No abstract

SUMMARYTwo established rat cell lines, WERC and TWERC, were studied for the presence of C-type RNA virus particles. WERC cells, spontaneously transformed in vitro, continuously released C-type RNA virus (WERC virus) by budding from the plasma membrane. Some of these virus particles (diameter about Ioo nm.) showed an elecron-lucent nucleoid of diameter 75 to 8o nm. and represented immature C-type virus. Others showed dense nucleoids of about 65 to 7 ° nm. diameter and looked like mature forms of routine leukaemia virus. Each cell showed 6o to IOO virus particles. The buoyant density of the virus particles in sucrose gradients was that expected for avian or murine RNA virus particles (I" i6 g./ml.).No virus particles were observed in the subline of cells (TWERC) originating from the malignant transformation with avian sarcoma virus (the PRAGUE strain of Rous sarcoma virus) of parental WERC cells.

Both immunoscintigraphy and RIGS enable one to make a more accurate diagnosis. While treating the primary disease the use of RIGS may help in assessment of necessary extent of operation performance and in staging of the disease by revealing occult lymph nodes involved. Pre-operative immunoscintigraphy seems to be a useful diagnostic method for detection of tumour recurrence. When comparing two radiopharmaceuticals used, CEA-Scan seems to be more suitable for diagnostic studies, but using the Oncoscint for tumour recurrence detection had some specific benefit, too.

Intraoperative enteroscopy is accepted as the ultimate diagnostic procedure for complete investigation of the small bowel. Despite the introduction of double-balloon enteroscopy into clinical practice, intraoperative enteroscopy will be reserved for those cases where double-balloon enteroscopy cannot be performed or fails to investigate the entire small intestine, especially to prevent excessive bowel resection.