Characteristics of the constituent substrains of Bacillus popilliae growing in batch and continuous cultures

Abstract

Continuous culture of Bacillus popilliae was achieved for the first time in a small chemostat. Initially, variable cell yields during steady-state chemostat growth led to a re-examination of growth rates in batch cultures. B. popilliae NRRL B-2309 and a wild strain were both found to be natural mixtures of three substrains characterized by different growth rates and colony morphologies and varying stability. Selected subcultures grown continuously provided data for three different cell production curves. Cell yields were two to three times greater per unit of medium in continuous than in batch culture, and about 1% of slow-growing chemostat cells formed typical spores.

The Use and Benefits of Bacillus Based Biological Agents in Aquaculture

“…[27,[128][129][130]146]. It has furthermore been widely documented that protein sources influence spore production in this species [141,[147][148][149]. Commonly used nitrogen based nutrient sources include a wide range of peptones, extracts and hydrolysates, many of which are too expensive for industrial scale manufacture of large volume products and have negative market acceptance if they contain animal by-products [143,150].…”