Characteristics of Persistent Salmonella Enteritidis Strains in Two Integrated Broiler Chicken Operations of Korea

Abstract

ABSTRACT. The objectives of the study were to investigate the phenotypic and genotypic characterization of the persistent Salmonella Enteritidis (S. Enteritidis) isolates in two integrated broiler chicken operations, with attention focused mainly on the epidemiological approach. In the distribution of virulence genes, Salmonella enterotoxin (stn), invading host cell (invA), and Salmonella plasmid virulence (spvC) genes were widely distributed among the S. Enteritidis irrespective of their source of isolation, and Salmonella fimbrial (sefC) and plasmid encoded fimbrial (pef) genes were present in 28 and 20 S. Enteritidis strains, respectively. A total of 5 different XbaI-PFGE types were obtained from 31 S. Enteritidis isolates. Twenty-one types were divided on the basis their PFGE pattern, phage type and antimicrobial resistance pattern determined. There was a significant difference in phenotypic and genotypic characterization by two integrated broiler operations. Also, 8 isolates shown susceptible to all antimicrobials and 11 isolates with resistance to nalidixic acid were partly classified by XbaI PFGE pattern and by the phage type.

Prevalence of Virulence Genes in Extended‐Spectrum β‐lactamases (ESBLs)‐Producing Salmonella in Retail Raw Chicken in China

“…The majorities (98.7%) of ESBLs‐producing Salmonella isolates in this study harbored at least one virulence gene, which highlights their potential pathogenesis and the risk associated with this pathogen in retail chickens in China. Compared to the results of other studies on Salmonella (Huehn et al., ; Kwag et al., ), the detection percentage of virulence genes among ESBLs‐producing Salmonella in this study were different compared to other studies. Although virulence genes, such as sip A, sod C1, sop E2, rck , spv C, msg A, and pef A, were commonly detected in some foodborne Salmonella strains linked to human salmonellosis cases, clinical diarrhea and infections, these genes were rarely detected in our isolates (Capuano et al., ; Huehn et al., ; Mezal et al., ; Osman, Marouf, & Alatfeehy, ; Zhang et al., ; Zou et al., ).…”

Molecular Characterization ofSalmonella entericaSerotype Enteritidis Isolates from Humans by Antimicrobial Resistance, Virulence Genes, and Pulsed-Field Gel Electrophoresis

“…The spvC and rck genes were found in 91.3% and 89.2% of strains, respectively. These findings are in agreement with previous studies (Kwag et al, 2008;Hur et al, 2011;Huehn et al, 2010;Bolton et al, 2011), which have also reported the high prevalence of spvC and rck genes in salmonella isolates. A self-transferable virulence-resistance plasmid (pUO-StVR2) carrying the spvA, spvB, spvC, and rck gene was earlier reported in S. Typhimurium (Guerra et al, 2002).…”

Prevalence and Characteristics of <i>Salmonella</i> spp. Isolated from Poultry Slaughterhouses in Korea

“…However, PT4 has not appeared in the literature since that time [11,28,29]. Recently, Kang et al [24] reported PT1 and PT21 accounted for 27.2% and 20.8%, respectively; however, PT4 has been found in only 8.7%.…”