Characteristics of <i>Ralstonia solanacearum</i> Biovar N2 Strains in Asia

Horita, Mitsuo; Tsuchiya, Kenichi; Ooshiro, Atsushi

doi:10.1111/j.1439-0434.2005.00954.x

Cited by 25 publications

(19 citation statements)

References 20 publications

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“…Thus, biovar II strains may be adapted to climates with relatively cooler temperatures than strains of biovars I and III, which grew well at higher temperatures. This is in agreement with previous reports that biovar II strains are adapted to cool tropical climates and biovars I and III strains are adapted to warm tropical climates (Horita et al 2005, Swanson et al 2005, Oepp/Eppo 2004, French et al 1993, Marin and ElNashaar 1993.…”

Section: Solanum Tuberosumsupporting

confidence: 93%

Isolation and characterisation of Ralstonia solanacearum strains from Solanaceae crops in Ethiopia

Lemessa

Zeller²

2007

J. Basic Microbiol.

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Eighty one isolates of Ralstonia solanacearum -like bacteria on triphenyl tetrazolium chloride (TTC) medium were collected from different Solanaceae crops (i.e. potato, tomato and pepper plants and potato tubers) at various sites in Ethiopia. Of these, 62 strains were identified as R. solanacearum based on their cultural characteristics on TTC medium, tomato pathogenicity bioassay, carbon source utilisation patterns and a specific PCR-based assay. By Hayward's classification method, based on carbon source utilisation, 19 of the 62 R. solanacearum strains were identified as biovar I and 43 strains were identified as biovar II. The biovar I strains exhibited a high growth rate at high temperatures (37 degrees C). Whereas the growth rate of biovar II strains was greatest at lower temperatures (22 degrees C). Biovar I strains had broader host range than biovar II strains, which were limited to potato, tomato, and eggplant. To our knowledge, this is the first report of R. solanacearum biovar I in Ethiopia. The existence of biovar I strains in Ethiopia raises concerns because they have a broader host range than biovar II strains.

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Section: Solanum Tuberosumsupporting

confidence: 93%

Isolation and characterisation of Ralstonia solanacearum strains from Solanaceae crops in Ethiopia

Lemessa

Zeller²

2007

J. Basic Microbiol.

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“…The bacterial strains used in this study are listed in Table 1. Additionally, published phenotypic data for 21 strains of R. solanacearum belonging to phylotypes I, II, III and IV (Hayward, 1964;He et al, 1983;Horita & Tsuchiya, 1999;Horita et al, 2005;Prior & Steva, 1990;Roberts et al, 1990) were included and compared with the results obtained in our study (Table 2). Phylogenetic analyses of 16S-23S rRNA ITS gene, ITS and egl sequences included data for strains with published genome sequences as well as sequences deposited in GenBank as part of previous studies (Tables S1, S2 and S5).…”

Section: Methodsmentioning

confidence: 99%

“…Tolerance of NaCl was observed on CPG (for R. solanacearum and BDB strains) and CA agar medium (for R. syzygii strains) containing 0, 3 or 5 % NaCl. Additionally, phenotypic data for 21 strains of R. solanacearum representing phylotypes I, II, III and IV (Hayward, 1964;He et al, 1983;Horita & Tsuchiya, 1999;Horita et al, 2005;Prior & Steva, 1990;Roberts et al, 1990) were included in the comparisons carried out in this study (Table 2). …”

Section: Methodsmentioning

confidence: 99%

“…Using a selected range of phenotypic tests based on data from this study (Tables S6-S8) and from six published papers (Hayward, 1964;He et al, 1983;Horita & Tsuchiya, 1999;Horita et al, 2005;Prior & Steva, 1990;Roberts et al, 1990) (Table S9), however, some phenotypic features were identified that are useful in distinguishing the different taxa within the R. solanacearum species complex (Table 4). Strains of phylotypes I and III can be differentiated from all phylotype II strains and the strains of phylotype IV, R. syzygii and BDB by their ability to utilize trehalose (Table 4).…”

Section: Ralstonia Syzygii Subsp Celebesensis Uqrs 627 T (Kc757025)mentioning

confidence: 99%

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Polyphasic taxonomic revision of the Ralstonia solanacearum species complex: proposal to emend the descriptions of Ralstonia solanacearum and Ralstonia syzygii and reclassify current R. syzygii strains as Ralstonia syzygii subsp. syzygii subsp. nov., R. solanacearum phylotype IV strains as Ralstonia syzygii subsp. indonesiensis subsp. nov., banana blood disease bacterium strains as Ralstonia syzygii subsp. celebesensis subsp. nov. and R. solanacearum phylotype I and III strains as Ralstonia pse

