Characteristics of Microfluidic Paper-based Analytical Devices Fabricated by Four Different Methods

Komatsu, Takeshi; Maeki, Masatoshi; Ishida, Akihiko; Tani, Hirofumi

doi:10.2116/analsci.34.39

Cited by 18 publications

(14 citation statements)

References 30 publications

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“…5,6 Various detection methods such as chemiluminescence, fluorescence, electrochemical and colorimetric detection have been applied to μPADs for rapid, sensitive and selective analysis. 7,8 Among these detection methods, colorimetric detection has emerged as a useful tool for rapid qualitative analysis due to its ability to be used in remote locations or those with poor infrastructure where sophisticated tools or timeconsuming steps cannot be afforded. 9 Moreover, the presence of the analyte in the detection zone causes a color change, enabling visual readout, which does not require special equipment for analysis and makes the system cheaper.…”

Section: Introductionmentioning

confidence: 99%

A Portable Smartphone-based Platform with an Offline Image-processing Tool for the Rapid Paper-based Colorimetric Detection of Glucose in Artificial Saliva

2020

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In this study, a microfluidic paper-based analytical device (μPAD) was integrated with a smartphone app capable of offline (without internet access) image processing and analysis for the rapid colorimetric detection of glucose. A selfinking stamp was used to form hydrophobic channels on a piece of paper-towel due to its superior water absorption efficiency. As demonstrated, the developed sensor was employed for the colorimetric detection of glucose in artificial saliva in the linear scope of 0 -1 mM with a calculated detection limit of 29.65 μM. The experimental results show that the quantitative analysis of glucose with the proposed smartphone platform could be completed in less than one minute. The app developed for the smartphone platform is capable of extracting the color-changing area with an embedded image processing tool which could address the problem of color uniformity in the detection zones of μPAD. The integrated platform has great potential to be used for non-invasive measurements of glucose in body fluids, like tears, sweat and saliva.

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Section: Introductionmentioning

confidence: 99%

A Portable Smartphone-based Platform with an Offline Image-processing Tool for the Rapid Paper-based Colorimetric Detection of Glucose in Artificial Saliva

2020

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“…Multiple processes can be applied to create a hydrophobic barrier on paper, including photolithography [15,16], light irradiation [17], plasma treatment [18], laser cutting [19], stamping [20], inkjet printing [21,22], wax printing [23,24], and screen printing [25,26]. However, photolithography, plasma-treatment, laser-cutting, and inkjet-printing techniques have some limitations, such as expensive fabrication instrumentation, multi-step fabrication processes, and long fabrication times [27]. Wax printing enables easy fabrication and low cost but requires a heating step that causes the wax to spread, decreasing the feature resolution [27,28].…”

Section: Introductionmentioning

confidence: 99%

“…However, photolithography, plasma-treatment, laser-cutting, and inkjet-printing techniques have some limitations, such as expensive fabrication instrumentation, multi-step fabrication processes, and long fabrication times [27]. Wax printing enables easy fabrication and low cost but requires a heating step that causes the wax to spread, decreasing the feature resolution [27,28]. Alternative materials such as polymer solutions, polystyrene [28], polydimethylsiloxane [29], and polycaprolactone [30] have been used to create hydrophobic regions on µPADs to overcome wax material's drawbacks.…”

Section: Introductionmentioning

confidence: 99%

One-Step Polylactic Acid Screen-Printing Microfluidic Paper-Based Analytical Device: Application for Simultaneous Detection of Nitrite and Nitrate in Food Samples

2019

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In this work, we report a one-step approach for fabricating screened-printed microfluidic paper-based analytical devices (μPADs) using polylactic acid as a new hydrophobic material. A polylactic acid solution was screen printed onto chromatography papers to create hydrophobic patterns for fluidic channels. The optimal polylactic acid concentration for successful device fabrication is 9% w/v. The μPADs were fabricated within 2 min and provided high reproducibility and stability. The utility of polylactic acid screen-printing was demonstrated for the simultaneous detection of nitrite and nitrate using colorimetric detection. Under optimized experimental conditions, the detection limits and the linear ranges, respectively, were 1.2 mg L−1 and 2–10 mg L−1 for nitrite and 3.6 mg L−1 and 10–50 mg L−1 for nitrate. The detection times for both ions were found to be within 12 min. The developed μPAD was applied for the simultaneous determination of these ions in food samples, and no significant differences in the analytical results were observed compared to those of the reference method. The polylactic acid screen-printing approach presented here provides a simple, rapid, and cost-effective alternative fabrication method for fabricating μPADs.

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“…Different technologies are being used or under development for POC diagnostic assays, which are easy to use, highly sensitive for low-limit detection and cost effective. 9 Nanotechnology, a most innovative and attractive technology, has the potential to be used for portable POC applications. Currently, researchers are working to incorporate nanomaterials in POC diagnostic assays to introduce new innovations to develop diagnostic assays that offer the advantages of low cost, greater sensitivity, requirement for fewer reagents, and being contamination free.…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle-based Point of Care Immunoassays for in vitro Biomedical Diagnostics

2018

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In resource-limited settings, the availability of medical practitioners and early diagnostic facilities are inadequate relative to the population size and disease burden. To address cost and delayed time issues in diagnostics, strip-based immunoassays, e.g. dipstick, lateral flow assay (LFA) and microfluidic paper-based analytical devices (microPADs), have emerged as promising alternatives to conventional diagnostic approaches. These assays rely on chromogenic agents to detect disease biomarkers. However, limited specificity and sensitivity have motivated scientists to improve the efficiency of these assays by conjugating chromogenic agents with nanoparticles for enhanced qualitative and quantitative output. Various nanomaterials, which include metallic, magnetic and luminescent nanoparticles, are being used in the fabrication of biosensors to detect and quantify biomolecules and disease biomarkers. This review discusses some of the principles and applications of such nanoparticle-based point of care biosensors in biomedical diagnosis.

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Characteristics of Microfluidic Paper-based Analytical Devices Fabricated by Four Different Methods

Cited by 18 publications

References 30 publications

A Portable Smartphone-based Platform with an Offline Image-processing Tool for the Rapid Paper-based Colorimetric Detection of Glucose in Artificial Saliva

A Portable Smartphone-based Platform with an Offline Image-processing Tool for the Rapid Paper-based Colorimetric Detection of Glucose in Artificial Saliva

One-Step Polylactic Acid Screen-Printing Microfluidic Paper-Based Analytical Device: Application for Simultaneous Detection of Nitrite and Nitrate in Food Samples

Nanoparticle-based Point of Care Immunoassays for in vitro Biomedical Diagnostics

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