Characteristics of 4-Day Infradian Biorhythms in Mature Male Wistar Rats after Pinealectomy

Diatroptov, M. E.; Слесарев, С. М.; Слесарева, Е. В.

doi:10.1007/s10517-017-3749-3

Cited by 9 publications

(8 citation statements)

References 5 publications

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“…This makes the effect of melatonin more diverse and complicated on neuroendocrine, especially in many inconsistent results on the HPA axis [52]. For example, the reduction of melatonin caused by the pinealectomy has no significant effect on the CORT secretion in male rats [54], while the secretion in female rats is upregulated [55]. In the present study, the deficit of melatonin due to the pinealectomy showed a tendency to enhance the basal plasma CORT levels before Dex injection although there was no statistical difference among the four groups.…”

Section: Advances In Polymer Technologymentioning

confidence: 99%

Melatonin-Loaded Nanoparticles for Enhanced Antidepressant Effects and HPA Hormone Modulation

Sun

Ding

et al. 2020

Advances in Polymer Technology

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Background. The present work aims at formulating the melatonin-loaded nanoparticles (MTNPs) exhibiting the controlled-release and pH-sensitivity to repurpose the use of melatonin in the treatment of depressive-like behaviors and hypothalamus-pituitary-adrenal (HPA) axis dysregulation. Methods. MTNPs were characterized for the size, drug incorporation, and in vitro release in the different pH environments. Its merits were in vivo tested on the pinealectomized rats presenting the depressive-like behaviors and the abnormal HPA axis activity by calculating the improvement on saccharin preference, swimming immobility time, and the negative feedback of HPA axis. Results. Results revealed that MTNPs showed nanometer size, 15.77% of drug loading, 33.82% of encapsulation efficiency, the different controlled-release profiles in different pH environments (pH 1.2, pH 6.8, and pH 7.4), more sensitivity release in simulated intestinal fluid (pH 7.4) and blood (pH 6.8), and less sensitivity release in simulated gastric fluid (pH 1.2). Furthermore, MTNPs displayed better antidepressant actions in reducing the immobility time of forced swimming test, increasing the preference for saccharin, and sensitizing the blunt negative feedback of HPA axis, when compared to the free melatonin. Conclusions. The controlled-release nanoparticles is shown to be an effective improvement on the dosage form for melatonin, which is worthy of futuristic and complete evaluation.

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Section: Advances In Polymer Technologymentioning

confidence: 99%

Melatonin-Loaded Nanoparticles for Enhanced Antidepressant Effects and HPA Hormone Modulation

Sun

Ding

et al. 2020

Advances in Polymer Technology

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“…2) does the phase of the rhythm in cell culture comparable with that in vivo? Under in vivo conditions, the phase of the infradian rhythm of the motor activity of animals is observed in antiphase to the 4-day rhythm of mitotic activity (mitotic index) of the esophageal epithelium [7].…”

Section: Introductionmentioning

confidence: 99%

“…Infradian about 4-day uctuations were found in many biological parameters: concentrations of melatonin, glucocorticoid and sex hormones [1][2][3][4][5][6], motor activity [7], immune system [8,9], mitotic activity of cells [10,11]. Based on the facts that 1) 4-day rhythms are preserved during the removal both of the testes and adrenal glands [12], and 2) hibernating hedgehogs with reduced hormonal activity had the stable rhythms of spontaneous short-term arousals [13], it can be argued that the formation of this infradian rhythm is not associated with hormonal regulation.…”

Section: Introductionmentioning

confidence: 99%

“…Based on the facts that 1) 4-day rhythms are preserved during the removal both of the testes and adrenal glands [12], and 2) hibernating hedgehogs with reduced hormonal activity had the stable rhythms of spontaneous short-term arousals [13], it can be argued that the formation of this infradian rhythm is not associated with hormonal regulation. It is interesting that the removal of the pineal gland, which is involved in the formation of circadian rhythms [14], also does not affect the period and phase of the 4-day rhythm of mitotic activity of the rat esophagus epithelium [7]. Probably, the 4-day rhythm is determined by the activation of the sympathetic nervous system, which is indicated by the presence of this rhythm in the dynamics of epinephrine excretion in the urine [2], as well as the whole complex of signs associated with an increase in motor activity, the release of glucocorticoid hormones, suppression of immune reactions, a decrease in proliferative cell activity.…”

