Characteristics of the interspecific somatic hybrids Solanum pinnatisectum (+) S. tuberosum H-8105

Szczerbakowa, Anna; Bołtowicz, Danuta; Lebecka, Renata; Radomski, Paweł; Wielgat, Bernard

doi:10.1007/s11738-005-0002-x

Cited by 15 publications

(10 citation statements)

References 20 publications

(17 reference statements)

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“…Low regeneration ability was also observed by Szczerbakowa et al (2005), whereby only two S. pinnatisectum + dihaploid H-8105 somatic hybrids were recovered. It was supposed that the extremely low frequency of the regenerated somatic hybrids could be a result of both low yield and the lack of morphogenetic capacity of leaf protoplasts in S. pinnatisectum.…”

Section: Discussionmentioning

confidence: 95%

Electrofusion of protoplasts from Solanum tuberosum, S. bulbocastanum and S. pinnatisectum

2008

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This paper discusses a number of experiments performed, involving the fusion by an electric field of mesophyll protoplasts from Solanum tuberosum cv. Bintje, S. tuberosum dihaploid clones 243, 299 and the wild tuberous disease-resistant species S. bulbocastanum and S. pinnatisectum. Three fusion experiments (S. bulbocastanum + S. tuberosum dihaploid 243, S. pinnatisectum + S. tuberosum cv. Bintje and S. pinnatisectum + S. tuberosum dihaploid 299) yielded 542 calli, the 52 ones of which produced shoots. Obtained regenerants were estimated by the flow-cytometry (FC) and RAPD analysis to determine hybrid plants.The utilisation of the FC as a useful method for detecting somatic hybrids is also discussed in this paper. The combination S. bulbocastanum + S. tuberosum dihaploid 243 led to the creation of eight somatic hybrids, the combination S. pinnatisectum + S. tuberosum cv. Bintje yielded four somatic hybrids and the combination S. pinnatisectum + S. tuberosum dihaploid 299 resulted in no hybrid regenerants. Morphology in vitro, growth vigour and production of tuber-like structures were evaluated in hybrid plants. Plants were transferred in vivo for further estimation (acclimatization, habitus evaluation and tuberization ability).

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Section: Discussionmentioning

confidence: 95%

Electrofusion of protoplasts from Solanum tuberosum, S. bulbocastanum and S. pinnatisectum

2008

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“…From them 23 were able to develop roots and only 12 were confirmed somatic hybrids of S. tuberosum and S. pinnatisectum. Low regeneration ability was corresponded to the somatic hybrids of S. pinnatisectum and dihaploid H-1805 produced by Szczerbakowa et al (2005). It was believed that the low frequency of regeneration of somatic hybrids could be a result of both low yield and lack of calli differentiation capacity of the mesophyll protoplast in parents (Szczerbakowa et al 2005).…”

Section: Discussionmentioning

confidence: 99%

“…Low regeneration ability was corresponded to the somatic hybrids of S. pinnatisectum and dihaploid H-1805 produced by Szczerbakowa et al (2005). It was believed that the low frequency of regeneration of somatic hybrids could be a result of both low yield and lack of calli differentiation capacity of the mesophyll protoplast in parents (Szczerbakowa et al 2005). Somatic hybrids were identified at molecular and morphological levels and ploidy level by flow cytometry estimation.…”

Section: Discussionmentioning

confidence: 99%

Production and characterization of somatic hybrids between Solanum tuberosum L. and S. pinnatisectum Dun.

Sarkar

Tiwari

Sharma

et al. 2011

Plant Cell Tiss Organ Cult

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Interspecific somatic hybrids between the dihaploid Solanum tuberosum and the wild species S. pinnatisectum Dun. were produced via protoplast fusion. Protoplast isolation, electrofusion, culture of post-fusion products and regeneration of calli/shoots were undertaken following optimized protocols. Regenerants were characterized for hybridity, ploidy and resistance to Phytophthora infestans (Mont.) de Bery, causal fungal pathogen of late blight disease. From a total of 126 regenerated macrocalli, 12 somatic hybrids were confirmed by possessing speciesspecific diagnostic bands of their corresponding parents as revealed by RAPD, SSRs and cytoplasmic-DNA analyses. Tetraploid status of the 12 hybrids was determined using flow cytometry analysis. Intermediate phenotypes for leaf, flower, and tuber characteristics and high male fertility were observed in field-grown hybrid plants. Hybrids were highly resistant to foliage late blight based on field assessment for two seasons. In contrast, moderate level of resistance to foliage blight was observed in hybrids based on the detached leaf assay under laboratory conditions.Overall, somatic hybrids with moderate levels of resistance to foliage blight were identified, and these will be useful for in situ hybridization in potato breeding efforts.

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“…S. pinnatisectum 8166 were evaluated in terms of organelle type, ploidy level, and phenotype of in vitro plants. On the whole, the morphology of somatic hybrids is intermediate between that of the two parents (Thieme et al 1997;Szczerbakowa et al 2005;Greplová et al 2008). The variability among somatic hybrids of the same origin can be seen in many traits, e.g., morphology, fertility, and degree of resistance to diseases (Thieme et al 1997;Carputo et al 2000a;Carrasco et al 2000;Oberwalder et al 1997;Oberwalder et al 2000;Szczerbakowa et al 2003aSzczerbakowa et al , 2005Borgato et al 2007;Thieme et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Early characterization of somatic hybrids from symmetric protoplast electrofusion of Solanum pinnatisectum Dun. and Solanum tuberosum L.

Polzerová

Patzak

Greplová

2010

Plant Cell Tiss Organ Cult

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Variability of 31 somatic hybrids of Solanum pinnatisectum Dun. with Solanum tuberosum L. for leaf morphology, plant vigor, resistance to Phytophthora infestans, ploidy level, and cytoplasm type was evaluated in vitro. The composition of these somatic hybrids was as follows: [S. pinnatisectum Dun. (2n = 2x = 24; cytoplasmic type Wc) ? S. tuberosum L. (2n = 4x = 48; cytoplasmic type Tß)]. Based on leaf morphology and plant growth vigor, plants were divided into three groups, including plants close to tbr parent with unlobed leaves, small plants with scarcely dissected leaves, and vigorous plants with asymmetrically and pinnately lobed leaves. Nine of the somatic hybrids were found to be highly resistant to P. infestans. Somatic hybrids were either tetraploid or hexaploid, with hexaploids being predominant. The cytoplasm of somatic hybrids was either Tßc or Wßc, with Tßc being more common. Overall, in contrast to leaf morphology and growth vigor, level of resistance to P. infestans was not related to either ploidy level or type of cytoplasm. These findings demonstrate that early in vitro selection of promising hybrids can be useful in breeding programs.

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Characteristics of the interspecific somatic hybrids Solanum pinnatisectum (+) S. tuberosum H-8105

Cited by 15 publications

References 20 publications

Electrofusion of protoplasts from Solanum tuberosum, S. bulbocastanum and S. pinnatisectum

Electrofusion of protoplasts from Solanum tuberosum, S. bulbocastanum and S. pinnatisectum

Production and characterization of somatic hybrids between Solanum tuberosum L. and S. pinnatisectum Dun.

Early characterization of somatic hybrids from symmetric protoplast electrofusion of Solanum pinnatisectum Dun. and Solanum tuberosum L.

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