Characterisation of two gene subunits on the 1R chromosome of rye as orthologs of each of the Glu-1 genes of hexaploid wheat

Bustos, Alfredo de; Rubio, P.; Jouve, N.

doi:10.1007/s001220100669

Cited by 36 publications

(30 citation statements)

References 22 publications

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“…Sequence conservation is mostly located in the two HMW-glutenin regions, despite the fact that both genes are inactive. This may indicate that the inactivation of these genes occurred relatively recently, since disruption of the HMW-glutenin genes was not detected in the diploid rye (De Bustos et al, 2001), the D genome from A. tauschii , nor in the A m genome from Triticum monococcum (Y. Gu, S. Heath, and O. Anderson, unpublished data), a species closely related to the hexaploid A genome donor, T. urartu (Dvorak et al, 1992). Nevertheless, the possibility that the gene inactivation occurred in the diploid ancestral A genome cannot be ruled out.…”

Section: Comparing Paralogous and Orthologous Regions Among Wheat Genmentioning

confidence: 99%

Rapid Genome Evolution Revealed by Comparative Sequence Analysis of Orthologous Regions from Four Triticeae Genomes

Coleman‐Derr²,

Kong³

et al. 2004

Plant Physiology

124

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Bread wheat (Triticum aestivum) is an allohexaploid species, consisting of three subgenomes (A, B, and D). To study the molecular evolution of these closely related genomes, we compared the sequence of a 307-kb physical contig covering the high molecular weight (HMW)-glutenin locus from the A genome of durum wheat (Triticum turgidum, AABB) with the orthologous regions from the B genome of the same wheat and the D genome of the diploid wheat Aegilops tauschii Kong et al., 2004). Although gene colinearity appears to be retained, four out of six genes including the two paralogous HMWglutenin genes are disrupted in the orthologous region of the A genome. Mechanisms involved in gene disruption in the A genome include retroelement insertions, sequence deletions, and mutations causing in-frame stop codons in the coding sequences. Comparative sequence analysis also revealed that sequences in the colinear intergenic regions of these different genomes were generally not conserved. The rapid genome evolution in these regions is attributable mainly to the large number of retrotransposon insertions that occurred after the divergence of the three wheat genomes. Our comparative studies indicate that the B genome diverged prior to the separation of the A and D genomes. Furthermore, sequence comparison of two distinct types of allelic variations at the HMW-glutenin loci in the A genomes of different hexaploid wheat cultivars with the A genome locus of durum wheat indicates that hexaploid wheat may have more than one tetraploid ancestor.

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Section: Comparing Paralogous and Orthologous Regions Among Wheat Genmentioning

confidence: 99%

Rapid Genome Evolution Revealed by Comparative Sequence Analysis of Orthologous Regions from Four Triticeae Genomes

Coleman‐Derr²,

Kong³

et al. 2004

Plant Physiology

124

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“…The duplication of this globulin, along with the HMW glutenin, likely occurred after the divergence of the wheat and barley genomes, since the barley orthologous region contains only one copy each of the globulin and D-hordein (HMW-glutenin orthologue) gene . Given the recent report that rye possesses two copies of the HMW-glutenin genes (De Bustos et al, 2001), the duplication event might happen before the separation of the wheat and rye from their common ancestor, which is estimated to be about 7 million years .…”

Section: Storage Protein Genesmentioning

confidence: 99%

Dynamics of the evolution of orthologous and paralogous portions of a complex locus region in two genomes of allopolyploid wheat

Kong

You

et al. 2004

Plant Mol Biol

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“…In barley, the D-hordeins, which are encoded by the locus Hor3 on chromosome 5, have been postulated to be structurally related to HMW glutenin subunits from wheat (Halford et al 1992b). In rye, the Glu-R1 locus has recently been shown to resemble the Glu-1 locus in specifying one x-and one y-type HMW glutenin subunit during seed development (De Bustos et al 2001). In Elytrigia elongata and Dasypyrum villosum, HMW glutenin subunits are found to be encoded by genes located on chromosome 1E and 1V, respectively (Lawrence and Shepherd 1981;Dvorµk et al 1986;Montebove et al 1987;De Pace et al 1988;Blanco et al 1991;Zhong and Qualset 1993).…”

Section: Introductionmentioning

confidence: 99%

Analysis of HMW glutenin subunits and their coding sequences in two diploid Aegilops species

et al. 2003

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Considerable progress has been made in understanding the structure, function and genetic regulation of high-molecular-weight (HMW) glutenin subunits in hexaploid wheat. In contrast, less is known about these types of proteins in wheat related species. In this paper, we report the analysis of HMW glutenin subunits and their coding sequences in two diploid Aegilops species, Aegilops umbellulata (UU) and Aegilops caudata (CC). SDS-PAGE analysis demonstrated that, for each of the four Ae. umbellulata accessions, there were two HMW glutenin subunits (designated here as 1Ux and 1Uy) with electrophoretic mobilities comparable to those of the x- and y-type subunits encoded by the Glu-D1 locus, respectively. In our previous study involving multiple accessions of Ae. caudata, two HMW glutenin subunits (designated as 1Cx and 1Cy) with electrophoretic mobilities similar to those of the subunits controlled by the Glu-D1 locus were also detected. These results indicate that the U genome of Ae. umbellulata and the C genome of Ae. caudata encode HMW glutenin subunits that may be structurally similar to those specified by the D genome. The complete open reading frames (ORFs) coding for x- and y-type HMW glutenin subunits in the two diploid species were cloned and sequenced. Analysis of deduced amino acid sequences revealed that the primary structures of the x- and y-type HMW glutenin subunits of the two Aegilops species were similar to those of previously published HMW glutenin subunits. Bacterial expression of modified ORFs, in which the coding sequence for the signal peptide was removed, gave rise to proteins with electrophoretic mobilities identical to those of HMW glutenin subunits extracted from seeds, indicating that upon seed maturation the signal peptide is removed from the HMW glutenin subunit in the two species. Phylogenetic analysis showed that 1Ux and 1Cx subunits were most closely related to the 1Dx type subunit encoded by the Glu-D1 locus. The 1Uy subunit possessed a higher level of homology to the 1Dy-type subunit compared with the 1Cy subunit. In conclusion, our study suggests that the Glu-U1 locus of Ae. umbellulata and the Glu-C1 locus of Ae. caudata specify the expression of HMW glutenin subunits in a manner similar to the Glu-D1 locus. Consequently, HMW glutenin subunits from the two diploid species may have potential value in improving the processing properties of hexaploid wheat varieties.

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Characterisation of two gene subunits on the 1R chromosome of rye as orthologs of each of the Glu-1 genes of hexaploid wheat

Cited by 36 publications

References 22 publications

Rapid Genome Evolution Revealed by Comparative Sequence Analysis of Orthologous Regions from Four Triticeae Genomes

Rapid Genome Evolution Revealed by Comparative Sequence Analysis of Orthologous Regions from Four Triticeae Genomes

Dynamics of the evolution of orthologous and paralogous portions of a complex locus region in two genomes of allopolyploid wheat

Analysis of HMW glutenin subunits and their coding sequences in two diploid Aegilops species

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