Limited water resources and ensuring access to clean water are critical environmental challenges, especially for the developing world. In particular, rural domestic wastewater has become a significant source for the pollution of freshwater bodies. A modified bio-ecological A2O-wetland system for rural wastewater treatment consisting of a biological unit (anaerobic baffled reactor, anoxic tank and oxic unit, A2O) and an ecological unit (horizontal flow constructed wetland) was developed, and key performance indicators were identified. The bio-ecological treatment system showed high removal efficiency for pollutants, successfully achieving 91%, 85%, 78%, and 92% removal efficiencies for chemical oxygen demand (COD), ammonium (NH 4 -N), total nitrogen (TN), and total phosphorus (TP), respectively. The concentrations of pollutants in the effluent from the system were lower than the Class 1 A regulated values of the Chinese National Standard GB18918-2002. The system offered high removal efficiency, simple operation, and low energy consumption. The A2O-wetland is a good alternative for rural wastewater treatment systems.