Characteristics of L-lactic Acid Production by<i>Rhizopus oryzae</i>mutant RL6041

Zhou, Xiuhong; Ge, Cui; Yao, Jianming; Renrui, Pan; Zhang, Yu

doi:10.1080/08905430500316409

Cited by 3 publications

(3 citation statements)

References 6 publications

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“…As can be seen from Figure 2, 3 , every time changed the load in influent, the reactor will be affected, the NH 4 + -N and NO 2 -N were volatile in effluent, indicating that the reactor shock load is poor, may be due anammox bacteria is not abundant in reactor. [4] In the mid-late experiment, the removal rate of NH 4 + -N and NO 2 --N generally over 50%, and with the removal rate of NH 4 + -N increased , the removal rate of NO 2 --N was also increasing. In the late experiment , the NO 2 --N and NH 4 + -N ratio in the effluent basically maintained at about 1, it can be determined that the anammox bacteria in reactor have become the dominant species [5] .…”

Section: Results Discussionmentioning

confidence: 92%

Start-Up of Anammox Process Using Municipal Sewage Sludge in One UASB Reactor

Hua

Zhang

Hou

et al. 2014

AMR

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Using municipal sewage treatment plant sludge as the inoculation sludge, anaerobic ammonia oxidation experiment started in a UASB reactor with an effective volume of 3.5L. The experiment lasted 116d, experimental medium term, the system NH4+-N, NO2--N removal rates were above 50%. Changing influent load will cause a greater impact on the reactor, resulting in fluctuations of NH4+-N and NO2--N removal rate. Late, NO2--N / NH4+-N values remain at around 1. The sludge in the reactor changed from gray-brown to pink. The system is more stable for removing NH4+-N and NO2--N.

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Section: Results Discussionmentioning

confidence: 92%

Start-Up of Anammox Process Using Municipal Sewage Sludge in One UASB Reactor

Hua

Zhang

Hou

et al. 2014

AMR

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“…The DMOG dose inhibiting TET is 10 times different with the dose to regulate HIF. 28,29 Also, many studies used DMOG as a TET inhibitor to inhibit TET activity both in vivo and in vitro. [30][31][32] Based on this presented the IOP change in response to rhGDF7 delivery or/and nGDF7 treatment in monkey eyes (n = 4 per group).…”

Section: Discussionmentioning

confidence: 99%

TET-dependent GDF7 hypomethylation impairs aqueous humor outflow and serves as a potential therapeutic target in glaucoma

et al. 2021

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Glaucoma is the leading cause of irreversible vision loss, affecting more than 70 million individuals worldwide. Circulatory disturbances of aqueous humor (AH) have long been central pathological contributors to glaucomatous lesions. Thus, targeting the AH outflow is a promising approach to treat glaucoma. However, the epigenetic mechanisms initiating AH outflow disorders and the targeted treatments remain to be developed. Studying glaucoma patients, we identified GDF7 (growth differentiation factor 7) hypomethylation as a crucial event in the onset of AH outflow disorders. Regarding the underlying mechanism, the hypomethylated GDF7 promoter was responsible for the increased GDF7 production and secretion in primary open-angle glaucoma (POAG). Excessive GDF7 protein promoted trabecular meshwork (TM) fibrosis through bone morphogenetic protein receptor type 2 (BMPR2)/Smad signaling and upregulated pro-fibrotic genes, a-smooth muscle actin (a-SMA) and fibronectin (FN). GDF7 protein expression formed a positive feedback loop in glaucomatous TM (GTM). This positive feedback loop was dependent on the activated TET (ten-eleven translocation) enzyme, which kept the GDF7 promoter region hypomethylated. The phenotypic transition in TM fortified the AH outflow resistance, thus elevating the intraocular pressure (IOP) and attenuating the nerve fiber layer. This methylation-dependent mechanism is also confirmed by a machine-learning model in silico with a specificity of 84.38% and a sensitivity of 89.38%. In rhesus monkeys, we developed GDF7 neutralization therapy to inhibit TM fibrosis and consequent AH outflow resistance that contributes to glaucoma. The neutralization therapy achieved high-efficiency control of the IOP (from 21.3 ± 0.3 to 17.6 ± 0.2 mmHg), a three-fold improvement in the outflow facility (from 0.1 to 0.3 mL/min $ mmHg), and protection of nerve fibers. This study provides new insights into the epigenetic mechanism of glaucoma and proposes an innovative GDF7 neutralization therapy as a promising intervention.

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“…This study presents the potential of Rhizopus oryzae fermentation in a bioreactor on various concentrations of pearled oat flour for the production of lactic acid. In most previous studies, the carbon source that was used in fungal fermentations for lactic acid production was either glucose ( , ) or liquefied/saccharified starch solutions ( 5 , − . The nitrogen sources used in those studies were mainly inorganic chemicals or byproducts of food industries.…”

Section: Introductionmentioning

confidence: 99%

Development of an Oat-Based Biorefinery for the Production of l(+)-Lactic Acid by Rhizopus oryzae and Various Value-Added Coproducts

Koutinas

Malbranque

Wang

et al. 2007

J. Agric. Food Chem.

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A novel oat-based biorefinery producing L(+)-lactic acid and various value-added coproducts (e.g., beta-glucan, anti-irritant solution) is proposed. Pearling is employed for sequential separation of bran-rich fractions for the extraction of value-added coproducts. Lactic acid production is achieved via fungal fermentation of Rhizopus oryzae on pearled oat flour. Maximum lactic acid concentration (51.7 g/L) and starch conversion yield (0.68 g/g) were achieved when an oat flour concentration of 116.5 g/L was used. Oxygen transfer played a significant role with respect to lactic acid production and starch conversion yield. Rhizopus oryzae produced a range of enzymes (glucoamylase, protease, phosphatase) for the hydrolysis of cereal flour macromolecules. Enzyme production during fungal fermentation has been reported. The proposed biorefining strategy could lead to significant operating cost reduction as compared to current industrial practices for lactic acid production from pure glucose achieved by bacterial fermentations.

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Characteristics of L-lactic Acid Production byRhizopus oryzaemutant RL6041

Cited by 3 publications

References 6 publications

Start-Up of Anammox Process Using Municipal Sewage Sludge in One UASB Reactor

Start-Up of Anammox Process Using Municipal Sewage Sludge in One UASB Reactor

TET-dependent GDF7 hypomethylation impairs aqueous humor outflow and serves as a potential therapeutic target in glaucoma

Development of an Oat-Based Biorefinery for the Production of l(+)-Lactic Acid by Rhizopus oryzae and Various Value-Added Coproducts

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