In this study, the aim was to characterize the physicochemical properties and some bioactive compounds of expeller‐pressed oils of five registered poppy seed varieties (TMO–1, Ofis–8, Ofis–96, Ofis–95, Ofis–3) grown in Turkey. The amounts of total carotenoids, chlorophylls, phenols, and antioxidant activities of oils ranged between 0.08–0.24 mg 100 g−1, 0.03–9.04 mg pheophytin a kg−1, 3.41–8.57 mg gallic acid equivalent 100 g−1, and 5.60–7.33 mM Trolox equivalent 100 g−1, respectively. The most abundant fatty acid in poppy seed oils was linoleic acid (69.85–74.02%), followed by oleic acid (13.98–16.99%), and palmitic acid (8.51–9.75%). In addition, poppy seed oils were rich in β–sitosterol (133.47–153.42 mg 100 g−1), campesterol (45.36–58.60 mg 100 g−1), and δ–5–avenasterol (28.21–39.40 mg 100 g−1). High amounts of γ–tocopherol and α–tocopherol were detected. This research is the first study, which identified and quantified the polyphenol, β–carotene, and lutein compounds of expeller–pressed poppy seed oils by HPLC. Tyrosol, apigenin, syringic acid, 3–hydroxytyrosol, luteolin, p–coumaric acid, quercetin, ferulic acid, sinapic acid, and veratric acid were detected in expeller‐pressed poppy seed oils.