Characteristics of an established goldfish Carassim auratus (L.) cell line

Abstract

A new continuous line of goldfish somatic cells, designated SJU-1 has been continuously subcultured over a 39-month period. Best growth was obtained at 20" C over a pH range of 6.8-7.2. The minimal and optimal seed inocula, in terms of per cent cell increase, were determined to be 1.1 x 10' and 2.5 x lo8, respectively. The susceptibility of the SJU-1 line to infectious pancreatic necrosis virus offers an available assay system for goldfish in vivo and goldfish cells in vitro interferon studies. Chromosomal analyses of the line were also carried out.

Suppression of interferon synthesis by the pesticide carbaryl as a mechanism for enhancement of goldfish virus-2 replication

“…Synthesis of interferon by CAR cells in response to GFV-2 is not unexpected. Interferon synthesis in response to several viruses has been reported both in vivo and in vitro in a number of piscine systems, including the goldfish-derived cell line SJU-1 (8). Piscine interferon has been shown to be effective against another Iridovirus, frog virus type 3 (7).…”