Characteristics of nonneoplastic human prostate tissue transplanted into nude mice

Evidence of pluripotent human prostate stem cells in a human prostate primary xenograft model

“…This short-term (1-2 months) human prostate primary xenograft model retains the human prostate architecture for both benign and CaP specimens and demonstrates that the progenitor stem cells respond to transplantation, androgen deprivation, and environmental signaling. Minor histological differences were observed in the human prostate xenografts, compared to the initial tissue, including inflammation, squamous metaplasia, and basal cell hyperplasia [18,19]. However, the expression of the secretory cell markers AR and PSA, basal cell marker HMW-CK, and apoptotic and proliferation indices were recapitulated in the 1-month xenograft compared to the initial specimen [18].…”

“…To this end, our laboratory has investigated the use of human prostate primary xenografts maintained in immunocompromised hosts as a model for the role of prostate stem cells in human CaP [18][19][20]. This study focuses on the pluripotentiality demonstrated by prostate stem cells as they repopulate the epithelial compartment following transplantation, and after stimulation with testosterone for 2 days following 1 month of androgen deprivation induced by castration.…”

Immunohistochemical Detection of Carcinogen‐DNA Adducts in Normal Human Prostate Tissues Transplanted into the Subcutis of Athymic Nude Mice: Results with 2‐Amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]pyridine (PhIP) and 3,2′‐Dimethyl‐4‐aminobiphenyl (DMAB) and Relation to Cytochrome P450s and N‐Acetyltransferase Activity

“…16) Data on immunohistochemical localization of PhIP-or DMAB-DNA adducts in transplanted human prostate and mouse tissues are summarized in Tables I and II. Both epithelial and stromal cells in human prostate xenografts obtained from each individual demonstrated positive nuclear staining for anti-PhIP-DNA (Fig.…”

“…Prostate tissue preparation and transplantation into the nude mice were conducted as described elsewhere. 16) Briefly, prostate glands were separated from the bladder and placed in culture medium (Dulbecco's MEM with 12.5 mM HEPES, Gibco BRL, Rockville, MD), and macroscopically divided into peripheral and transition zones. Small portions were fixed in formalin for histological evaluation as 0-week samples, and the remaining tissues were cut into approximately 5-mm cubes.…”

“…15) To investigate the possible relationship of PhIP with human prostate carcinogenesis, we investigated whether PhIP-DNA adducts are formed in human prostatic cells in vivo using a normal human prostate xenograft model. 16) A preliminary finding on PhIP-DNA adduct formation in transplanted human prostate tissue has been reported. 17) An analysis of carcinogen-DNA adducts formed by the synthetic aromatic amine, 3,2′-dimethyl-4-aminobiphenyl (DMAB), which produces prostate tumors as well as colon, Zymbal gland and urinary bladder cancer in rodents was also presented.…”