Characteristics of glycoprotein B of equine herpesvirus 1 expressed by a recombinant baculovirus

Identification of equine herpesvirus-1 antigens recognized by cytotoxic T lymphocytes

“…The genes encoding the gC, gD, gE, gH, gI and gL glycoproteins, the IE protein and the UL5 protein were cloned into the PCR3.1 plasmid (Invitrogen). The gB glycoprotein was cloned into the pRCCMV plasmid (Invitrogen) (Munro et al, 1999). All plasmids were purified for PowderJect-XR1 particle mediated DNA transfection by anion exchange chromatography (Qiagen) and coated on to gold beads at a concentration of 2?5 mg DNA (mg gold beads) 21 , as previously described (Lunn et al, 1999).…”

Protective Effects of Intranasal Immunization with Recombinant Glycoprotein D in Pregnant BALB/c Mice Challenged with Different Strains of Equine Herpesvirus 1

“…Envelope glycoproteins play an important role in the infectivity and pathogenicity of EHV-1 and constitute major targets for the host immune system . These glycoproteins have been incorporated into recombinant vaccines (Love et al, 1993;Osterrieder et al, 1994;Munro et al, 1999). Experimental studies using individual or combined glycoproteins B, C, D and H have been performed in mice (Guo et al, 1990;Tewari et al, 1994Tewari et al, , 1995Osterrieder et al, 1995;Kukreja et al, 1998;Zhang et al, 1998;Ruitenberg et al, 1999) and in the natural host (Foote et al, 2005(Foote et al, , 2006.…”