Characterization and Analysis of the Temporal and Spatial Dynamic of Several Enteritis Modeling Methodologies

Abstract

Inflammatory bowel disease (IBD), such as Crohn’s disease and ulcerative colitis, is a complex disease involving genetic, immune, and microbiological factors. A variety of animal models of IBD have been developed to study the pathogenesis of human IBD, but there is no model that can fully represent the complexity of IBD. In this study, we established two acute enteritis models by oral 3% DSS or intraperitoneal injection of anti-CD3 antibody, and two chronic enteritis models by feeding 3 cycles of 1.5% DSS or 3 months of the high-fat diet, respectively, and then examined the clinical parameters, histological changes, and cytokine expression profiles after the successful establishment of the models. Our results indicated that in 3% DSS-induced acute enteritis, the colorectal injury was significantly higher than that of the small intestine, while in anti-CD3 antibody-induced acute enteritis, the small intestine injury was significantly higher than that of colorectal damage. Besides, in the 1.5% DSS-induced chronic enteritis, the damage was mainly concentrated in the colorectal, while the damage caused by long-term HFD-induced chronic enteritis was more focused on the small intestine. Therefore, our work provides a reference for selecting appropriate models when conducting research on factors related to the pathogenesis of IBD or evaluating the potential diagnosis and treatment possibilities of pharmaceuticals.

Regulation of Leucine-Rich Repeat Kinase 2 by inflammation and IL-4

“…To understand how inflammation affects LRRK2 in immune cells, we isolated splenocytes 24h after intraperitoneal injection of anti-CD3 antibodies that activates T-cells to release cytokines, driving transient inflammation (Swamy et al , 2015; Xu et al , 2021; Yaguchi et al , 2004; Zhou et al , 2004). This treatment induced inflammation apparent by significant change in cellular composition in the spleen ( Suppl.…”

The mitigative effect of ovotransferrin-derived peptide IQW on DSS-induced colitis via alleviating intestinal injury and reprogramming intestinal microbes

“…IBD is a multifactorial disease with multiple complications, which make the diagnosis and treatment of IBD a major challenge. The DSS-induced mouse colitis model has similar clinical symptoms of colitis, such as weight loss, metabolic disorders, and intestinal flora disorders (20)(21)(22). In this study, the effects and possible mechanisms for the relief of colitis were investigated by administering different concentration gradients of IQW in the context of DSS induction.…”

Different depths of food restriction and high‐fat diet refeeding in mice impact host obesity and metabolic phenotypes with correlative changes in the gut microbiota

“…Samples were quantified for the relative quantity of gene expression. 35 The primers used in the quantitative real‐time PCR are listed in Table S1 .…”