Safni

Cleenwerck

Vos

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

405

261

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The Ralstonia solanacearum species complex has long been recognized as a group of phenotypically diverse strains that can be subdivided into four phylotypes. Using a polyphasic taxonomic approach on an extensive set of strains, this study provides evidence for a taxonomic and nomenclatural revision of members of this complex. Data obtained from phylogenetic analysis of 16S-23S rRNA ITS gene sequences, 16S–23S rRNA intergenic spacer (ITS) region sequences and partial endoglucanase (egl) gene sequences and DNA–DNA hybridizations demonstrate that the R. solanacearum species complex comprises three genospecies. One of these includes the type strain of Ralstonia solanacearum and consists of strains of R. solanacearum phylotype II only. The second genospecies includes the type strain of Ralstonia syzygii and contains only phylotype IV strains. This genospecies is subdivided into three distinct groups, namely R. syzygii , the causal agent of Sumatra disease on clove trees in Indonesia, R. solanacearum phylotype IV strains isolated from different host plants mostly from Indonesia, and strains of the blood disease bacterium (BDB), the causal agent of the banana blood disease, a bacterial wilt disease in Indonesia that affects bananas and plantains. The last genospecies is composed of R. solanacearum strains that belong to phylotypes I and III. As these genospecies are also supported by phenotypic data that allow the differentiation of the three genospecies, the following taxonomic proposals are made: emendation of the descriptions of Ralstonia solanacearum and Ralstonia syzygii and descriptions of Ralstonia syzygii subsp. nov. (type strain R 001T = LMG 10661T = DSM 7385T) for the current R. syzygii strains, Ralstonia syzygii subsp. indonesiensis subsp. nov. (type strain UQRS 464T = LMG 27703T = DSM 27478T) for the current R. solanacearum phylotype IV strains, Ralstonia syzygii subsp. celebesensis subsp. nov. (type strain UQRS 627T = LMG 27706T = DSM 27477T) for the BDB strains and Ralstonia pseudosolanacearum sp. nov. (type strain UQRS 461T = LMG 9673T = NCPPB 1029T) for the strains of R. solanacearum phylotypes I and III.

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“…Vários métodos baseados na análise genômica têm sido empregados para avaliação da diversidade genética em R. solanacearum, entre os quais destacam-se: RAPD (Random Amplified Polymorphic DNA); análise do DNA ribossomal e do gene hrp; RC-PFGE (rare-cutting restriction endonucleases Pulsed-field gel electrophoresis); AFLP (Amplified Fragment Length Polymorphism) e rep-PCR (repetitive-PCR) (Smith et al, 1995;Jaunet & Wang, 1999;Poussier et al, 1999;Martins, 2000;Yu et al, 2003;Horita & Tsuchiya, 2001;Horita et al, 2005). Recentemente, Fegan & Prior (2005) propuseram um novo sistema de filotipagem para caracterizar R. solanacearum em nível subespecífico, mas que ainda aguarda aceitação por parte da comunidade científica.…”

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Diversidade patogênica e molecular de Ralstonia solanacearum da região amazônica brasileira

2007

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INTRODUÇÃORalstonia solanacearum (Smith, 1896) Yabuuchi et al.1995 é o agente causador da murcha bacteriana em mais de 50 espécies botânicas (Hayward, 1994). Está distribuída principalmente em regiões de clima tropical e subtropical, onde prevalecem condições de alta umidade e elevadas temperaturas (Hayward, 1991). Suas complexidades genotípica e fenotípica, e ampla distribuição no mundo levantaram questionamentos em relação à sua evolução em diferentes áreas geográficas, inclusive na Amazônia brasileira (Buddenhagen, 1985). Relatos indicam que é parte da flora microbiana nativa em muitas partes do mundo e a ocorrência de epidemias é conseqüência da introdução RESUMOFoi avaliada a diversidade de isolados de Ralstonia solanacearum obtidos de tomateiro e de outras hospedeiras com sintomas de murcha bacteriana na região amazônica. Os isolados foram identificados quanto à biovar e separados em graus de virulência em plantas de tomate, pimentão e chicória da Amazônia (Eryngium foetidum). Dos 70 isolados, 53 pertenciam à biovar 1, quatro à biovar N2 e 13 à biovar 3, confirmando a predominância da biovar 1 em tomateiro no Estado do Amazonas. O agrupamento dos isolados mostrou três classes distintas de virulência em tomate, sendo 44,3% dos isolados altamente virulentos, 37,1% medianamente virulentos e 18,6% fracamente virulentos. O agrupamento em pimentão classificou 20% de isolados como altamente virulentos, 27,1% como medianamente virulentos e 52,9% como fracamente virulentos. Quando inoculados em chicória da Amazônia, somente o isolado de chicória provocou murcha nesta hospedeira, sugerindo uma especificidade pouco comum para R. solanacearum. Na caracterização molecular, 46 isolados de tomateiro e 18 de outras 10 hospedeiras, coletados em áreas de terra-firme e de várzea, foram comparados por BOX-PCR. Os perfis genômicos revelaram alto grau de polimorfismo entre os isolados, divididos em cinco grupos, sem correlação entre hospedeira de origem, biovar, ecossistema ou local de coleta. O isolado de chicória da Amazônia foi o mais divergente, com apenas 6,4% de similaridade em relação aos demais. Os isolados de tomateiro estavam representados em três grupos. Os quatro isolados de tomateiro da biovar N2 formaram um agrupamento distinto dos isolados das demais biovares presentes na Amazônia.Palavras-chave adicionais: murcha bacteriana, Lycopersicon esculentum, seqüências repetitivas, rep-PCR. ABSTRACT Pathogenic and molecular diversity of Ralstonia solanacearum isolates from the Brazilian AmazonThe diversity among 70 isolates of Ralstonia solanacearum collected from tomato and other hosts in the Brazilian Amazon was evaluated. Firstly, the isolates were identified at the biovar level and their virulence assessed by inoculating seedlings of tomato, sweet pepper and Amazon chicory (Eryngium foetidum). Fifty-three isolates were identified as biovar 1, four as biovar N2 and 13 as biovar 3, therefore confirming the prevalence of biovar 1 associated with tomato in the North Region of Brazil. Cluster analysis of the isolates...

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Characteristics of Ralstonia solanacearum Biovar N2 Strains in Asia

Cited by 25 publications

References 20 publications

Isolation and characterisation of Ralstonia solanacearum strains from Solanaceae crops in Ethiopia

Isolation and characterisation of Ralstonia solanacearum strains from Solanaceae crops in Ethiopia

Diversidade patogênica e molecular de Ralstonia solanacearum da região amazônica brasileira

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