Characterisation of the intestinal microbial communities of rainbow trout (Oncorhynchus mykiss) fed with Hermetia illucens (black soldier fly) partially defatted larva meal as partial dietary protein source

Bruni, Leonardo; Pastorelli, Roberta; Viti, Carlo; Gasco, Laura; Parisi, Giuliana

doi:10.1016/j.aquaculture.2018.01.006

Cited by 161 publications

(160 citation statements)

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“…Diet is one of the key factors in shaping the fish intestinal microbiota. In agreement with previous findings in rainbow trout (29,58,59) and laying hens (60, 61), we found that the insect meal diet altered the distal intestinal microbiota assemblage resulting in higher microbial richness and diversity. Our findings, showing that the insect meal diet increased the relative abundance of Actinomyces, Bacillus, Brevibacterium, Corynebacterium 1 and Enterococcus, are in accord with recent studies in rainbow trout fed diets containing 30% black soldier fly larvae meal (29,59).…”

Section: Sample Origin Effectsupporting

confidence: 93%

Differential Response of Digesta- and Mucosa-Associated Intestinal Microbiota to Dietary Black Soldier Fly (Hermetia illucens) Larvae Meal in Seawater Phase Atlantic Salmon (Salmo salar)

Bruni

Jaramillo-Torres

et al. 2020

Preprint

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Intestinal digesta is commonly used for studying responses of microbiota to dietary shifts, yet evidence is accumulating that it represents an incomplete view of the intestinal microbiota. In a 16-week seawater feeding trial, Atlantic salmon (Salmo salar) were fed either a commercially-relevant reference diet or an insect meal diet containing 15% black soldier fly (Hermetia illucens) larvae meal. The digesta-and mucosa-associated distal intestinal microbiota were profiled by 16S rRNA gene sequencing. Regardless of diet, we observed substantial differences between digesta-and mucosa-associated intestinal microbiota. Microbial richness and diversity were much higher in the digesta than the mucosa. The insect meal diet altered the distal intestinal microbiota resulting in higher microbial richness and diversity. The diet effect, however, depended on the sample origin. Digesta-associated intestinal microbiota showed more pronounced changes than the mucosa-associated microbiota. Lastly, multivariate association analyses identified two mucosa-enriched taxa, Brevinema andersonii and unclassified Spirochaetaceae, associated with the expression of genes related to immune responses and barrier function in the distal intestine, respectively. Overall, our data clearly indicate that responses in digesta-and mucosa-associated microbiota to dietary inclusion of insect meal differ, with the latter being more resilient to dietary changes. Microbiota | Gut biogeography | Atlantic salmon | Diet | Black soldier fly Correspondence: yanxianl@nmbu.no Characterizing intestinal microbiota and its associationsLi & Bruni et al. | bioRχiv | May 24, 2020 | 1-8 with host responses is an essential step towards identifying key microbial clades promoting fish health and welfare. Ultimately, a milestone in the fish microbiota research would be knowing how to selectively manipulate the microbiota to improve the growth performance, disease resistance and health status of farmed fish. The main aims of the work presented herein were (i) to compare distal intestinal microbiota of Atlantic salmon fed a commercially relevant diet and an insect meal-based diet, (ii) to further explore the dissimilarity between digesta-and mucosa-associated microbiota and the differences in their response to dietary changes, and (iii) to identify associations between microbial clades and host responses. This work was part of a larger study consisting of a freshwater and seawater feeding trial that aimed to investigate the nutritional value and possible health effects for Atlantic salmon of a protein-rich insect meal produced from black soldier fly (Hermetia illucens) larvae. The present work focuses on the intestinal microbiota in seawater phase Atlantic salmon fed an insect meal diet containing 15% black soldier fly larvae meal for 16 weeks. Results on feed utilization, growth performance, fillet quality, intestinal histomorphology and gene expression have been reported elsewhere (30-32). ResultsHereafter, different sample groups are named based on the combination of die...

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Section: Sample Origin Effectsupporting

confidence: 93%

Differential Response of Digesta- and Mucosa-Associated Intestinal Microbiota to Dietary Black Soldier Fly (Hermetia illucens) Larvae Meal in Seawater Phase Atlantic Salmon (Salmo salar)

Bruni

Jaramillo-Torres

et al. 2020

Preprint

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“…Similarly, we found significant increases in Bacillaceae (the family including Bacillus) in our study in fish fed diets with black soldier fly larvae as well as defatted-larvae diets. In addition, previous studies have found that feeding with black soldier fly larvae or krill-derived chitin can significantly change the gut microbiota in rainbow trout (Bruni, et al, 2018) and Atlantic cod (Zhou, et al, 2013).…”

