Characterisation of the cell suspension harvested from the dermal epidermal junction using a ReCell® kit

Adipose-Derived Regenerative Cell Therapy for Burn Wound Healing: A Comparison of Two Delivery Methods

“…25,30 Using a matched pair analysis model, planimetry data showed that the percent of wound epithelialization and absolute epithelialized area were greater in wounds receiving topical spray and local injection of ADRCs compared to respective control. Although the inflammatory process is essential for wound healing, excessive inflammation can hamper the repair process.…”

Successful application of keratinocyte suspension using autologous fibrin spray

“…There is no wastage of the sprayed cells, unlike some of the digitalpressured aerosolised spray which are known to clog up around the nozzle when the cell suspension concentration is too thick. The cell viability was demonstrated to be unaffected with air pressure up to 10 psi [15], with numerous studies demonstrating the viability of the epidermal cells afteraerosolisation [11,15,16].…”

“…The viability of the cell suspension is viable up to four hours at room temperature and 24 h at 40 8C [11].…”

Randomized, Paired-Site Comparison of Autologous Engineered Skin Substitutes and Split-Thickness Skin Graft for Closure of Extensive, Full-Thickness Burns

“…Cultured epithelial autografts applied as partially-stratified, keratinocyte sheets have been studied extensively 7,8 , but are reported to blister, ulcerate, and remain mechanically fragile due to deficient formation of basement membrane 9 . Cultured keratinocytes have also been sprayed as cell suspensions 10 over partial-thickness burns 11 , or a dermal substitute 12,13 , but the time to healing may be lengthy due to the slow organization of the cultured cell suspensions into stratified, keratinized epidermis. Replacement of dermal tissue has also been shown to reduce long-term morbidity from scarring.…”