Characteristic responses of a phospholipid molecular layer to polyols

Nakata, Satoshi; Deguchi, Ayano; Seki, Yota; Furuta, Miyuki; Fukuhara, Koichi; Nishihara, Sadafumi; Inoue, Katsuya; Kumazawa, Noriyuki; Mashiko, S.; Fujihira, Shota; Goto, Makiko; Denda, Mitsuhiro

doi:10.1016/j.colsurfb.2015.09.035

Cited by 22 publications

(2 citation statements)

References 30 publications

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“…The high encapsulation efficiency of liposomes was in direct proportion with high levels of lipid, due to the more internal volume available for materials to be encapsulated. The available internal volume was a consequence of the larger population of liposomes formed during reconstitution with regard to more lipids [49].…”

Section: Resultsmentioning

confidence: 99%

Optimization of Polyarginine-Conjugated PEG Lipid Grafted Proliposome Formulation for Enhanced Cellular Association of a Protein Drug

et al. 2019

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The purpose of this study was to develop an oral proliposomal powder of protein using poly-l-arginine-conjugated 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-poly(ethylene glycol) (DSPE-PEG) (PLD) for enhancing cellular association upon reconstitution and to compare its effects with a non-grafted and PEGylated formulation. Cationic proliposome (CATL), PLD-grafted CATL (PLD-CATL), PEGylated CATL (PEG CATL), and PLD grafted-PEG CATL (PLD-PEG CATL) were prepared and compared. Successful conjugation between poly-l-arginine and DSPE-PEG was confirmed by 1H NMR and FT-IR. PLD was successfully grafted onto the proliposomal powder during the slurry process. Although reconstituted liposomal sizes of CATL and PLD-CATL were increased by agglomeration, PEGylation reduced the agglomeration and increased the encapsulation. The viabilities of cells treated with both CATL and PLD-CATL formulations were low but increased following PEGylation. With regard to cellular association, PLD-CATL enhanced cellular association/uptake more rapidly than did CATL. Upon PEGylation, PEG CATL showed a lower level of cellular association/uptake compared with CATL while PLD-PEG CATL did not exhibit the rapid cellular association/uptake as seen with PLD-CATL. However, PLD-PEG CATL still enhanced the higher cellular association/uptake than PEG CATL did without PLD. In conclusion, proliposomes with PLD could accelerate cellular association/uptake but also caused high cellular toxicity. PEGylation reduced cellular toxicity and also changed the cellular association pattern of the PLD formulation.

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Section: Resultsmentioning

confidence: 99%

Optimization of Polyarginine-Conjugated PEG Lipid Grafted Proliposome Formulation for Enhanced Cellular Association of a Protein Drug

et al. 2019

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“…Additionally, opposite to sugars that have to be dissolved in the dispersion media of nanovesicles before the bilayers are closed (affecting further manufacturing process such as filter clogging during extrusion), glycerol can be added to already formed nanovesicle suspensions. Additionally, glycerolstably packaged between lipid molecules in the bilayer, establishing hydrogen bonding with phosphate groups in phospholipids, increasing the area occupied per phospholipid molecule [50]. Finally, glycerol is frequently used in topical dermatological preparations because of its moisturizing and smoothing effects, and has been used alone or combined with other agents to lyophilize liposomes [51,52].…”

Section: Discussionmentioning

confidence: 99%

Topical vaccination with super-stable ready to use nanovesicles

Caimi

Parra

Farias

et al. 2017

Colloids and Surfaces B: Biointerfaces

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Controlling the antibacterial activity of surfactants by hydrotrope addition

Kurose,

Sato,

Yano

et al. 2024

J Surfact & Detergents

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Hydrotropes are amphiphiles that improve the water solubility of hydrophobic substances. In this study, we systematically evaluated the effects of eight hydrotropes on the antibacterial activity of fatty acids solubilized in a micellar system. The antibacterial activity was evaluated using minimum inhibitory concentration (MIC) as an indicator, and CMC was calculated for interfacial properties based on the measurement of the surface tension and the solubilization behavior of the oil‐soluble dye. The solubilization of lauric acid (12:0 FA) in the micelles of nonionic surfactants increased and decreased the antibacterial activities of the surfactants against Staphylococcus aureus (S. aureus) and Staphylococcus epidermidis, respectively. The addition of the hydrotropes decreased the antibacterial activity of the surfactants against both Staphylococci. However, on introducing small amounts of 12:0 FA, the surfactants exhibited selective antibacterial activity against S. aureus. These antibacterial phenomena were observed at concentrations above the critical micelle concentration of the surfactants. These findings suggest that the addition of hydrotropes affects the interfacial properties and antibacterial activity of micellar systems containing antibacterial ingredients.

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Characteristic responses of a phospholipid molecular layer to polyols

Cited by 22 publications

References 30 publications

Optimization of Polyarginine-Conjugated PEG Lipid Grafted Proliposome Formulation for Enhanced Cellular Association of a Protein Drug

Optimization of Polyarginine-Conjugated PEG Lipid Grafted Proliposome Formulation for Enhanced Cellular Association of a Protein Drug

Topical vaccination with super-stable ready to use nanovesicles

Controlling the antibacterial activity of surfactants by hydrotrope addition

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