Characteristics of Staphylococcus aureus strains isolated from different animal species

Devriese, Luc; Oeding, Per

doi:10.1016/s0034-5288(18)33337-x

Cited by 63 publications

(54 citation statements)

References 19 publications

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“…The sizes of the PCR products ranged from approximately 1,200 to 1,600 bp. The (Devriese 1984) 100 strains were divided into seven different clusters (A-G) with seven different sizes of approximately 1,200, 1,250, 1,300, 1,450, 1,500, 1,550 and 1,600 bp. Gene polymorphisms of the X region of protein A are shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…We chose the biotyping technique established by Devriese (1984) as a conventional typing method. This simplified biotyping system has been useful in tracing the origin of S. aureus strains in animal feed and in the food industry (Kitai et al 2005).…”

Section: Discussionmentioning

confidence: 99%

“…Biotyping was performed according to the simplified system described by Devriese (1984), using four tests including staphylokinase production with bovine fibrinogen (Sigma), β-haemolysis on sheep blood agar (Merck), coagulation of bovine plasma for a duration of 6 h, and crystal violet growth types on Tryptose agar (Merck). All strains were classified to host specific ecovars and non-host specific (NHS) biotypes (Table 2).…”

Section: Biotypingmentioning

confidence: 99%

“…During the last few years, phenotypic methods have been replaced with molecular approaches for typing of S. aureus. However, biotyping using the simplified system published by Devriese (1984), indicates sources of S. aureus strains in animal foods. These methods could complement genotyping methods .…”

Section: Introductionmentioning

confidence: 99%

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Occurrence and molecular characterization of Staphylococcus aureus strains isolated from meat and dairy products by PCR-RFLP

et al. 2010

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A total of 100 Staphylococcus aureus strains were isolated from 1,047 food samples. In addition to biotyping all isolates, the occurrence of the tst gene and protein were evaluated by PCR and the RPLA test, respectively. Moreover, polymorphism of the X-region of the protein A gene was analyzed by PCR-RFLP. TSST 1 was detected in 12 strains and production of TSST 1 in all strains was confirmed by RPLA assay. It was noteworthy that 66.7% of TSST 1 -producing S. aureus strains belonged to the human biotype. The result of genotyping amplification of the spa gene displayed size polymorphisms and revealed seven different clusters ranging from 1,200 bp to 1,600 bp. Digestion of amplicons with HindIII and HaeII resulted in six distinct patterns for each of them. An amplicon of 1,450 bp in size was the predominant type of Spa-PCR product. The accuracy of the Spa typing system by PCR-RFLP was 100%, thus confirming this method as a reliable tool in routine epidemiological investigation for S. aureus.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Biotypingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Occurrence and molecular characterization of Staphylococcus aureus strains isolated from meat and dairy products by PCR-RFLP

et al. 2010

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“…At that time characterization of S. aureus isolates by means of phenotypical traits (phage typing, hemolysine types, coagulase types, staphylokinase etc.) suggested the existence of host-adapted so-called ecovars such as for humans, cattle, sheep, and chicken (Meyer, 1967;Há jek and Marsá lek, 1969;Witte et al, 1977;Witte et al, 1978;Devriese, 1984;Theakston et al, 1990). These techniques have been useful for classifying bacterial isolates into convenient intraspecific subsets but provided little information about genetic relationships of these ecovars.…”

Section: Staphylococcus Aureus and Host Specificitymentioning

confidence: 99%