Characteristics and functions of lipid droplets and associated proteins in enterocytes

Beilstein, Frauke; Carrière, Véronique; Leturque, Armelle; Demignot, Sylvie

doi:10.1016/j.yexcr.2015.09.018

Cited by 65 publications

(62 citation statements)

References 62 publications

(115 reference statements)

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“…In addition, specific isoforms of enzymes that activate FAs to form acyl-CoAs for incorporation into TAG (ACSL1, ACSL3, and ACSL4) have been identified on CLDs in several cell types (reviewed in [118]). In enterocytes, TAG synthesis enzymes that have been identified on CLDs are ACSL3, ACSL4, ACSL5, GPAT3 and MGAT2 in mice and humans (reviewed in [45, 119]). Of these, ACSL3 [120], ACSL5 [97], and GPAT3 [45] have been validated by confocal or electron microscopy to be present on CLDs in enterocytes.…”

Section: Chylomicron (Cm) and Cytoplasmic Lipid Droplet (Cld) Syntmentioning

confidence: 99%

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Recent discoveries on absorption of dietary fat: Presence, synthesis, and metabolism of cytoplasmic lipid droplets within enterocytes

D’Aquila

Hung

Carreiro

et al. 2016

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

115

122

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Dietary fat provides essential nutrients, contributes to energy balance, and regulates blood lipid concentrations. These functions are important to health, but can also become dysregulated and contribute to diseases such as obesity, diabetes, cardiovascular disease, and cancer. Within enterocytes, the digestive products of dietary fat are re-synthesized into triacylglycerol, which is either secreted on chylomicrons or stored within cytoplasmic lipid droplets (CLDs). CLDs were originally thought to be inert stores of neutral lipids, but are now recognized as dynamic organelles function in multiple cellular processes in addition to lipid metabolism. This review will highlight recent discoveries related to dietary fat absorption with an emphasis on the presence, synthesis, and metabolism of cytoplasmic lipid droplets within this process.

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Section: Chylomicron (Cm) and Cytoplasmic Lipid Droplet (Cld) Syntmentioning

confidence: 99%

“…The proteomes of isolated CLDs derived from Caco-2 cells and mouse enterocytes have been analyzed using a tandem mass spectrometery approach [97, 120, 125] and have been previously reviewed [119]. These studies identified both known and novel CLD associated proteins.…”

Section: Identification Of Novel Cytoplasmic Lipid Droplet (Cld) Amentioning

confidence: 99%

Recent discoveries on absorption of dietary fat: Presence, synthesis, and metabolism of cytoplasmic lipid droplets within enterocytes

D’Aquila

Hung

Carreiro

et al. 2016

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

115

122

View full text Add to dashboard Cite

show abstract

“…DHSRS3 is localized in the endoplasmic reticulum (ER) and lipid droplets, both of which are storage vesicles containing lipid, such as cholesterol and retinol esters [48]. Additionally, overexpression of DHRS3 in U2OS cells increases lipid droplet accumulation (Figure 1B) [26].…”

Section: Regulation Of Lipid Metabolism By Wild-type P53mentioning

confidence: 99%

p53 as a Regulator of Lipid Metabolism in Cancer

Parrales

Iwakuma

2016

IJMS

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Enhanced proliferation and survival are common features of cancer cells. Cancer cells are metabolically reprogrammed which aids in their survival in nutrient-poor environments. Indeed, changes in metabolism of glucose and glutamine are essential for tumor progression. Thus, metabolic reprogramming is now well accepted as a hallmark of cancer. Recent findings suggest that reprogramming of lipid metabolism also occurs in cancer cells, since lipids are used for biosynthesis of membranes, post-translational modifications, second messengers for signal transduction, and as a source of energy during nutrient deprivation. The tumor suppressor p53 is a transcription factor that controls the expression of proteins involved in cell cycle arrest, DNA repair, apoptosis, and senescence. p53 also regulates cellular metabolism, which appears to play a key role in its tumor suppressive activities. In this review article, we summarize non-canonical functions of wild-type and mutant p53 on lipid metabolism and discuss their association with cancer progression.

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“…The bulk of dietary PUFAs is in the form of neutral fat or triglyceride and is well absorbed in the small intestine via emulsification, hydrolysis, and micelle formation (15). Following lipoprotein transport, PUFAs are incorporated into cell membrane glycerophospholipids by a remodeling pathway (Lands' cycle) to generate membrane asymmetry and diversity (194).…”

Section: N-3 Pufa and Transcriptional Machinerymentioning

confidence: 99%

Nutrient-Gene Interaction in Colon Cancer, from the Membrane to Cellular Physiology

et al. 2016

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The International Agency for Research on Cancer recently released an assessment classifying red and processed meat as “carcinogenic to humans” on the basis of the positive association between increased consumption and risk for colorectal cancer. Diet, however, can also decrease the risk for colorectal cancer and be used as a chemopreventive strategy. Bioactive dietary molecules, such as n-3 polyunsaturated fatty acids, curcumin, and fermentable fiber, have been proposed to exert chemoprotective effects, and their molecular mechanisms have been the focus of research in the dietary/chemoprevention field. Using these bioactives as examples, this review surveys the proposed mechanisms by which they exert their effects, from the nucleus to the cellular membrane. In addition, we discuss emerging technologies involving the culturing of colonic organoids to study the physiological effects of dietary bioactives. Finally, we address future challenges to the field regarding the identification of additional molecular mechanisms and other bioactive dietary molecules that can be utilized in our fight to reduce the incidence of colorectal cancer.

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Characteristics and functions of lipid droplets and associated proteins in enterocytes

Cited by 65 publications

References 62 publications

Recent discoveries on absorption of dietary fat: Presence, synthesis, and metabolism of cytoplasmic lipid droplets within enterocytes

Recent discoveries on absorption of dietary fat: Presence, synthesis, and metabolism of cytoplasmic lipid droplets within enterocytes

p53 as a Regulator of Lipid Metabolism in Cancer

Nutrient-Gene Interaction in Colon Cancer, from the Membrane to Cellular Physiology

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