Characteristics of Ice-nucleation Activity in<i>Fusarium avenaceum</i>IFO 7158

Hasegawa, Yoshie; Ishihara, Yoshimasa; Tokuyama, Tai

doi:10.1271/bbb.58.2273

Cited by 38 publications

(39 citation statements)

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“…In the organic grass/alfalfa and sugar beet soils it reduced INPs active ≥ −6 • C by ≈ 10-fold (Fig. 9), as would be expected for deactivation of IN Fusarium (Hasegawa et al, 1994;Richard et al, 1996;Pouleur et al, 1992;Humphreys et al, 2001) and Mortierella, but it had no effect on the roadside pasture soil. The cultivated soils thus contained some Proteinase Ksensitive IN microflora.…”

Section: In Fungimentioning

confidence: 55%

“…These species contain strains that are plant pathogens as well as saprophytes and are promoted by agricultural practices. Digestion of cultures of F. avenaceum and F. acuminatum with Proteinase K significantly reduced ice nucleation activity, suggesting that a protein is responsible (Hasegawa et al, 1994;Humphreys et al, 2001). In the agricultural soils tested, incubation with Proteinase K had a minimal affect upon overall INP concentrations.…”

Section: In Fungimentioning

confidence: 94%

“…This includes leaf-derived ice nuclei (Schnell and Vali, 1973), most strains of IN M. alpina , IN lichen (Kieft and Ruscetti, 1990), and IN Fusarium spp. (Pouleur et al, 1992), although the onset temperature of activity of cell-free INPs of F. avenaceum was lowered when heated > 40 • C (Hasegawa et al, 1994). It should also be noted that immobilization of proteins onto clays or tannins can significantly affect their sensitivity (either increase or decrease) to denaturation by 60 • C heat (Rao et al, 2000).…”

Section: Heat Treatmentsmentioning

confidence: 98%

“…To test soils for the presence of INPs susceptible to digestion by Proteinase K, such as those possessed by IN Fusarium species (Hasegawa et al, 1994;Humphreys et al, 2001), suspensions of the agricultural soils were digested with 5 U mL −1 Proteinase K (Qiagen) using the same buffer, incubation conditions and negative controls as used for lysozyme (also optimal for this enzyme). Soil suspensions (1 in 20 dilution) were also digested with papain protease (AppliChem) at concentrations of 10 or 20 mg mL −1 in 10 mM sodium phosphate buffer (pH 6.3) at 60 • C for 20 min.…”

Section: Enzymatic Digestionsmentioning

confidence: 99%

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Sources of organic ice nucleating particles in soils

et al. 2016

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Abstract. Soil organic matter (SOM) may be a significant source of atmospheric ice nucleating particles (INPs), especially of those active > −15 • C. However, due to both a lack of investigations and the complexity of the SOM itself, the identities of these INPs remain unknown. To more comprehensively characterize organic INPs we tested locally representative soils in Wyoming and Colorado for total organic By contrast, lysozyme, which digests bacterial cell walls, only reduced INPs active at ≥ −7.5 or ≥ −6 • C, depending on the soil. The known IN bacteria were not detected in any soil, using PCR for the ina gene that codes for the active protein. We directly isolated and photographed two INPs from soil, using repeated cycles of freeze testing and subdivision of droplets of dilute soil suspensions; they were complex and apparently organic entities. Ice nucleation activity was not affected by digestion of Proteinase K-susceptible proteins or the removal of entities composed of fulvic and humic acids, sterols, or aliphatic alcohol monolayers. Organic INPs active colder than −10 to −12 • C were resistant to all investigations other than heat, oxidation with H 2 O 2 , and, for some, digestion with papain. They may originate from decomposing plant material, microbial biomass, and/or the humin component of the SOM. In the case of the latter then they are most likely to be a carbohydrate. Reflecting the diversity of the SOM itself, soil INPs have a range of sources which occur with differing relative abundances.

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Section: In Fungimentioning

confidence: 55%

Section: In Fungimentioning

confidence: 94%

Section: Heat Treatmentsmentioning

confidence: 98%

Section: Enzymatic Digestionsmentioning

confidence: 99%

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Sources of organic ice nucleating particles in soils

et al. 2016

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“…In the past, several researchers studied fungal ice nucleation and found high potential ice nuclei in some lichen mycobionts (Kieft, 1988;Kieft and Ahmadjian, 1989;Kieft and Ruscetti, 1990) and some species of the plant pathogenic fungal genus Fusarium (Pouleur et al, 1992;Hasegawa et al, 1994;Tsumuki and Konno, 1994;Tsumuki et al, 1995). It was proposed that these ice nuclei are proteins, but have 8084 B. G. Pummer et al: Spores of many common airborne fungi reveal no ice nucleation activity little in common with the well-known bacterial ice nucleation proteins (Kawahara, 2002).…”

Section: Introductionmentioning

confidence: 99%

Spores of many common airborne fungi reveal no ice nucleation activity in oil immersion freezing experiments

et al. 2013

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Abstract. Fungal spores are ubiquitous biological aerosols, which are considered to act as ice nuclei. In this study the ice nucleation (IN) activity of spores harvested from 29 fungal strains belonging to 21 different species was tested in the immersion freezing mode by microscopic observation of waterin-oil emulsions. Spores of 8 of these strains were also investigated in a microdroplet freezing array instrument. The focus was laid on species of economical, ecological or sanitary significance. Besides common molds (Ascomycota), some representatives of the widespread group of mushrooms (Basidiomycota) were also investigated.Fusarium avenaceum was the only sample showing IN activity at relatively high temperatures (about 264 K), while the other investigated fungal spores showed no freezing above 248 K. Many of the samples indeed froze at homogeneous ice nucleation temperatures (about 237 K). In combination with other studies, this suggests that only a limited number of species may act as atmospheric ice nuclei. This would be analogous to what is already known for the bacterial ice nuclei.Apart from that, we selected a set of fungal strains from different sites and exposed them to occasional freezing stress during their cultivation. This was in order to test if the exposure to a cold environment encourages the expression of ice nuclei during growth as a way of adaptation. Although the total protein expression was altered by this treatment, it had no significant impact on the IN activity.

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Applications of biological ice nucleators

Lundheim¹,

Zachariassen

1999

Biotechnological Applications of Cold-Adapted Organisms

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Characteristics of Ice-nucleation Activity inFusarium avenaceumIFO 7158

Cited by 38 publications

References 1 publication

Sources of organic ice nucleating particles in soils

Sources of organic ice nucleating particles in soils

Spores of many common airborne fungi reveal no ice nucleation activity in oil immersion freezing experiments

Applications of biological ice nucleators

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