Characterisation of water-soluble proanthocyanidins of Pyracantha fortuneana fruit and their improvement in cell bioavailable antioxidant activity of quercetin

Combination of selenium-enriched green tea polysaccharides and Huo-ji polysaccharides synergistically enhances antioxidant and immune activity in mice

“…These studies indicate that Se‐GTP or HJP may enhance the immune function in mice. These findings are consistent with results from our group as well as other groups …”

“…The remaining cells were suspended at a density of 2 × 10 5 cells mL −1 in RPMI‐1640 medium supplemented with 10% fetal bovine serum (FBS). Splenocytes (100 μL well −1 ) were seeded onto a 96‐well plate in the presence of ConA (8 µg mL −1 ) . Splenocytes were cultured in 5% CO 2 at 37 °C for 72 h, and 10 µL of Cell Counting Kit‐8 (CCK‐8) (Dojindo Laboratories, Kumamoto, Japan) was then added to each well.…”

“…Splenocytes were lysed, and nuclear fractions were prepared as described previously . The protein levels of Nrf2 in splenocytes were assayed with western blot.…”

“…Splenocytes (100 L well −1 ) were seeded onto a 96-well plate in the presence of ConA (8 μg mL −1 ). 32 Splenocytes were cultured in 5% CO 2 at 37 ∘ C for 72 h, and 10 μL of Cell Counting Kit-8 (CCK-8) (Dojindo Laboratories, Kumamoto, Japan) was then added to each well. The plate was incubated for another 2.5 h. Finally, the absorbance was measured at 450 nm.…”

Identification of phenolics in litchi and evaluation of anticancer cell proliferation activity and intracellular antioxidant activity

“…The difference between PBS wash protocol and no PBS wash protocol revealed the cellular uptake of tested compounds. Previous report indicated that quercetin was the 22 most efficient compound with 100% uptake in HepG 2 cells, exhibiting the highest CAA value [22]. In the present work, the uptake rate of cinnamtannin B1 was only 16.43%, much lower than that of quercetin.…”