Characteristic fingerprint analysis of Dendrobium huoshanense by ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry

Wu, Chengfeng; Gui, Su; Huang, Yuechun; Dai, Yafeng; Shun, Qing-Sheng; Tao, Shengchang; Wei, Gang

doi:10.1039/c6ay00232c

Cited by 22 publications

(21 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although nine flavonoids in D. huoshanense were identified using a UHPLC‐ESI‐MS n method in our previous study , these compounds were not sufficient for the quality control of D. huoshanense . Therefore, in this study, we identified additional flavonoids and identified components specific to D. huoshanense by TLC, HPLC and HPLC–ESI‐MS n .…”

Section: Resultsmentioning

confidence: 80%

“…officinale from Zhejiang province and found that they mainly contain flavonoid C‐glycosides. Additionally, our studies on D. officinale stems and leaves showed that D. officinale could be classified into three categories based on the flavonoids present . Chang et al.…”

Section: Introductionmentioning

confidence: 79%

“… isolated four flavonoids from D. huoshanense , and each had a molecular weight of 710. In D. huoshanense , we identified flavonoids including vicenin‐2, schaftoside, isoschaftoside, and apigenin‐6,8‐di‐C‐β‐D‐arabinoside . In view of the above results, using flavonoids for the quality control of D. huoshanense may be possible.…”

Section: Introductionmentioning

confidence: 89%

“…Many studies on its chemical composition have revealed that D. huoshanense contains polysaccharides , flavonoids , alkaloids , and other compounds. However, the unique growth environment (it can only grow in Huoshan County) of D. huoshanense and its slow growth rate are the main reasons why wild D. huoshanense is scarce and the plant is endangered . Additionally, because of its significant biological effects, the demand for D. huoshanense on the herbal market is substantial.…”

Section: Introductionmentioning

confidence: 99%

“…Zhou et al [12] studied the flavonoids in the leaves of D. officinale from Zhejiang province and found that they mainly contain flavonoid C-glycosides. Additionally, our studies on D. officinale stems and leaves showed that D. officinale could be classified into three categories based on the flavonoids present [9,[13][14][15]. Chang et al [4] isolated four flavonoids from D. huoshanense, and each had a molecular weight of 710.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Identification of flavonoids in Dendrobium huoshanense and comparison with those in allied species of Dendrobium by TLC, HPLC and HPLC coupled with electrospray ionization multi‐stage tandem MS analyses

et al. 2019

Self Cite

View full text Add to dashboard Cite

Dendrobium huoshanense, a unique species in the genus Orchidaceae, is only found in China and is known as “mihu”. Due to the lack of quality control, the use of D. huoshanense in the herbal market has been limited. In this study, methods based on thin‐layer chromatography, high‐performance liquid chromatography and high‐performance liquid chromatography coupled with electrospray ionization multi‐stage tandem mass spectrometry were used to identify the flavonoids in D. huoshanense and distinguish this species from other Dendrobium species. Using thin‐layer chromatography, a characteristic band was observed for D. huoshanense, and this band was absent from the thin‐layer chromatography plates of other Dendrobium species. Then, using high‐performance liquid chromatography, nine peaks of flavonoids were observed in the chromatograms of ten batches of D. huoshanense. Ultimately, 22 flavonoids in D. huoshanense were identified by multi‐stage tandem mass spectrometry, and 11 of these compounds are being reported from D. huoshanense for the first time. In addition, two compounds both with molecular weights of 710, were identified as being unique to D. huoshanense; one of these compounds, apigenin‐6‐C‐α‐L‐rhamnosyl‐(1→2)‐β‐D‐glucoside‐8‐C‐α‐L‐arabinoside, was proven to be responsible for the characteristic thin‐layer chromatography band of D. huoshanense. These analysis methods can be applied for the identification and quality control of D. Huoshanense.

show abstract

Section: Resultsmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Identification of flavonoids in Dendrobium huoshanense and comparison with those in allied species of Dendrobium by TLC, HPLC and HPLC coupled with electrospray ionization multi‐stage tandem MS analyses

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

Comparative analysis of chemical constituents by HPLC–ESI–MSⁿ and antioxidant activities of Dendrobium huoshanense and Dendrobium officinale

