Characteristics of Tumors Induced in Mammals, Especially Rodents, by Viruses of the Avian Leukosis Sarcoma Group

Simkovĭc, D

doi:10.1016/s0065-3527(08)60748-4

Cited by 21 publications

(15 citation statements)

References 61 publications

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“…(3) Non-inducible NP cells, also called non-virogenic (cf. reviews by Svoboda, 1968, andSimkovic, 1972. For non-virogenic cells, cf.…”

Section: Discussionmentioning

confidence: 99%

“…Ve proteins have also been found in some of these lines (cf. Svoboda, 1968;Simkovic, 1972). Since the antisera used were not monospecific, the tests could not indicate precisely which of the p and gp proteins were present in the cells.…”

Section: Discussionmentioning

confidence: 99%

“…However, this correlation was not found in other lines of ASV-transformed mammalian cells (cf. Simkovic, 1972).…”

Section: Discussionmentioning

confidence: 99%

“…The TWERC cells have been shown to contain viral gs antigen(s) and to carry TSSA at their surface . However, no virus could be induced by grafting TWERC cells to chicken or associating them with CEF (Simkovic 1972). The LWF line of rat embryo fibroblasts (Lewis inbred strain, Hlubinova and Simkovic, 1972;Aupoix et al, 1973) served as control for the transformed rat cells.…”

Section: Cellsmentioning

confidence: 99%

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Expression of viral proteins in mammalian cells transformed by avian sarcoma viruses

Aupoix¹,

Vigier²

1976

Intl Journal of Cancer

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The expression of viral proteins in nine lines of hamster and rat cells transformed by avian sarcoma viruses (ASV) was studied by indirect immunofluorescence with monospecific antisera to purified gp85 and p27 of AMV-B and a polyvalent antiserum to all the p proteins of this same virus. The lines of ASV-transformed cells were either low virus producers (VP) or inducible or non-inducible non producers (NP). Cytoplasmic expression of p proteins was observed in all the cell lines except the least inducible NP cell line, and cytoplasmic expression of gp85 in all the cell lines. The degree of expression varied widely with the lines and was not related to the class of permissiveness or inducibility. However, in the inducible NP class, the expression of p proteins and gp85 was higher in the most inducible cell lines. The data also suggest that the expression of the p proteins must be uncoordinate in at least some cell lines and must also be uncoordinate with the expression of gp85. In the VP cell lines and the most inducible NP lines, g85 and some p proteins other than p27 were also expressed on the cell membrane. The membrane expression of gp85 and the p proteins which were expressed appeared to be coordinate and to parallel the degree of cytoplasmic expression. In contrast, no, or a negligible expression of viral proteins was observed on the membrane of the least inducible and the non-inducible cell lines. These results suggest that there may exist translational and/or post-translational controls of the expression of viral proteins in the ASV-transformed mammalian cells and that the permissiveness and the inducibility of the cells may depend on the insertion of viral proteins in the cell membrane. The failure of p27 to insert in the cell membrane could account for the low permissiveness or the non-permissiveness of the cells.

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“…(3) Non-inducible NP cells, also called non-virogenic (cf. reviews by Svoboda, 1968, andSimkovic, 1972. For non-virogenic cells, cf.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…However, this correlation was not found in other lines of ASV-transformed mammalian cells (cf. Simkovic, 1972).…”

Section: Discussionmentioning

confidence: 99%

Section: Cellsmentioning

confidence: 99%

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Expression of viral proteins in mammalian cells transformed by avian sarcoma viruses

Aupoix¹,

Vigier²

1976

Intl Journal of Cancer

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“…Host-induced alterations in viral biological properties have also been noted in other oncornaviruses replicated in heterologous hosts (6,13,31,34 …”

mentioning

confidence: 95%

Host induced alteration of avian sarcoma virus B-77 genome.

Shoyab¹,

Markham²,

Baluda³

1975

Proc. Natl. Acad. Sci. U.S.A.

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The genome of an avian oncornavirus was altered after infection of a heterologous host. This was studied with avian sarcoma virus B-77 in duck embryonic fibroblasts (DEF) and chicken embryonic fibroblasts (CEF). To detect alteration of the viral genome, we hybridized 35S B-77 RNA with normal duck DNA by either one of two techniques: when viral RNA was in excess and when DNA was in excess. The RNA of B-77 passaged only in gs-chf-CEF does not have homology with duck DNA. However, after several passages of B-77 through DEF the viral genome acquired duck specific RNA sequences. After 4 and 10 passages, B-77 RNA acquired 2.2 and 6.6%, respectively, complementarity to normal duck DNA. The duck specific RNA sequences were found to be covalently linked to the B-77 RNA genome. Also, the host specific sequences acquired by the virus appear to be from a region of the duck DNA which is repeated four to six times per cell. After 5 back passages in CEF some of the duck specific RNA sequences in the viral genome were lost.Avian oncornaviruses have the ability to be modified by the host through which they are passaged. Even Rous sarcoma virus (RSV) may have had to be adapted to induce tumors and replicate in chickens (1). After the isolation of chicken tumor no. 1 by Rous in 1911, the tumor cells could cause sarcomas only in chickens of the same strain as that in which the tumor originated and only by inoculation of large numbers of tumor cells. Subsequently, tumors could be induced in other strains of chickens but only after several more passages could sarcomas be induced by a cell-free filtrate. After more than 60 years of laboratory manipulations, RSV can now rapidly induce tumors in a wide host range including primates (2, 3). DuranReynals reported that after several passages in ducks, a duck adapted variant of RSV appeared (4). In addition to the ability to cause tumors in ducks, the duck-adapted virus had acquired new tissue affinities causing tumors in bone, skin, and digestive tract, whereas the original RSV did not. Furthermore, the duck-adapted virus had altered surface antigenic properties. A similar phenomenon has also been noted with RSV that has been rescued from transformed rat cells (5-12). These findings led Altaner and Temin (6) to suggest that the alteration of physical and biological properties in the virus may reflect recombination between viral and host genetic material. Host-induced alterations of murine sarcoma viruses have also been reported after passage in rat cells (13). Kirsten and Harvey sarcoma viruses appear to contain murine and rat oncornavirus specific RNA sequences but these sequences have Abbreviations: DEF, duck embryo fibroblasts; CEF, chicken embryo fibroblasts; RSV, Rous sarcoma virus; AMV, avian myeloblastosis virus; Cot, nucleotide concentration (mol/l) X time (sec); TCA, trichloroacetic acid.1031 not been shown to be covalently linked. Also, in those studies the DNA probe synthesized in vitro with reverse transcriptase may have contained cellular as well as viral sequences sin...

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Biology of Avian Retroviruses

Payne

1992

The Retroviridae

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Characteristics of Tumors Induced in Mammals, Especially Rodents, by Viruses of the Avian Leukosis Sarcoma Group

Cited by 21 publications

References 61 publications

Expression of viral proteins in mammalian cells transformed by avian sarcoma viruses

Expression of viral proteins in mammalian cells transformed by avian sarcoma viruses

Host induced alteration of avian sarcoma virus B-77 genome.

Biology of Avian Retroviruses

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