2017
DOI: 10.1371/journal.pone.0170479
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Characteristics Peripheral Blood IgG and IgM Heavy Chain Complementarity Determining Region 3 Repertoire before and after Immunization with Recombinant HBV Vaccine

Abstract: Immunization with recombinant HBV vaccine induces specific immune responses in human causing B lymphocytes to produce protective HBsAb, and to form memory B lymphocytes, thereby facilitating HBV immunity in the body. However, B lymphocytes heterogeneity and characteristics are not fully elucidated. In this study, we conducted high-throughput sequencing of BCR heavy chain CDR3 repertoires in 3 healthy volunteers before and after the third immunization with recombinant HBV vaccine. We used Roche 454 Genome Seque… Show more

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Cited by 15 publications
(13 citation statements)
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“…Our observation that every donor's repertoire is rather unique is in line with previous findings from single cell B cell studies (56, 7984) as well as from other HTS studies (50, 68, 72, 8590).…”
Section: Discussionsupporting
confidence: 91%
“…Our observation that every donor's repertoire is rather unique is in line with previous findings from single cell B cell studies (56, 7984) as well as from other HTS studies (50, 68, 72, 8590).…”
Section: Discussionsupporting
confidence: 91%
“…The subjects included six healthy volunteers (6 samples: H-1, H-2, H-3, H-4, H-5 and H-6) (22), two volunteers with systemic lupus erythematosus (SLE) (including 6 total samples pretreatment, during treatment and after treatment, namely, S1-1, S1-2, S1-3, S2-1, S2-2 and S2-3) (23), three volunteers with breast cancer (including 9 total samples pretreatment, during treatment and after treatment, namely, B3-1, B2-1, B1-1, B3-2, B2-2, B1-2, B3-3, B2-3, and B1-3), two volunteers with a high titer of HBsAb (2 samples: HBsAb-1, HBsAb-2) (24) and three volunteers with samples before and after immunization with the HBV vaccine (6 IgM samples (V1-BM, V1-AM, V2-BM, V2-AM, V3-BM, V3-AM) and 6 IgG samples (V1-BG, V1-AG, V2-BG, V2-AG, V3-BG, and V3-AG)) (25). The peripheral blood samples were obtained from the Affiliated Hospital of Zunyi Medical University.…”
Section: Subjects and Sample Preparationmentioning
confidence: 99%
“…Error from bias in this multiplex PCR assay was controlled using synthetic templates (26), and the HCDR3 sequences were acquired by HTS on the ImmunoSEQ platform (http://www.adaptivebiotech.com) (23). All the PCR products of cDNA samples after PCR amplification were sent to Tongji-SCBIT Biotechnology Corporation for HTS, and detailed experimental procedures have been described in our previous article (25). The HCDR3 regions were identified within the sequencing reads according to the definition established by the International ImMunoGeneTics (IMGT) collaboration.…”
Section: High-throughput Sequencingmentioning
confidence: 99%
“…Characterization of Ag-specific BCR repertoires is essential for understanding disease mechanisms involving humoral immunity. A study by Ma et al (22) suggests that the mechanism of high-frequency CDR3 generation is associated with the maturation of IgG affinity (somatic hypermutation) during the recombinant HBV vaccine-induced B-lymphocyte responses. In the present study, using high-throughput sequencing, the BCR H-CDR3 region was analyzed in the DNA from PBMCs of 3 kidney transplant patients with typical acute rejection after kidney transplantation.…”
Section: Discussionmentioning
confidence: 99%