Characterisation of volatile organic compounds in stemwood using solid-phase microextraction

Abstract

Solid-phase microextraction (SPME), hydrodistillation and dynamic headspace combined with GC and GC-MS were applied and compared for the analysis of volatile organic compounds (VOCs) from coniferous wood. The SPME conditions (type of fibre, size of wood sample, temperature and exposure time) were optimised, and more than 100 VOCs and semi-volatile compounds extracted and identified from the sapwood and heartwood of Norway spruce (Picea abies). The total number of mono- and sesquiterpenes eluted and identified was similar for the SPME and hydrodistillation methods, but more semi-volatile compounds were released by hydrodistillation. By applying dynamic headspace at room temperature, it was possible to analyse only the most volatile compounds. The qualitative composition of VOCs was similar in spruce sapwood and heartwood, although Z-beta-ocimene occurred only in sapwood while fenchol was present only in heartwood. SPME sampling coupled with GC, applied here to the analysis of VOCs released from stemwood of firs for the first time, is a convenient, sensitive, fast, solvent-free and simple method for the determination of wood volatiles. The technique requires much smaller sample amounts compared with hydrodistillation, and the total amount of VOCs extracted and identified is higher than that obtained by hydrodistillation or dynamic headspace. The relative ratios of the main mono- and sesquiterpenes and -terpenoids were similar using the SPME-GC and hydrodistillation methods.

Diversity of the Volatile Organic Compounds Emitted by 55 Species of Tropical Trees: a Survey in French Guiana

“…Table 1 lists the sampled species and sampling size. Sampling a large number of species requires a rapid technique and appropriate storage for subsequent analysis (Wajs et al 2006). For each tree, we cut about 20 mg of tissue from a young leaf.…”

Optimizing the attractiveness of pheromone baits used for trapping the four‐eyed spruce bark beetle Polygraphus poligraphus

“…But it was not possible to draw conclusions as the total catches were very low that year (Table S3). α-Terpineol has previously been detected in small quantities in Norway spruce (Wajs et al, 2006) and has been found in SPME samples from stem sections with boring P. poligraphus (Rahmani et al, 2015). It is possible that the effects of α-terpineol combined with (−)-terpinen-4-ol could be dependent on the release rate or ratio between the compounds.…”

“…It is possible that such effects could also exist in P. poligraphus . Several compounds which are emitted from spruce trees when P. poligraphus is boring through the bark have been identified but so far not tested for attraction on the beetles (Rahmani et al, ; Wajs, Pranovich, Reunanen, Willfor, & Holmbom, ; R. Rahmani and L. Viklund, unpublished results). It has also been shown that bark beetles of the same genus often have overlapping pheromone components (Blomquist et al, ), and therefore, 3‐methyl‐3‐buten‐1‐ol, the pheromone of Polygraphus rufipennis (Bowers, Gries, Borden, & Pierce, ), was considered interesting for our studies.…”

“…It is possible that such effects could also exist in P. poligraphus. Several compounds which are emitted from spruce trees when P. poligraphus is boring through the bark have been identified but so far not tested for attraction on the beetles (Rahmani et al, 2015;Wajs, Pranovich, Reunanen, Willfor, & Holmbom, 2006;R. Rahmani and L. Viklund, unpublished results).…”

Effect of carrot (Daucus carota) antifreeze proteins on texture properties of frozen dough and volatile compounds of crumb

“…The volatile compounds were absorbed by SPME sampling method using a 75 mm CAR/PDMS absorbing pin (Custodio, Serra, Nogueira, Goncalves, & Romano, 2006;Mallouchos, Komaitis, Koutinas, & Kanellaki, 2002;Wajs, Pranovich, Reunanen, Willfor, & Holmbom, 2006). Specifically, 3 g crumb of sample was sealed in a 20 ml glass vial and stirred at 50 1C for 35 min.…”