Characteristics of oleate binding to liver plasma membranes and its uptake by isolated hepatocytes

Stump, Decherd; Nunes, Rosa; Sorrentino, Dario; Isola, Luis; Berk, Paul D.

doi:10.1016/s0168-8278(05)80661-0

Cited by 45 publications

(28 citation statements)

References 43 publications

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“…Initial rates of LCFA transport into isolated rat adipocytes are maximal and linear for 15 s, are a function of concentration of unbound LCFA in the medium, and demonstrate both saturable and nonsaturable components (3,66,67). The saturable components are observed primarily at LCFA-to-BSA (LCFA/ BSA) ratios Ͻ3.0, consistent with the physiological range.…”

Section: Kinetic Evidence For Protein-mediated Transport In Eukaryotisupporting

confidence: 55%

Fatty acid transport: the roads taken

Schaffer

2002

American Journal of Physiology-Endocrinology and Metabolism

151

112

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Efficient uptake and channeling of long-chain fatty acids (LCFAs) are critical cellular functions. Although spontaneous flip-flop of nonionized LCFAs from one leaflet of a bilayer to the other is rapid, evidence is emerging that proteins are important mediators and/or regulators of trafficking of LCFAs into and within cells. Genetic screens have led to the identification of proteins that are required for fatty acid import and utilization in prokaryotic organisms. In addition, functional screens have elucidated proteins that facilitate fatty acid import into mammalian cells. Although the mechanisms by which these proteins mediate LCFA import are not well understood, studies in both prokaryotic and eukaryotic organisms provide compelling evidence that uptake of LCFAs across cellular membranes is coupled to esterification by acyl-CoA synthetases. This review will summarize results of studies of non-protein-mediated and protein-mediated LCFA transport and discuss how these different mechanisms may contribute to cellular metabolism.

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Section: Kinetic Evidence For Protein-mediated Transport In Eukaryotisupporting

confidence: 55%

Fatty acid transport: the roads taken

Schaffer

2002

American Journal of Physiology-Endocrinology and Metabolism

151

112

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“…Fatty acid uptake is the sum of saturable and linear processes ( 14 ). Fatty acid uptake in the physiological range of unbound fatty acids is largely driven by the saturable process and, in the current study, failed to activate PPAR-␣ .…”

Section: Discussionmentioning

confidence: 48%

VLDL hydrolysis by LPL activates PPAR-α through generation of unbound fatty acids

Ruby¹,

Goldenson²,

Orasanu

et al. 2010

Journal of Lipid Research

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“…1,2,23 Correlations among measured variables V max was highly significantly correlated with both body weight (r ¼ 0.908) ( Figure 3) and BMI (r ¼ 0.869) (Po0.01 in each case). As expected, both plasma insulin and leptin concentrations were also correlated with BMI (r ¼ 0.669 and 0.635, respectively) and body weight (r ¼ 0.680 and 0.640, respectively) (Po0.01 in each instance).…”

Section: Lcfa Uptake Studiesmentioning

confidence: 86%

“…1,2 Based on determination of the t 1/2 s for the movement of a fatty acid molecule across the plasma membranes of isolated rat adipocytes and hepatocytes, the facilitated process is at least 10-fold faster than that of passive flip-flop. 2,23 Under basal physiologic conditions, more than 90% of LCFA uptake by hepatocytes, adipocytes, and cardiac myocytes occurs via the saturable, facilitated process. 9,11,13,27,28 Altered LCFA disposition is typical of both obesity and NIDDM.…”

Section: Discussionmentioning

confidence: 99%

Long-chain fatty acid uptake is upregulated in omental adipocytes from patients undergoing bariatric surgery for obesity

et al. 2004

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OBJECTIVE:To determine the impact of obesity on adipocyte cell size and long-chain fatty acid (LCFA) uptake kinetics in human subjects undergoing laparoscopic abdominal surgery. SUBJECTS: A total of 10 obese patients (BMI 49.8711.9 (s.d.) kg/m 2 ) undergoing laparoscopic bariatric surgery, and 10 nonobese subjects (BMI 24.272.3 kg/m 2 ) undergoing other clinically indicated laparoscopic abdominal surgical procedures. MEASUREMENTS: Cell size distribution and [ 3 H]oleic acid uptake kinetics were studied in adipocytes isolated from omental fat biopsies obtained during surgery. Adipocyte surface area (SA) was calculated from the measured cell diameters. Plasma leptin and insulin concentrations were measured by RIA in fasting blood samples obtained on the morning of surgery. RESULTS: The mean SA of obese adipocytes (41 50875381 m 2 /cell) was increased 2.4-fold compared to that of nonobese adipocytes (16 92876529 m 2 /cell; Po0.01). LCFA uptake in each group was the sum of saturable and nonsaturable components. Both the V max of the saturable component (21.376.3 vs 5.171.9 pmol/s/50 000 cells) and the rate constant k of the nonsaturable component (0.01570.002 vs 0.006670.0023 ml/s/50 000 cells) were increased (Po0.001) in obese adipocytes compared with nonobese controls. When expressed relative to cell size, V max /m 2 SA was greater in obese than nonobese adipocytes (Po0.05), whereas k/m 2 SA did not differ between the groups. CONCLUSION: The data support the concepts that (1) adipocyte LCFA uptake consists of distinct facilitated (saturable) and diffusive processes; (2) increased saturable LCFA uptake in obese adipocytes is not simply a consequence of increased cell size, but rather reflects upregulation of a facilitated transport process; and (3) the permeability of adipocyte plasma membranes to LCFA is not appreciably altered by obesity, and increased nonsaturable uptake in obese adipocytes principally reflects an increase in cell SA. Regulation of saturable LCFA uptake by adipocytes may be an important control point for body adiposity.

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Characteristics of oleate binding to liver plasma membranes and its uptake by isolated hepatocytes

Cited by 45 publications

References 43 publications

Fatty acid transport: the roads taken

Fatty acid transport: the roads taken

VLDL hydrolysis by LPL activates PPAR-α through generation of unbound fatty acids

Long-chain fatty acid uptake is upregulated in omental adipocytes from patients undergoing bariatric surgery for obesity

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