications

References 54 publications

Assessment of Physicochemical, Microbiological and Toxicological Hazards at an Illegal Landfill in Central Poland

Assessment of Physicochemical, Microbiological and Toxicological Hazards at an Illegal Landfill in Central Poland

How much spatial and temporal variation in groundwater microbiology can occur following open dumping of municipal solid waste?

Metabarcoding Approach in Identifying Potential Pollutant Degraders

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