Wan

Gong

Wang

et al. 2021

Biomedical Chromatography

View full text Add to dashboard Cite

Dendrobium huoshanense is a Chinese medicinal herb that has high quality and excellent efficacy. However, the chemical basis of its activity is still unclear. Of note, Dendrobium officinale is the most widely utilized among the Dendrobium species. Therefore, the current study systematically investigated the chemical constituents of methanolic extracts and different polar fractions of aqueous extracts from the two herbs by HPLC–ESI–MSn, and then compared in vitro antioxidant activities of their five different polar extracts. Consequently, 61 and 49 compounds were identified from D. huoshanense and D. officinale, respectively, of which 43 compounds were common to both species. In addition, 17 out of 22 different compounds were identified only in D. huoshanense. Moreover, the peak areas of some shared identical compounds of D. huoshanense were significantly larger than that of D. officinale. In vitro antioxidant evaluation results showed that the n‐BuOH‐soluble fraction of the two herbs exhibited remarkable antioxidant activities. Furthermore, the antioxidant activities of different fractions of D. huoshanense were separately superior to that of D. officinale, which may be attributed to its variable and high contents of flavonoids, bibenzyls and phenanthrenes. These results provide the evidence for the high quality and efficacy of D. huoshanense.

show abstract

Dendrobium huoshanense Polysaccharides Prevent Inflammatory Response of Ulcerative Colitis Rat through Inhibiting the NF‐κB Signaling Pathway

Huang

et al. 2021

Chemistry & Biodiversity

View full text Add to dashboard Cite

The polysaccharides of the Chinese herbal medicine Dendrobium huoshanense exhibit anti-inflammatory effects in multiple organs through regulating the immune responses. In the present study, we constructed ulcerative colitis (UC) model rats using dextran sulfate sodium to investigate the anti-inflammatory effects of D. huoshanense polysaccharides (DHP). After oral administration of DHP for two weeks, the indices of UC symptoms, including the ratio of colon weight to length, Disease Activity Index (DAI), and Colon Mucosal Damage Index (CMDI), all decreased significantly compared with the UC model group. The histological sections also revealed better cell orders in DHP treatments than in the UC model rats. Moreover, in treatment with high dose of DHP (200 mg/kg), the treatment efficacy arrived the similar levels to those in the treatment with 300 mg/kg sulfasalazine, which is a typical medicine to treat UC. These results indicated that DHP has a high efficacy to treat UC in model rats. Furthermore, serum levels of interleukin-1β, tumor necrosis factor-α, interleukin-17, and transforming growth factor-β were assessed using the enzyme linked immunosorbent assay (ELISA) method, and the levels of nuclear factor-кB in colon tissue sections were determined using the immunohistochemical method. The results showed that all these indices decreased significantly after administration of DHP in UC model rats, which might be the mechanisms underlying the DHP-suppressed UC inflammation. Overall, this study indicated that DHP might be directly used to treat UC and is a promising source to develop novel drugs against UC.

show abstract

Characteristic fingerprint analysis of Dendrobium huoshanense by ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry

Abstract: A new method for the quality control of genuine Dendrobium huoshanense, which is rare and costly.

Cited by 22 publications

References 19 publications

Identification of flavonoids in Dendrobium huoshanense and comparison with those in allied species of Dendrobium by TLC, HPLC and HPLC coupled with electrospray ionization multi‐stage tandem MS analyses

Identification of flavonoids in Dendrobium huoshanense and comparison with those in allied species of Dendrobium by TLC, HPLC and HPLC coupled with electrospray ionization multi‐stage tandem MS analyses

Comparative analysis of chemical constituents by HPLC–ESI–MSⁿ and antioxidant activities of Dendrobium huoshanense and Dendrobium officinale

Dendrobium huoshanense Polysaccharides Prevent Inflammatory Response of Ulcerative Colitis Rat through Inhibiting the NF‐κB Signaling Pathway

Contact Info

Product

Resources

About

Characteristic fingerprint analysis of Dendrobium huoshanense by ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry

Abstract: A new method for the quality control of genuine Dendrobium huoshanense, which is rare and costly.

Cited by 22 publications

References 19 publications

Identification of flavonoids in Dendrobium huoshanense and comparison with those in allied species of Dendrobium by TLC, HPLC and HPLC coupled with electrospray ionization multi‐stage tandem MS analyses

Identification of flavonoids in Dendrobium huoshanense and comparison with those in allied species of Dendrobium by TLC, HPLC and HPLC coupled with electrospray ionization multi‐stage tandem MS analyses

Comparative analysis of chemical constituents by HPLC–ESI–MSn and antioxidant activities of Dendrobium huoshanense and Dendrobium officinale

Dendrobium huoshanense Polysaccharides Prevent Inflammatory Response of Ulcerative Colitis Rat through Inhibiting the NF‐κB Signaling Pathway

Contact Info

Product

Resources

About

Comparative analysis of chemical constituents by HPLC–ESI–MSⁿ and antioxidant activities of Dendrobium huoshanense and Dendrobium officinale