Characteristics and Kinetic Properties of L-Rhamnose Isomerase from &lt;i&gt;Bacillus Subtilis&lt;/i&gt; by Isothermal Titration Calorimetry for the Production of D-Allose

Bai, Wei; Shen, Jie; Zhu, Yueming; Men, Yan; Sun, Yi; Ma, Yanhe

doi:10.3136/fstr.21.13

Cited by 28 publications

(13 citation statements)

References 22 publications

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“…Several enzymes have been characterized to be strictly metal ion dependent, exhibiting no enzymatic activity in the absence of metal ions, such as L‐RI from B. subtilis str. 168, l ‐arabinose isomerase from Geobacillus stearothermophilus DSM 22 and d ‐psicose 3‐epimerases from Clostridium species strains . A comparison of the enzymatic properties of L‐RIs from various microorganisms is shown in Table .…”

Section: Resultsmentioning

confidence: 99%

“…Although the C. obsidiansis L‐RI is not particularly thermostable, it is better than L‐RIs from other microorganisms, such as B. subtilis str. 168 (6 h, 65 °C), B. halodurans ATCC BAA‐125 (0.42 h, 70 °C), T. composti KWC4 (0.46 h, 75 °C) and B. pallidus Y25 (1 h, 65 °C) (Table ).…”

Section: Resultsmentioning

confidence: 99%

“…So far, L‐RIs have been isolated and characterized from Escherichia coli , Pseudomonas stutzeri , Bacillus pallidus Y25, Thermoanaerobacterium saccharolyticum NTOU1, Thermotoga maritima ATCC 43589, Caldicellulosiruptor saccharolyticus ATCC 43494, Mesorhizobium loti Tono, Bacillus halodurans ATCC BAA‐125, Dictyoglomus turgidum DSMZ 6724, Bacillus subtilis ATCC 23857, Bacillus subtilis str. 168 and Thermobacillus composti KWC4 . Among these, three cloned and characterized L‐RIs have excellent thermostability, including C. saccharolyticus ATCC 43494 L‐RI, T. maritima ATCC 43589 L‐RI and D. turgidum DSMZ 6724 L‐RI .…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a thermostable recombinant l‐rhamnose isomerase from Caldicellulosiruptor obsidiansis OB47 and its application for the production of l‐fructose and l‐rhamnulose

Chen

Zhang

et al. 2017

J Sci Food Agric

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of a thermostable recombinant l‐rhamnose isomerase from Caldicellulosiruptor obsidiansis OB47 and its application for the production of l‐fructose and l‐rhamnulose

Chen

Zhang

et al. 2017

J Sci Food Agric

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“…Also, it is functionally significant in pharmaceutical as well as nutritional industries for its applications in reduction of calories and sweeteners . L‐rhamnose is also characterized to reduce cholesterogenesis and decrease the synthesis of triacylglecerides fatty acids , by increasing serum propionate in humans which results in changes in colonic productions leading to modification in mechanisms of blood lipids . Importantly, L‐rhamnose is reported to possess anti‐ageing properties by inhibiting microbial growth in age‐related cytotoxic compounds .…”

Section: Introductionmentioning

confidence: 99%

L‐rhamnose induces browning in 3T3‐L1 white adipocytes and activates HIB1B brown adipocytes

et al. 2018

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Induction of the brown adipocyte-like phenotype in white adipocytes (browning) is considered as a novel strategy to fight obesity due to the ability of brown adipocytes to increase energy expenditure. Here, we report that L-rhamnose induced browning by elevating expression levels of beige-specific marker genes, including Cd137, Cited1, Tbx1, Prdm16, Tmem26, and Ucp1, in 3T3-L1 adipocytes. Moreover, L-rhamnose markedly elevated expression levels of proteins involved in thermogenesis both in 3T3-L1 white and HIB1B brown adipocytes. L-rhamnose treatment in 3T3-L1 adipocytes also significantly elevated protein levels of p-HSL, p-AMPK, ACOX, and CPT1 as well as reduced levels of ACC, FAS, C/EBPα, and PPARγ, suggesting its possible role in enhancement of lipolysis and lipid catabolism as well as reduced adipogenesis and lipogenesis, respectively. The quick technique of efficient molecular docking provided insight into the strong binding of L-rhamnose to the fat-digesting glycine residue of β -adrenergic receptor (AR), indicating strong involvement of L-rhamnose in fat metabolism. Further examination of the molecular mechanism of L-rhamnose revealed that it induced browning of 3T3-L1 adipocytes via coordination of multiple signaling pathways through β -AR, SIRT1, PKA, and p-38. To the best of our knowledge, this is the first study to demonstrate that L-rhamnose plays multiple modulatory roles in the induction of white fat browning, activation of brown adipocytes, as well as promotion of lipid metabolism, thereby demonstrating its therapeutic potential for treatment of obesity. © 2018 IUBMB Life, 70(6):563-573, 2018.

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“…The second reaction, the conversion of D-fructose to D-allulose, can be catalyzed by D-tagatose 3-epimerase or D-allulose 3-epimerase. D-Allose is produced in the final step by the conversion of Dallulose by L-rhamnose isomerase from Clostridium stercorarium (Seo et al, 2018), Thermobacillus composti (Xu et al, 2017), Bacillus subtilis (Bai et al, 2015), or Pseudomonas sp. (Bhuiyan et al, 1998); by ribose-5-phosphate isomerase from Clostridium thermocellum (Park et al, 2007b) or Thermotoga lettingae (Feng et al, 2013); or by galactose 6-phosphate isomerase from Lactococcus lactis (Park et al, 2007a).…”

Section: Introductionmentioning

confidence: 99%

Production of D-Allose From D-Allulose Using Commercial Immobilized Glucose Isomerase

Choi

Shin

Kim

et al. 2021

Front. Bioeng. Biotechnol.

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Rare sugars are regarded as functional biological materials due to their potential applications as low-calorie sweeteners, antioxidants, nucleoside analogs, and immunosuppressants. D-Allose is a rare sugar that has attracted substantial attention in recent years, owing to its pharmaceutical activities, but it is still not widely available. To address this limitation, we continuously produced D-allose from D-allulose using a packed bed reactor with commercial glucose isomerase (Sweetzyme IT). The optimal conditions for D-allose production were determined to be pH 8.0 and 60°C, with 500 g/L D-allulose as a substrate at a dilution rate of 0.24/h. Using these optimum conditions, the commercial glucose isomerase produced an average of 150 g/L D-allose over 20 days, with a productivity of 36 g/L/h and a conversion yield of 30%. This is the first report of the successful continuous production of D-allose from D-allulose by commercial glucose isomerase using a packed bed reactor, which can potentially provide a continuous production system for industrial applications of D-allose.

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Characteristics and Kinetic Properties of L-Rhamnose Isomerase from <i>Bacillus Subtilis</i> by Isothermal Titration Calorimetry for the Production of D-Allose

Cited by 28 publications

References 22 publications

Characterization of a thermostable recombinant l‐rhamnose isomerase from Caldicellulosiruptor obsidiansis OB47 and its application for the production of l‐fructose and l‐rhamnulose

Characterization of a thermostable recombinant l‐rhamnose isomerase from Caldicellulosiruptor obsidiansis OB47 and its application for the production of l‐fructose and l‐rhamnulose

L‐rhamnose induces browning in 3T3‐L1 white adipocytes and activates HIB1B brown adipocytes

Production of D-Allose From D-Allulose Using Commercial Immobilized Glucose Isomerase

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