Characteristics of a self-assembled fibrillar gel prepared from red stingray collagen

Abstract

A translucent collagen gel was formed from a transparent acidic solution of red stingray collagen by adjusting to physiological ionic strength and pH in phosphate buffer and then incubating at 25-37°C. During fibril formation from red stingray collagen, the turbidity increased when the NaCl concentration was increased at constant pH and the rate of fibril formation was accelerated by higher pH or lower NaCl concentration. The T m of red stingray collagen fibrillar gel was estimated as 44.3 ± 3.5°C, which was higher than that of the collagen solution, 33.2°C. In addition, red stingray collagen gel maintained its shape without melting and was suitable for culture of mouse stromal cells at 37°C.

Effects of phosphate ion concentration on in-vitro fibrillogenesis of sturgeon type I collagen

“…This inference is true for both SBC and SC. There have been a few studies on the fibrillogenesis of type I collagen and the morphology of the fibrils formed in vitro in fish collagens, such as those from salmon skin [26], shark skin [31], red stingray skin [32], Bester sturgeon skin, and swim bladder [27]. However, these studies focused on the influence of monovalent ions only, such as Na + and Cl -.…”

Effect of heat treatment on the enzymatic stability of grass carp skin collagen and its ability to form fibrils in vitro

“…The rate of fibril formation was monitored by measuring the turbidity changes as observed by the increase in absorbance at 310 nm using a UV spectrophotometer (UV‐2000, Unico, Shanghai, China). The degree of fibril formation was determined by the method of Bae et al Briefly, a collagen fibrillar gel was prepared in a centrifugation tube by the procedure described above. After 24 h of incubation, the gel was centrifuged at 10 000 × g for 10 min at 4 °C to precipitate the collagen fibrils, which were collected for analysis of fibril morphology.…”

Comparative study on characteristics and in vitro fibril formation ability of acid and pepsin soluble collagen from the skin of catla ( Catla catla ) and rohu ( Labeo rohita )

“…The degree of rohu skin collagen fibril formation significantly (p≤0.05) higher (up to 78% at 1500 mM NaCl concentration) as compared with catla skin collagen (36%). Bae et al (2009) andZhang et al (2014) have reported an increase in the degree of fibril formation with increasing NaCl concentration.…”