Section: Dietary Chitin and Chitinase Producing Bacteriamentioning

confidence: 95%

“…yeast and microalgae) have been shown to alter diversity and abundance of gut bacteria in salmonid fishes (Desai, et al, 2012;Huyben, et al, 2018;Huyben, et al, 2017;Ingerslev, et al, 2014;Lyons, et al, 2017;Michl, et al, 2017;Nyman, et al, 2017). Recently, a study using gel electrophoresis based sequencing method has found that feeding black soldier fly meal to rainbow trout increased diversity of gut microbiota (Bruni, et al, 2018). A few studies have suggested that chitin, a long-chain polymer of N-acetylglucosamine derived from exoskeleton of insects and crustacean shells, acts as a substrate for chitinase producing bacteria that are not commonly found in the fish gut (Askarian, et al, 2012;Bruni, et al, 2018;Ringø, et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, a study using gel electrophoresis based sequencing method has found that feeding black soldier fly meal to rainbow trout increased diversity of gut microbiota (Bruni, et al, 2018). A few studies have suggested that chitin, a long-chain polymer of N-acetylglucosamine derived from exoskeleton of insects and crustacean shells, acts as a substrate for chitinase producing bacteria that are not commonly found in the fish gut (Askarian, et al, 2012;Bruni, et al, 2018;Ringø, et al, 2012). Similarly, feeding krill-based chitin has been found to alter the gut microbiota of Atlantic salmon (Askarian, et al, 2012) and Atlantic cod (Zhou, et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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High-throughput sequencing of gut microbiota in rainbow trout (Oncorhynchus mykiss) fed larval and pre-pupae stages of black soldier fly (Hermetia illucens)

et al. 2019

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“…Insects have also been proposed as a potential source of bioactive compounds. In this respect, it has been speculate that insect's antioxidant peptides, chitin and antimicrobial peptides could stimulate the immune system (Su et al 2017;Henry et al 2018;Komi et al 2018;Kvalsvik Stenberg et al 2019), and modulate gut microbiome in animals (Bruni et al 2018;Antonopoulou et al 2019;Biasato et al 2019b;Rimoldi et al 2019), thus promoting health and welfare (J ozefiak and Engberg 2017; Gasco et al 2018b;Wu et al 2018).…”

Section: Strengthsmentioning

confidence: 99%

Insect and fish by-products as sustainable alternatives to conventional animal proteins in animal nutrition

Gasco

Acuti

Bani

et al. 2020

Italian Journal of Animal Science

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This paper reviews current knowledge on two feedstuffs, that is, insect meal and fish by-products, as alternatives to conventional animal protein sources. After an introductory part that highlights the need for sustainable development of animal production, the alternative protein sources are discussed. In particular, after providing some indications on their production and supply focussing on EU, a SWOT (Strengths, Weaknesses, Opportunities, and Threats) analysis was performed to identify the key factors that could help or impair the development of both protein sources production sectors. Finally, future perspectives are presented. The use of processed animal proteins derived from insects in farmed fish feeding is recognised by the EU legislation that authorises the use of proteins from seven insect species and the allowed substrates to rear insects. Insects have several advantages in nutritional value and the amino acid composition of their proteins generally meet animal requirements for good growth and health. The SWOT analysis indicated that insect meals can be considered as feed functional ingredients with beneficial properties that depend on the insect species, rearing system adopted, and the substrate used for their growth. Insects are expected to be increasingly used as a replacement for conventional animal-derived proteins, especially in aquafeeds. In the section regarding fishery and aquaculture by-products, the potential use of raw materials obtained during seafood processing is discussed. Peptides and amino acids recovered from as hydrolysed proteins can be used in animal feeds to partially substitute conventional protein feedstuffs thus providing nutrients, bioactive compounds and feed additives for animals. The SWOT analysis identified opportunities and weaknesses. Both the alternative protein sources are promising alternative feed ingredients for livestock production. HIGHLIGHTSThe sustainable development of animal production sector needs alternative protein sources for feeds formulation. Insects and fishery-and aquaculture by-products represent optimal alternative protein sources. A SWOT analysis has identified the key factors for the development of both protein sources production sectors. ARTICLE HISTORY

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Characterisation of the intestinal microbial communities of rainbow trout (Oncorhynchus mykiss) fed with Hermetia illucens (black soldier fly) partially defatted larva meal as partial dietary protein source

Cited by 161 publications

References 71 publications

Differential Response of Digesta- and Mucosa-Associated Intestinal Microbiota to Dietary Black Soldier Fly (Hermetia illucens) Larvae Meal in Seawater Phase Atlantic Salmon (Salmo salar)

Differential Response of Digesta- and Mucosa-Associated Intestinal Microbiota to Dietary Black Soldier Fly (Hermetia illucens) Larvae Meal in Seawater Phase Atlantic Salmon (Salmo salar)

High-throughput sequencing of gut microbiota in rainbow trout (Oncorhynchus mykiss) fed larval and pre-pupae stages of black soldier fly (Hermetia illucens)

Insect and fish by-products as sustainable alternatives to conventional animal proteins in animal nutrition

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