Characteristics of Scopolamine-releasing Hairy Root Clones of<i>Duboisia leichhardtii</i>

Muranaka, Toshiya; Kazuoka, Toru; Ohkawa, Hideo; Yamada, Yasuyuki

doi:10.1271/bbb.57.1398

Cited by 10 publications

(2 citation statements)

References 8 publications

(12 reference statements)

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“…Different culture systems such as shake flasks, bioreactors, airlift reactors, and turbine blade reactors have been utilized for secondary metabolite production in hairy root cultures in different solanaceous plants like Atropa belladonna, Duboisia leichhardtii, and Physalis minima (Muranaka et al, 1992;Muranaka et al, 1993a;Muranaka et al, 1993b;Gansau and Mahmood, 2013;Habibi et al, 2015) (Supplementary Table 1). The hairy roots of Anisodus tanguiticus accumulated greater biomass, when the culture volume of flask reactors was increased (Lei et al, 2020).…”

Section: Culture Systems Used For Scaling Up Of Hairy Root Productionmentioning

confidence: 99%

Hairy root culture: a potent method for improved secondary metabolite production of Solanaceous plants

Biswas,

Chakraborty,

Mukherjee

et al. 2023

Front. Plant Sci.

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Secondary metabolites synthesized by the Solanaceous plants are of major therapeutic and pharmaceutical importance, many of which are commonly obtained from the roots of these plants. ‘Hairy roots’, mirroring the same phytochemical pattern of the corresponding root of the parent plant with higher growth rate and productivity, are therefore extensively studied as an effective alternative for the in vitro production of these metabolites. Hairy roots are the transformed roots, generated from the infection site of the wounded plants with Agrobacterium rhizogenes. With their fast growth, being free from pathogen and herbicide contamination, genetic stability, and autotrophic nature for plant hormones, hairy roots are considered as useful bioproduction systems for specialized metabolites. Lately, several elicitation methods have been employed to enhance the accumulation of these compounds in the hairy root cultures for both small and large-scale production. Nevertheless, in the latter case, the cultivation of hairy roots in bioreactors should still be optimized. Hairy roots can also be utilized for metabolic engineering of the regulatory genes in the metabolic pathways leading to enhanced production of metabolites. The present study summarizes the updated and modern biotechnological aspects for enhanced production of secondary metabolites in the hairy root cultures of the plants of Solanaceae and their respective importance.

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Section: Culture Systems Used For Scaling Up Of Hairy Root Productionmentioning

confidence: 99%

Hairy root culture: a potent method for improved secondary metabolite production of Solanaceous plants

Biswas,

Chakraborty,

Mukherjee

et al. 2023

Front. Plant Sci.

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“…They reported that impaired oxygen transfer due to channelling and stagnation of the liquid phase are the apparent causes of poor growth [50]. Inclusion of polyurethane foam in the vessel of air-sparged bioreactor reduces the entrapping of gas by hairy roots, which improve biomass and alkaloid production [51]. In Artemisia annua hairy root culture, the bubble column reactor was superior to mist reactors for the biomass concentration [52,53].…”

Section: Bubble Column Reactormentioning

confidence: 99%

Types And Designs Of Bioreactors For Hairy Root Culture

Choi

Kim

Paek

Plan Tissue Culture Engineering

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Plant Cell Cultures, Secondary Product Accumulation

Pras

2000

Encyclopedia of Cell Technology

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Introduction Manipulation of Culture Conditions Cell Selection Induction of Differentiation in Plant Cell Cultures Bioconversion Genetic Approaches Conclusions Bibliography

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Characteristics of Scopolamine-releasing Hairy Root Clones ofDuboisia leichhardtii

Cited by 10 publications

References 8 publications

Hairy root culture: a potent method for improved secondary metabolite production of Solanaceous plants

Hairy root culture: a potent method for improved secondary metabolite production of Solanaceous plants

Types And Designs Of Bioreactors For Hairy Root Culture

Plant Cell Cultures, Secondary Product Accumulation

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