Section: Introductionmentioning

confidence: 99%

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About 4-day Rhythm of Proliferative Activity of Fibroblast-like Cell Cultures Depends on the Environmental Factor

Диатроптова

Косырева

Diatroptov

2021

Preprint

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A study of the 4-day rhythm of the proliferative activity of the embryonic fibroblast-like cells in the logarithmic growth phase was carried out. It was shown that in cell cultures obtained on different days from embryos of different ages, the phase of the 4-day rhythm coincides. In vitro the maxima of the proliferative activity were consistent with the minima of the motor activity of mice. Freezing the culture for 2 or 6 days does not cause a shift in the phase of the 4-day rhythm of cell proliferative activity compare with the unfreezing culture. That indicates the existence of an external synchronizer, which determines the 4-day infradian rhythm of the proliferative activity of embryonic cells. Then we daily thawed samples of single L-929 culture of mice fibroblast-like cells for 22 and 17 days and researched the dynamics of its proliferative activity. We also showed 4-day rhythm of the simultaneous increase in the number of cells for all thawed samples. Taking into account that deep freezing of a culture leads to the cessation of all life processes, the fact we obtained indicates an exogenous mechanism of the formation of about a 4-day rhythm of the proliferative activity of cell culture.

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“…Infradian about 4-day fluctuations were found in many biological parameters: concentrations of melatonin, glucocorticoid and sex hormones 1 – 6 , motor activity 7 , immune system 8 , 9 , mitotic activity of cells 10 , 11 . Based on the facts that (1) 4-day rhythms are preserved during the removal both of the testes and adrenal glands 12 , and (2) hibernating hedgehogs with reduced hormonal activity had the stable rhythms of spontaneous short-term arousals 13 , it can be argued that the formation of this infradian rhythm is not associated with hormonal regulation.…”

Section: Introductionmentioning

confidence: 99%

About 4-day rhythm of proliferative activity of fibroblast-like cell cultures isn’t endogenous and don’t depend from the variations of Earth’s magnetic field

Диатроптова

Косырева

Diatroptov

2022

Sci Rep

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A study of the 4-day rhythm of the proliferative activity of the embryonic fibroblast-like cells in the logarithmic growth phase was carried out. It was shown that in cell cultures obtained on different days from embryos of different ages, the phase of the 4-day rhythm coincides. In vitro the maxima of the proliferative activity were consistent with the minima of the motor activity of mice. Freezing the culture for 2 or 6 days does not cause a shift in the phase of the 4-day rhythm of cell proliferative activity compare with the unfreezing culture. That indicates the existence of an external synchronizer, which determines the 4-day infradian rhythm of the proliferative activity of embryonic cells. Then we daily thawed samples of single L929 culture of mice fibroblast-like cells for 22 and 17 days and researched the dynamics of its proliferative activity. We also showed 4-day rhythm of the simultaneous increase in the number of cells for all thawed samples. Taking into account that deep freezing of a culture leads to the cessation of all life processes, the fact we obtained indicates an exogenous mechanism of the formation of about a 4-day rhythm of the proliferative activity of cell culture. Variations of the Earth's magnetic field could be one of the external synchronizers of the infradian rhythm. We studied the increase in number of L929 cell in conditions of a magnetic permalloy screen and showed that the magnetic shielding no affect the parameters of the infradian rhythm of L929 cell proliferative activity. So further searches of the external synchronizers are need.

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Characteristics of 4-Day Infradian Biorhythms in Mature Male Wistar Rats after Pinealectomy

Cited by 9 publications

References 5 publications

Melatonin-Loaded Nanoparticles for Enhanced Antidepressant Effects and HPA Hormone Modulation

Melatonin-Loaded Nanoparticles for Enhanced Antidepressant Effects and HPA Hormone Modulation

About 4-day Rhythm of Proliferative Activity of Fibroblast-like Cell Cultures Depends on the Environmental Factor

About 4-day rhythm of proliferative activity of fibroblast-like cell cultures isn’t endogenous and don’t depend from the variations of Earth’s magnetic field

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